Simple and Rapid Spectrophotometric Method for Selecting Purple-Fleshed Sweet Potato Cultivars with a High Radical-Scavenging Activity
スポンサーリンク
概要
- 論文の詳細を見る
Purple-fleshed sweet potato (Ipomoea batatas) plants containing anthocyanin pigments display a potent antioxidative activity. An 80 % ethanol extract from purple-fleshed sweet potato cultivars harvested in 2000 showed a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of 8.6 to 49.0 μmol expressed as Trolox equivalent/g of fresh weight. Although this DPPH radical-scavenging assay is convenient for the direct measurement of the radical-scavenging activity, a simpler and faster method should be developed for estimating the activity of a large number of breeding lines. For this purpose, the characterization of the UV-vis spectrum was performed in an 80 % ethanol extract from the purple-fleshed sweet potato. The extract exhibited absorption maxima near 325 nm and 530 nm due to the presence of cinnamic acids (or their related compounds) and anthocyanins, respectively. The absorbance at 325 nm was more highly correlated with the DPPH radical-scavenging activity than that at 530 nm and showed a correlation coefficient of 0.925. Multiple linear regression analysis using the absorbance of the extract at 325 nm and 530 nm elevated the value of the correlation coefficient to 0.979. When a prediction using this calibration curve was applied to extracts of purple-fleshed sweet potatoes harvested in 2001, a high correlation coefficient of 0.977 was obtained with lower SEP values of 1.686. Measurement of the absorbance of extracts from purple-fleshed sweet potatoes at 325 nm and 530 nm enable to obtain estimates of their DPPH radical-scavenging activity simply and rapidly. This assay could be suitable for selecting breeding lines of purple-fleshed sweet potato with a high DPPH radical-scavenging activity.
著者
-
Kobayashi Masatomo
BioResources Center
-
Oki Tomoyuki
National Agricultural Research Center For Kyushu Okinawa Region National Agricultural Research Organ
-
Suda Ikuo
National Agricultural Research Center For Kyushu Okinawa Region National Agricultural Research Organ
-
Suda Ikuo
Department Of Pharmacology Institute For Medical Immunology Kumamoto University Medical School
-
KUMAGAI Toru
Department of Molecular Medicine, Osaka University Graduate School of Medicine
-
OKI Tomoyuki
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
-
Sato Tetsuo
Graduate School Of Life Sciences Tohoku University
-
Kobayashi Mio
Department Of Crop And Food Science National Agricultural Research Center For Kyushu Okinawa Region
-
Kumagai Toru
Department Of Molecular Medicine Osaka University Graduate School Of Medicine
-
Kumagai Toru
Department Of Upland Farming Research National Agricultural Research Center For Kyushu Okinawa Regio
-
Nishiba Yoichi
Department Of Crop And Food Science National Agricultural Research Center For Kyushu Okinawa Region
-
Sato Tetsuo
Department of Chemistry, Tokyo Institute of Technology
-
Osame Miyuki
Department of Crop and Food Science, National Agricultural Research Center for Kyushu Okinawa Region
-
Masuda Mami
Department of Crop and Food Science, National Agricultural Research Center for Kyushu Okinawa Region
-
Furuta Shu
Department of Crop and Food Science, National Agricultural Research Center for Kyushu Okinawa Region
-
Furuta Shu
Department Of Crop And Food Science National Agricultural Research Center For Kyushu Okinawa Region
-
Masuda Mami
National Agricultural Research Center For Kyushu Okinawa Region National Agricultural Research Organ
-
Kobayashi Mio
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Fac
-
Osame Miyuki
Department Of Crop And Food Science National Agricultural Research Center For Kyushu Okinawa Region
-
Sato Tetsuo
Department Of Chemistry Tokyo Institute Of Technology
-
Kumagai Toru
Department Of Applied Chemistry Faculty Of Engineering Kumamoto University
-
Sato Tetsuo
Departmelet Of Organic Chemistry Tokyo Institute Of Technology Ookayama
関連論文
- Optical Microscopy and Scanning Electron Microscopy on the Surface of Rice Callus after Treatment with Cell Wall Degrading Enzymes
- Loss-of-function of a Rice Gibberellin Biosynthetic Gene, GA2O oxidase (GA20ox-2), Led to the Rice 'Green Revolution'
- CHARACTERIZATION OF RICE DWARF MUTANT, GIBBERELLIN INSENSITIVE DWARF1
- RICE DWARF MUTANT, d1,WHICH IS DEFECTIVE IN α SUBUNIT OF HETEROTRIMERIC G PROTEIN, AFFECTS IN GA SIGNAL TRANSDUCTION
- CHARACTERIZATION OF ANTHER SPECIFIC GIBBERELLIN 3β-HYDROXYLASE GENE, OsGA3oxl, FROM RICE (Oryza sativa L.)
- Ultrastructural Analysis of Electrofused Protoplasts from Pansy and Wild Viola by Scanning Electron Microscopy
- CHARACTERIZATION OF GIBBERELLIN 2β-HYDROXYLASE GENE FROM RICE (ORYZA SATIVAL.)
