Effects of Purple Sweet Potato Anthocyanins on Development and Intracellular Redox Status of Bovine Preimplantation Embryos Exposed to Heat Shock
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概要
- 論文の詳細を見る
The development of cleavage stage preimplantation embryos is disrupted by exposure to heat shock, such as high temperatures in the summer season. In this study, we investigated whether addition of anthocyanins, which are strong scavengers of reactive oxygen species (ROS), improves development and intracellular redox status of heat-exposed bovine preimplantation embryos by reduction of heat shock-derived oxidative stress. After in vitro fertilization, embryos were cultured at 38.5 C through Day 8 (Day 0=day of IVF) with 0, 0.1, 1, and 10 μg/ml anthocyanins (non-heat-shocked group). On Day 2, embryos were cultured at 41.5 C for 6 h with 0, 0.1, 1, and 10 μg/ml anthocyanins followed by culture at 38.5 C until Day 8 (HS group). After exposure to heat shock, the intracellular ROS and glutathione (GSH) contents of individual embryos were measured in the non-heat-shocked and HS groups using fluorescent probes. On Day 8, the blastocysts formation rates of the embryos and total cell numbers of blastocysts were evaluated. Embryos exposed to heat shock without anthocyanins showed a significant decrease in blastocyst formation rate and GSH content (p<0.05) and an increase in intracellular ROS (p<0.05) compared with non-heat-shocked embryos. In contrast, addition of 0.1 μg/ml anthocyanins significantly (p<0.05) improved the blastocyst formation rate of the heat-shocked embryos. Addition of any dose of anthocyanins produced a significant decrease in the ROS levels (p<0.05) and tended to increase the GSH levels under heat-shock conditions. However, addition of higher concentrations (1 and 10 μg/ml) of anthocyanins to the culture media under heat shock did not improve the development of embryos. These results indicate that anthocyanins maintain the intracellular redox balance of heat-shocked bovine embryos by reducing intracellular oxidative stress and increasing the GSH levels. Thus, alterations of the redox state using natural antioxidative polyphenols is a useful approach for reducing heat shock-derived oxidative stress.
- 日本繁殖生物学会の論文
著者
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KOBAYASHI Shuji
National Livestock Breeding Center
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SAKATANI Miki
National Agricultural Research Center for Kyushu Okinawa Region
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TAKAHASHI Masashi
National Agricultural Research Center for Kyushu Okinawa Region
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Oki Tomoyuki
National Agricultural Research Center For Kyushu Okinawa Region National Agricultural Research Organ
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Oki Tomoyuki
National Agricultural Research Center For Kyushu Okinawa Region
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Sakatani Miki
National Agricultural Res. Center For Kyushu Okinawa Region
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SUDA Ikuo
National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Bio-Orient
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Suda Ikuo
National Agricultural Research Center For Kyushu Okinawa Region National Agricultural Research Organ
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Kobayashi Shu-ichi
National Livestock Breeding Center
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Kobayashi Shu-ichi
National Agricultural Research Center For Kyushu Okinawa Region
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SUDA Ikuo
National Agricultural Research Center for Kyushu Okinawa Region (KONARC)
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KOBAYASHI Shuji
National Agricultural Research Center for Kyushu Okinawa Region
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SUDA Ikuo
National Agricultural Research Center for Kyushu Okinawa Region
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