Production of Transgenic and Non-Transgenic Clones in Miniature Pigs by Somatic Cell Nuclear Transfer
スポンサーリンク
概要
- 論文の詳細を見る
Miniature pigs have been recognized as valuable experimental animals in various fields such as medical and pharmaceutical research. However, the amount of information on somatic cell cloning in miniature pigs, as well as genetically modified miniature pigs, is much less than that available for common domestic pigs. The objective of the present study was to establish an efficient technique of cloning miniature pigs by somatic cell nuclear transfer. A high pregnancy rate was achieved following transfer of parthenogenetic (3/3) and cloned (5/6) embryos using female miniature pigs in the early pregnancy period as recipients after estrus synchronization with prostaglandin F2 alpha analog and gonadotrophins. The production efficiency of the cloned miniature pigs using male and female fetal fibroblasts as nucleus donors was 0.9% (2/215 and 3/331, respectively). Cloned miniature pigs were also produced efficiently (7.8%, 5/64) by transferring reconstructed embryos into the uteri of common domestic pigs. When donor cells transfected with the green fluorescent protein (GFP) gene were used in nuclear transfer, the production efficiency of the reconstructed embryos and rate of blastocyst development were comparable to those obtained by non-transfected cells. When transfected cell-derived reconstructed embryos were transferred to three common domestic pig recipients, all became pregnant, and a total of ten transgenic cloned miniature pigs were obtained (piglet production efficiency: 2.7%, 10/365). Hence, we were able to establish a practical system for producing cloned and transgenic-cloned miniature pigs with a syngeneic background.
- 日本繁殖生物学会の論文
- 2008-06-01
著者
-
YAZAWA Hajime
Laboratory Animal Research Station, Nippon Institute for Biological Science
-
UENO Satoshi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
-
NAGASHIMA Hiroshi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
-
TOMII Ryo
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
-
KUROME Mayuko
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
-
Ueno Satoshi
Lab. Of Developmental Engineering Dep. Of Life Sciences School Of Agriculture Meiji Univ.
-
Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
-
Tomii Ryo
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
-
Ueno Satoshi
Laboratory Of Developmental Engineering Department Of Life Science Meiji University
-
Shimada Arata
Laboratory Of Developmental Engineering Department Of Life Science Meiji University
-
Shimada Arata
Laboratory Of Cell And Developmental Biology Faculty Of Bioscience And Biotechnology Tokyo Institute
-
Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
-
Yazawa Hajime
Laboratory Animal Research Station Nippon Institute For Biological Science
-
ISHIKAWA Takayuki
Laboratory Animal Research Station, Nippon Institute for Biological Science
-
Nagashima Hiroshi
Lab. Of Developmental Engineering Dep. Of Life Sciences School Of Agriculture Meiji Univ.
-
Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
-
Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
-
Nagashima Hiroshi
Laboratory For Evolutionary Morphology Riken Cdb
-
Ishikawa Takayuki
Laboratory Animal Research Station Nippon Institute For Biological Science
関連論文
- 卵細胞質内注入法を用いた牛の核移植
- Differential Development of Sex-related Characters of Chickens from the GSP and PNP/DO Inbred Lines after Left Ovariectomy
- Developmental Ability of Porcine In Vitro Matured Oocytes at the Meiosis II Stage After Vitrification
- Production of Cloned Pigs by Nuclear Transfer of Preadipocytes Following Cell Cycle Synchronization by Differentiation Induction
- Cloned Porcine Embryos can Maintain Developmental Ability after Cryopreservation at the Morula Stage
- Production Efficiency and Telomere Length of the Cloned Pigs Following Serial Somatic Cell Nuclear Transfer
- ヒト血清アルブミンとEGFPを発現する形質転換ブタの開発
- 電気的活性化後のブタ体外成熟卵の発生能力
- ニワトリ近交系GSP,GSN/1,PNP/どおよびBM-C系における,赤血球抗原変異検索,リンパ球混合培養法および皮膚移植法による主要組織適合遺伝子複合体抗原の均一性の評価
- Calcium Release at Fertilization : Artificially Mimicking the Oocyte's Response to Sperm
- ブタ卵の成熟条件がMII期紡錘体の形態に及ぼす影響
- Dependence of removal of sperm-specific proteins from Xenopus sperm nuclei on the phosphorylation state of nucleoplasmin
- Production of Transgenic and Non-Transgenic Clones in Miniature Pigs by Somatic Cell Nuclear Transfer
- Production of Cloned Pigs by Nuclear Transfer of Preadipocytes Following Cell Cycle Synchronization by Differentiation Induction
- Effects of Sperm Pretreatment on Efficiency of ICSI-Mediated Gene Transfer in Pigs
- Association Between Embryonic Loss and Damage to the Zona Pellucida by Invasive Micromanipulation During Oviductal Transfer of Early-Stage Embryos in Pigs
- Effects of Maturation Conditions on Spindle Morphology in Porcine MII Oocytes
- Effects of Recloning on the Efficiency of Production of α1,3-Galactosyltransferase Knockout Pigs
- Production of a Transgenic Pig Expressing Human Albumin and Enhanced Green Fluorescent Protein
- Autopodial Development in the Sea Turtles Chelonia mydas and Caretta caretta
- Application of Genetically Modified and Cloned Pigs in Translational Research
- In Vitro Development and Postvitrification Survival of Cloned Feline Embryos Derived from Preadipocytes
- Development and Perspectives of Perfusion Treatment for Liver Failure
- Cloned Porcine Embryos can Maintain Developmental Ability after Cryopreservation at the Morula Stage
- What Triggered Turtle Shell Evolution? : Identification of Gene Co-Option in Carapace Formation(Organogenesis and Evolution,Symposium,PROCEEDING OF THE 75^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
- The Production of a Diabetic Mouse Using Constructs Encoding Porcine Insulin Promoter-Driven Mutant Human Hepatocyte Nuclear Factor-1α
- Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device
- Hollow Fiber Vitrification : A Novel Method for Vitrifying Multiple Embryos in a Single Device
- Production of Diabetic Offspring Using Cryopreserved Epididymal Sperm by In Vitro Fertilization and Intrafallopian Insemination Techniques in Transgenic Pigs
- Matrix-Metalloproteinases-2 and -9 Production in Bovine Endometrial Cell Culture.
- In Vitro Development of Porcine Nuclear Transfer Embryos Reconstructed by Intracytoplasmic Microinjection of Cumulus Cell Nuclei into In Vitro Matured Oocytes.
- Evaluation of the Quality of Porcine Somatic Cell Nuclear Transfer Embryo by Gene Transcription Profiles
- The Production of a Diabetic Mouse Using Constructs Encoding Porcine Insulin Promoter-Driven Mutant Human Hepatocyte Nuclear Factor-1.ALPHA.
- Production of Transgenic-cloned Pigs Expressing Far-red Fluorescent Protein, Monomeric Plum