- Determination of Acylated Anthocyanin in Human Urine after Ingesting a Purple-Fleshed Sweet Potato Beverage with Various Contents of Anthocyanin by LC-ESI-MS/MS
- Estimated Average Daily Intake of Antioxidants from Typical Vegetables Consumed in Japan: A Preliminary Study
- Simple and Rapid Spectrophotometric Method for Selecting Purple-Fleshed Sweet Potato Cultivars with a High Radical-Scavenging Activity
- ISOLATION AND CHARACTERIZATION OF GIBBERELLIN INSENSITIVE SEVERE DWARF MUTANT IN RICE
- WT1 Peptide-Based Immunotherapy for Patients with Lung Cancer : Report of Two Cases
- Change in Catalytic Property of Trypsin Immobilized on Support Activated with Glutaraldehyde in Lower Protogenic Solvent
- Preparation of Glutaraldehyde Cross-linked Complex from Support
- Identification and characterization of genomic regions associated with nitrogen dynamics in rice plants (Oryza sativa L.)
- Quantitative Trait Loci Affecting Flag Leaf Development in Rice (Oryza sativa L.)
- ISOLATION AND CHARACTERIZATION OF TWO TYPES OF GA 3β-HYDROXYLASE GENES IN RICE
- SLENDER RICE, A CONSTITUTIVE GIBBERELLIN RESPONSE MUTANT
- Contracted form of endocardial fibroelastosis in two siblings
- D-1-2 Synthesis of 1,2-Diacylamino-1,2-dideoxy-β-D-glucopyranoses(Carbohydrates)
- Radical-scavenging Activities of Soybean Cultivars with Black Seed Coats
- Secretory component (polymeric immunoglobulin receptor) expression on human keratinocytes by stimulation with interferon-γ and differences in response
- Exon structure of the gene encoding the human mannose-binding protein-associated serine protease light chain: commparison with complement C1r and C1s genes
- Pulmonary Coccidioidomycosis That Formed a Fungus Ball with 8-years Duration
- Near-Infrared Reflectance Spectroscopic Analysis of Moisture, Fat, Protein, and Physiological Activity in Buckwheat Flour for Breeding Selection
- Evaluation of Antioxidant Activity of Vegetables from Okinawa Prefecture and Determination of Some Antioxidative Compounds
- Degradation of Alkylmercury to Inorganic Mercury by Oxygen Free Radical・OH (Proceedings of the 16 th Symposium on Environmental Pollutants and Toxicology)
- Contribution of β-Carotene to Radical Scavenging Capacity Varies among Orange-fleshed Sweet Potato Cultivars
- A New Method for Judging Deterioration of Stored Garden Peas, an Index of Lipid Peroxidation
- Fluorometric Determination of a 1,3-Diethyl-2-thiobarbituric Acid-Malondialdehyde Adduct as an Index of Lipid Peroxidation in Plant Materials
- Effects of Purple Sweet Potato Anthocyanins on Development and Intracellular Redox Status of Bovine Preimplantation Embryos Exposed to Heat Shock
- Luteolin, a Flavone, Does Not Suppress Postprandial Glucose Absorption Through an Inhibition of α-Glucosidase Action(Food & Nutrition Science)
- In Vitro Survey of α-Glucosidase Inhibitory Food Components
- Relationship between the Discharge Rate and the Maximum Hydrostatic Pressure upon Wet-Grinding of Soybean Using a Double-Disk Attrition Mill
- Classification of the Constituents of Citrus Juice Residue by a Wet-Grinding Process
- FORMATION AND CHARACTERIZATION OF THREE TYPES OF YEAST INVERTASE
- Analysis of PAS/LOV protein in Arabidopsis thaliana
- C3d and Epstein-Barr Virus (CR2/CD21 Ligands) Stimulate Cells of an HTLV-I Line, MT-2
- Determination of Free and Bound Phenolic Acids, and Evaluation of Antioxidant Activities and Total Polyphenolic Contents in Selected Pearled Barley
- D-8-1 Syntheses of Purine Nucleosides by the Fusion Method(Nucleosides)
- Effect of Surface Hydrophilicity of an Indium Oxide Electrode on Direct Electron Transfer of Myoglobins
- Palladium-catalyzed Direct C-H Bond Arylation of Simple Arenes with Aryltrimethylsilanes
- UV-Patterned Polysilane Films as a Phase Mask in Optical Correlation System
- Measurements of Acid-strength Distribution beyond H0=−8.2 of Silica-Alumina Catalysts by UV-Spectrophotometric n-Butylamine Titration Method
- Kinetic and NMR Spectroscopic Studies of the Fusion Reactions of Chloropurines with 1,2,3,5-Tetra-O-acetyl-D-ribofuranoses
- Extraction and Detection of mRNA from Horsehair
- The Involvement of Annexin II in Resistance to UVB-Induced Cell Death and in the Increased Nucleotide Excision Repair Capacity of UV-Damaged DNA in Human Cells