胚性線維芽細胞培養上清によるニワトリ始原生殖細胞の生存性と増殖性の向上

概要

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Primordial germ cells circulating in the embryonic vascular system (cPGCs) were isolated from 2.5-day-old chick embryos and cultured individually in suspension for 5 days in embryonic stem-cell medium (ESM) adjusted to pH 8.0 supplemented with fetal bovine serum. Most cells died within 5 days, during which time only a few cells (16-23%) underwent mitosis once, and only a very few cells (less than 3%) twice. The use of conditioned medium (CM) derived from embryonic fibroblasts enhanced the viability and proliferation of the cPGCs, resulting in survival of up to one-third of the cells for 5 days. A fraction of the cells (3-5%) even divided 3 times. The in vitro cultured cPGCs maintain the capacity to migrate into the gonadal area. The chick cPGCs cultured in vitro for up to 5 days in the CM produced germ-line chimeras upon injection into the bloodstream of 2-day-old quail embryos.
日本繁殖生物学会の論文