電気的活性化後のブタ体外成熟卵の発生能力
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概要
- 論文の詳細を見る
Developmental competence of in vitro matured porcine oocytes obtained by TCM199- and NCSU 23-based IVM systems after electrical activation were compared. When the nuclear phase of granulosa-cumulus-oocyte complexes (GCOCs) used in the TCM199-based IVM system and cumulus-oocyte complexes (COCs)used in the NCSU 23-based IVM system were observed, most of GCOCs were fully grown dictyate arrest oocytes (GV I, 68.6%), on the other hand, only 33.8% of COCs were at GV I stage. There were no differences in the rates of maturation (93.2%, 82/88 vs 91.3%, 63/69), normal cleavage (70.7%, 58/82 vs 65.1%, 41/63) and development to blastocysts (37.8%, 31/82 vs 34.9%, 22/63) between the in vitro matured oocytes obtained by the two IVM systems. However, the parthenogenetic blastocysts derived from the NCSU23-based IVM system had higher cell numbers than the blastocysts derived from the TCM199-based IVM system (35.6 ± 4.5 vs 22.6 ± 2.0; P<0.01). Transfer of parthenogenetic oocytes derived from the NCSU23-based IVM system resulted in a high developmental rate to somite stage fetuses (26.9%, 45/167). These data demonstrate that the activated porcine oocytes derived from the two IVM systems had equal ability of in vitro development, though there was a difference in the cell number of the parthenogenetic blastocysts. It was also shown that in vitro matured oocytes obtained by the NCSU23-based IVM system had excellent ability of parthenogenetic development to fetuses, indicating that they are usable in porcine developmental engineering.
- 日本繁殖生物学会の論文
著者
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Kano Koichiro
Department of Animal Sciences, College of Bioresource Sciences, Nihon University
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NAGASHIMA Hiroshi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
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黒目 麻由子
明治大学農学部生命科学科発生工学研究室
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KUROME Mayuko
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
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WAKO Naohiro
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
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OCHIAI Takashi
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
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MIYAGAWA Shuji
Division of Organ Transplantation, Biomedical Research Center, Osaka University Graduate School of M
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Miyagawa Shuji
Division Of Organ Transplantation Department Of Molecular Therapeutics Osaka University Graduate Sch
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Kano Koichiro
Department Of Animal Science Faculty Of Agriculture Tohoku University
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栗原 隆
大阪大学大学院・医学系研究科臓器制御医学専攻
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栗原 隆
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate Sc
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黒目 麻由子
Laboratory of Reproduction Engineering, Department of Life Science, Meiji University
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若生 直浩
Laboratory of Reproduction Engineering, Department of Life Science, Meiji University
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MIZUNO Kenichi
Laboratory of Reproduction Engineering, Department of Life Science, Meiji University
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FUJIMURA Tatsuya
The Animal Engineering Research Institute Co., Ltd
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TAKAHAGI Yoichi
The Animal Engineering Research Institute Co., Ltd
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MURAKAMI Hiroshi
The Animal Engineering Research Institute Co., Ltd
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SHIRAKURA Ryota
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate Sc
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Fujimura Tatsuya
The Animal Engineering Research Institute
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Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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若生 直浩
明治大学農学部・生命科学科生殖工学研究室
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Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Ochiai Takashi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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KURIHARA Takashi
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate Sc
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Nagashima Hiroshi
Laboratory For Evolutionary Morphology Riken Cdb
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TAKAHAGI Yoichi
The Animal Engineering Research Institute
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SHIRAKURA Ryota
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine
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MIYAGAWA Shuji
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine
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KANO Koichiro
Department of Animal Science, College of Bioresource Sciences, Nihon University
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KURIHARA Takashi
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine
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