Cloned Porcine Embryos can Maintain Developmental Ability after Cryopreservation at the Morula Stage
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概要
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The aim of the present study was to clarify the overall efficiency of porcine somatic cell nuclear transfer (SCNT) by incorporating cryopreservation of the cloned embryos before transfer. The SCNT embryos reconstructed with preadipocytes and in vitro-matured (IVM) oocytes were cultured to harvest morula stage embryos; they were then subjected to delipation (removal of cytoplasmic lipid droplets) and vitrification. After warming and culture, the embryos developing to blastocysts were transferred to recipients to obtain cloned piglets. From 372 reconstructed embryos, 188 (50.5%) reached the morula stage and 117 (31.5%) developed to blastocysts after vitrification. Transfer of 98 (26.3%) morphologically normal blastocysts gave rise to 6 (1.6%) piglets, including 1 stillborn. The efficiency of the cloned piglet production was comparable with that obtained using SCNT embryos without cryopreservation (2.7%, 17/635). Here, we demonstrate that porcine somatic cell cloning can be performed without a significant reduction in efficiency even when the SCNT embryos are cryopreserved before transfer.
- 2011-04-01
著者
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MATSUNARI Hitomi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
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NAKANO Kazuaki
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
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TAKAHASHI Masashi
National Agricultural Research Center for Kyushu Okinawa Region
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Nakayama Naoki
Laboratory Of Developmental Engineering Department Of Life Sciences School Of Agriculture Meiji Univ
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MATSUMOTO Mitsuhito
National Agricultural Research Center for Kyushu Okinawa Region
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MURAKAMI Hitoshi
National Agricultural Research Center for Kyushu Okinawa Region
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KAJI Yuji
National Agricultural Research Center for Kyushu Okinawa Region
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Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Kaji Yuji
National Agricultural Res. Center For Kyushu Okinawa Region
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Ogawa Buko
Laboratory Of Developmental Engineering Department Of Life Sciences School Of Agriculture Meiji Univ
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Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Nagashima Hiroshi
Lab. Of Developmental Engineering Dep. Of Life Sciences School Of Agriculture Meiji Univ.
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Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Murakami Hitoshi
Laboratory Of Developmental Engineering Department Of Life Sciences School Of Agriculture Meiji Univ
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Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Matsunari Hitomi
Laboratory Of Developmental Engineering Department Of Life Sciences School Of Agriculture Meiji Univ
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Nagashima Hiroshi
Laboratory For Evolutionary Morphology Riken Cdb
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Ogawa Buko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
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Nakano Kazuaki
Laboratory Of Developmental Engineering Department Of Life Sciences School Of Agriculture Meiji Univ
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