Normal Calves Produced After Transfer of Embryos Cultured in a Chemically Defined Medium Supplemented with Epidermal Growth Factor and Insulin-like Growth Factor I Following Ovum Pick Up and In Vitro Fertilization in Japanese Black Cows

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概要

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• The objective of this study was to examine whether high concentrations of epidermal growth factor (EGF) and/or insulin-like growth factor I (IGF-I) would have a beneficial effect on bovine embryo development in vitro and to obtain normal calves by using an ovum pick up method and embryo culture in a chemically defined medium. When compared with controls, EGF (100 or 200 ng/ml) or IGF-I (50 or 100 ng/ml) significantly increased the rate of embryos that developed into blastocysts during an 8-day culture after the in vitro fertilization of oocytes obtained from ovaries from a slaughterhouse. IGF-I induced a dose-dependent increase in cell number in both the inner cell mass and the trophectoderm, whereas EGF stimulated proliferation only in the inner cell mass. A combination of EGF (100 ng/ml) and IGF-I (50 ng/ml) produced an additive effect, and embryos developed into blastocysts at a comparatively high rate (27.9%) compared with controls (12.0%). A similar rate of development was achieved using a combination of EGF and IGF-I in the culture of embryos following ovum pick up by ultrasound-guided transvaginal follicular aspiration and in vitro fertilization, and 5 blastocysts that developed after the culture were transferred into uteri; two embryos implanted, and normal calves were born. These results suggest that the combined use of EGF and IGF-I makes bovine embryo culture in a chemically defined medium a practical and useful procedure for producing blastocysts, and its application to embryo culture following ovum pick up and in vitro fertilization could be useful for producing normal calves.

著者

• KOBAYASHI Shuji

  National Livestock Breeding Center

• TAMADA Hiromichi

  Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefe

• 小林 修司

  (独)九州沖縄農業研究センター

• 小林 修司

  独立行政法人 家畜改良センター新冠牧場

• SAKAGAMI Nobutada

  Livestock Industry Technology Station, Kanagawa Agricultural Technology Center, Kanagawa 243-0417, J

• UMEKI Hidenobu

  Oita Prefectural Agriculture, Forestry and Fisheries Research Center Livestock Research Institute, O

• NISHINO Osamu

  Nara Prefectural Livestock Technology Center, Nara 633-1302, Japan

• UCHIYAMA Hiroko

  Miyazaki Prefectural Livestock Research Institute, Miyazaki 889-4411, Japan

• ICHIKAWA Kyoko

  Kochi Prefectural Livestock Research Institute, Kochi 789-1233, Japan

• TAKESHITA Kazuhisa

  Yamaguchi Prefectural Livestock Experiment Station, Yamaguchi 759-2221, Japan

• KANEKO Etsushi

  Livestock Industry Technology Station, Kanagawa Agricultural Technology Center, Kanagawa 243-0417, J

• AKIYAMA Kiyoshi

  Livestock Industry Technology Station, Kanagawa Agricultural Technology Center, Kanagawa 243-0417, J

• Tamada Hiromichi

  Department Of Advanced Pathobiology Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Sakagami Nobutada

  Livestock Industry Technology Station Kanagawa Agricultural Technology Center

• Nishino Osamu

  Nara Prefectural Livestock Technology Center

• Akiyama Kiyoshi

  Livestock Industry Technology Station Kanagawa Agricultural Technology Center

• Uchiyama Hiroko

  Miyazaki Prefectural Livestock Research Institute

• Umeki Hidenobu

  Oita Prefectural Agriculture Forestry And Fisheries Research Center Livestock Research Institute

• Ichikawa Kyoko

  Kochi Prefectural Livestock Research Institute

• Takeshita Kazuhisa

  Yamaguchi Prefectural Livestock Experiment Station

• Kaneko Etsushi

  Livestock Industry Technology Station Kanagawa Agricultural Technology Center

• AKIYAMA Kiyoshi

  Livestock Industry Technology Station, Kanagawa Agricultural Technology Center, Kanagawa 243-0417, Japan

• KOBAYASHI Shuji

  National Livestock Breeding Center, Fukushima 961-8511, Japan

• UMEKI Hidenobu

  Oita Prefectural Agriculture, Forestry and Fisheries Research Center Livestock Research Institute, Ooita 878-0201, Japan

• TAMADA Hiromichi

  Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan

• KANEKO Etsushi

  Livestock Industry Technology Station, Kanagawa Agricultural Technology Center, Kanagawa 243-0417, Japan

• KOBAYASHI Shuji

  National Agricultural Research Center for Kyushu Okinawa Region

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