ヒト血清アルブミンとEGFPを発現する形質転換ブタの開発
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概要
- 論文の詳細を見る
We introduced a fusion gene of human albumin and enhanced green fluorescent protein (EGFP) into porcine oocytes using the sperm vector method, and produced a piglet that showed clear expression of GFP in the hooves and skin. PCR and Southern blotting analysis of genomic DNA extracted from the piglets tissues, including the liver, showed that the tissues carried the transgene. RT-PCR analysis demonstrated that both the human albumin and EGFP genes were expressed in the tissues. The fact that human albumin gene was integrated and expressed in the liver of the transgenic pig opened a way for us to achieve our goal, which was the use of transgenic pigs for the bioartificial liver support system.
- 日本繁殖生物学会の論文
著者
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成瀬 勝俊
東京大学医学部附属病院肝胆膵・人工臓器移植外科
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成瀬 勝俊
東京大学肝胆膵人工臓器移植外科
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NAGASHIMA Hiroshi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
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KUROME Mayuko
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
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成瀬 勝俊
Division of Artificial Organs & Transplantation, Department of Surgery, Faculty of Medicine, Univers
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石川 博
SRL Inc.
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河野 博臣
Laboratory of Developmental Engineering, Department of Developmental Engineering, School of Agricult
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UEDA Hideto
Laboratory of Developmental Engineering, Department of Developmental Engineering, School of Agricult
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MIYAZAKI Koji
R Laboratory, Nipro Corporation
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ENDO Maiko
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
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SAWASAKI Tohru
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
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MAKUUCHI Masatoshi
Division of Artificial Organs & Transplantation, Department of Surgery, Faculty of Medicine, Univers
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NARUSE Katsutoshi
Division of Artificial Organs and Transplantation, Department of Surgery, Faculty of Medicine, Unive
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Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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ISHIKAWA Hiroshi
SRL Inc.
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KAWANO Hiro-omi
Laboratory of Developmental Engineering, Department of Developmental Engineering, School of Agricult
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MIYAZAKI Koji
R&D Laboratory, Nipro Corporation
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Ueda Hideto
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Endo Maiko
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Sawasaki Tohru
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Naruse Katsutoshi
Division Of Artificial Organs And Transplantation Department Of Surgery Faculty Of Medicine Universi
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Kawano Hiro-omi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Nagashima Hiroshi
Laboratory For Evolutionary Morphology Riken Cdb
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UEDA Hideto
Laboratory of Developmental Engineering, Department of Developmental Engineering, School of Agriculture, Meiji University
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KUROME Mayuko
Laboratory of Developmental Engineering, Department of Developmental Engineering, School of Agriculture, Meiji University
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MIYAZAKI Koji
R&D Laboratory, Nipro Corporatio
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NAGASHIMA Hiroshi
Laboratory of Developmental Engineering, Department of Developmental Engineering, School of Agriculture, Meiji University
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