Ultrastructural Analysis of Electrofused Protoplasts from Pansy and Wild Viola by Scanning Electron Microscopy

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概要

• 論文の詳細を見る

• Ultrastructural changes during the fusion of the protoplasts isolated from wild viola callus(without chloroplasts)and pansy mesophyll cells(with chloroplasts)were examined by scanning electron microscopy(SEM)and transmission electron microscopy(TEM). The presence of pansy protoplasts in the fusion products was proved by the presence of chloroplasts lying beneath the plasma membrane identified by SEM. Involvement of pansy chloroplasts in the fusion products was confirmed by TEM. Thus it was possible to distinguish between viola protoplasts and pansy protoplasts by SEM. During the process of protoplast fusion, adhesion of two protoplasts, the rupture and recovery of the membrane between them, and a single-paired spherical fusion body were clearly observed by SEM using our preparation method. Fused protoplasts divided and a microcolony was produced.

• 日本作物学会の論文

著者

• MIYAKE Hiroshi

  School of Marine Biosciences, Kitasato University

• Sato Tadahiko

  Graduate School of Bioagricultural Sciences, Nagoya University

• Taniguchi Takeshi

  Graduate School of Bioagricultural Sciences, Nagoya University

• Maeda Eizo

  Graduate School of Bioagricultural Sciences, Nagoya University

• Sato T

  Niigata Agricultural Res. Inst. Crop Res. Center Niigata Jpn

• Sato Tadahiko

  Environmental Engineering Division Nippon Sharyo Ltd.

• OH Chang

  Department of Materials Engineering, SungKyunKwan University

• Yong Mo

  Department of Horticulture, Don-A University

• Chung Han

  Department of Agricultural Chemistry, Don-A University

• Takeoka Yoji

  School of Agricultural Sciences, Nagoya University

• Taniguchi Takeshi

  School of Agricultural Sciences, Nagoya University

• Maeda Eizo

  Faculty of Bioindustry, Tokyo University of Agriculture

• Maeda E

  Nagoya Univ.

• Maeda E

  Graduate School Of Bioagricultural Sciences Nagoya University

• Maeda Eizo

  Nagoya Univ. Fac. Of Agriculture

• Oh Chang

  Department Of Biochemistry And Ilchun Molecular Medicine Institute Mrc Seoul National University Col

• Miyake H

  Nagoya Univ. Nagoya Jpn

• Takeoka Yoji

  Graduate School of Bioagricultural Sciences, Nagoya University

• Oh Chang

  Department Of Horticulture Don-a University

• Taniguchi Takeshi

  Graduate School Of Bioagricultural Sciences Nagoya University

• Taniguchi Takeshi

  The Graduate School Of Science And Technology Chiba University

• Taniguchi Takeshi

  Nagoya Univ. Fac. Of Agriculture

• Sato Tetsuo

  Graduate School Of Life Sciences Tohoku University

• Yong Mo

  Department Of Horticulture Don-a University

• Takeoka Y

  Nagoya Univ. Nagoya Jpn

• Takeoka Yoji

  School Of Agricultural Sciences Nagoya University

• Sato T

  Graduate School Of Bioagricultural Sciences Nagoya University

• Miyake Hiroshi

  School Of Marine Biosciences Kitasato Univ.

• Maeda Eizo

  Faculty Of Bioindustry Tokyo University Of Agriculture

• Chung Han

  Department Of Agricultural Chemistry Don-a University

• Miyake Hiroshi

  School Of Agricultural Sciences Nagoya University

• Miyake Hiroshi

  School of Agricultural Science, Nagoya University

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