Ultrastructure of Hybrid Callus between C_3 and C_4 Species of Amaranthaceae

概要

論文の詳細を見る
Unlike the parent line, the hybrid calli between C_3 and C_4 species of Amaranthaceae have poor division capability. Therefore, in this study the fine structures of hybrid callus derived from protoplasts of Celosia cristata L. cv. Pink Charm (C_3 species) cell suspension and Amaranthus tricolor L. cv. Perfecta (C_4 species) cotyledon callus were investigated by light and electron microscopy, and compared with the parental. All callus lines were composed of parenchymatous cells possessing a voluminous vacuole. Parental callus lines contained organelles with a relatively normal structure. The peculiar feature characterizing the C_3/C_4 hybrid callus was the presence of highly lobed nucleus with multinucleoli and numerous small vacuoles having autophagic activity scattered in the cytoplasm. The autophagic activity is apparently related to the existence of several inclusions such as cytoplasmic organelles in the central vacuole. It might also be related to the pronounced expansion of central vacuole and the reduced cytoplasm. The failure to sustain proliferation related to some atypical features of the organelles is discussed.
日本作物学会の論文