Extraction and Detection of mRNA from Horsehair
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概要
- 論文の詳細を見る
After RNA extraction from horsehair shafts and roots, the mRNAs of β-actin, muscle-type phosphofructokinase, and transforming growth factor-β1 were detected by reverse transcription polymerase chain reaction assay. Low amounts of RNA were present in the horsehair. These specific mRNA transcripts were readily detected when more than three hair roots were used. However, detection of the mRNA transcripts was difficult in the hair shaft. These findings indicate that the small amounts of residual RNA in horsehair roots can be utilized as samples for molecular biological analysis.
- 公益社団法人 日本獣医学会の論文
著者
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Itou Takuya
Department Of Preventive Veterinary Medicine And Animal Health Nihon University School Of Veterinary
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Sato Go
Department Of Biochemistry Hoshi University School Of Pharmacy And Pharmaceutical Sciences
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Sato Tetsuo
Departmelet Of Organic Chemistry Tokyo Institute Of Technology Ookayama
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Sakai Takeo
Department Of Pediatrics Tohoku University School Of Medicine
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SAKAI Takeo
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine
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SHOJI Youko
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine
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