Interspecies Nuclear Transfer Embryos Reconstructed from Cat Somatic Cells and Bovine Ooplasm
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概要
- 論文の詳細を見る
This study was conducted to investigate the developmental capacity of domestic cat-bovine reconstructed embryos via interspecies somatic cell nuclear transfer (iSCNT) and to observe the mitochondrial DNA (mtDNA) content of the iSCNT embryos. The iSCNT embryos were generated using mixed-breed domestic cat fibroblasts as donor cells and enucleated bovine oocytes as the recipient cytoplasm. When the developmental capacities of iSCNT embryos and parthenogenic bovine embryos were compared, there was no difference (P>0.05) in the rates of cleavage and development to the 8-cell stage (86.6% vs. 84.0% and 32.2% vs. 36.2%, respectively). However, in contrast to development of parthenogenic embryos to the morula and blastocyst stages, no iSCNT embryos (0/202) developed beyond the 8-cell stage. For mtDNA analysis, iSCNT embryos at the 1-cell, 2-cell, 4-cell and 8-cell stages were randomly selected. Both cat and bovine mtDNA quantification analysis were performed using quantitative PCR. The levels of both cat and bovine mtDNA in cat-bovine iSCNT embryos varied at each stage of development. The cat mtDNA concentration in the iSCNT embryos was stable from the 1-cell to 8-cell stages. The bovine mtDNA in the iSCNT embryos at the 8-cell stage was significantly lower than that at the 4-cell stage (P<0.05). No difference in the proportions of cat mtDNA in the iSCNT embryos was found in any of the observed developmental stages (1- through 8-cell stages). In conclusion, bovine cytoplasm supports domestic cat nucleus development through the 8-cell stage. The mtDNA genotype of domestic cat-bovine iSCNT embryos illustrates persistence of heteroplasmy, and the reduction in mtDNA content might reflect a developmental block at the 8-cell stage.
- 日本繁殖生物学会の論文
- 2008-04-01
著者
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TAGAMI Takahiro
National Institute of Livestock and Grassland Science
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Inaba Yasushi
National Livestock Breeding Center
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IMAI Kei
National Livestock Breeding Center
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Nagai Takashi
東京農工大学 大学院農学府
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Nagai Takashi
Department of Internal Medicine, Public Tomioka General Hospital
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TECHAKUMPHU Mongkol
Department of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn
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Nagai Takashi
Dep. Of Animal Breeding And Reproduction National Inst. Of Livestock And Grassland Sci.
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TAKEDA Kumiko
National Institute of Livestock and Grassland Science
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Nagai Takashi
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Nagai T
National Inst. Agrobiological Sci. Ibaraki Jpn
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Kobayashi Shu-ichi
National Livestock Breeding Center
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Techakumphu Mongkol
Department Of Obstetrics Gynaecology And Reproduction Faculty Of Veterinary Science Chulalongkorn Un
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Techakumphu Mongkol
Department Of Obstetrics Gynecology And Reproduction Faculty Of Veterinary Science Chulalongkorn Uni
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THONGPHAKDEE Ampika
Department of Obstetrics, Gynecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn
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KOBAYASHI Shuji
Embryo Transfer and In Vitro Fertilization Section, National Livestock Breeding Center
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IMAI Kei
Embryo Transfer and In Vitro Fertilization Section, National Livestock Breeding Center
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INABA Yasushi
Embryo Transfer and In Vitro Fertilization Section, National Livestock Breeding Center
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TASAI Mariko
Department of Animal Breeding and Reproductive, National Institute of Livestock and Grassland Scienc
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TAGAMI Takahiro
Department of Animal Breeding and Reproductive, National Institute of Livestock and Grassland Scienc
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NIRASAWA Keijiro
Department of Animal Breeding and Reproductive, National Institute of Livestock and Grassland Scienc
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SAITO Norio
Embryo Transfer and In Vitro Fertilization Section, National Livestock Breeding Center
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TAKEDA Kumiko
Department of Animal Breeding and Reproductive, National Institute of Livestock and Grassland Scienc
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Tasai Mariko
Department Of Animal Breeding And Reproductive National Institute Of Livestock And Grassland Science
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Tagami T
Division Of Earth And Planetary Sciences Graduate School Of Science Kyoto University
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Tagami Takahiro
Department Of Animal Breeding And Reproductive National Institute Of Livestock And Grassland Science
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Tatsumi Kouichi
Fac. Of Agriculture Shinshu Univ. Jpn
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Takeda Kumiko
Fac. Of Agriculture Shinshu Univ. Jpn
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Nagai Takuya
Nasu Institute Nisshin Flour Milling Co. Ltd.
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Nagai Takashi
National Inst. Of Livestock And Grassland Sci.
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Thongphakdee Ampika
Department Of Obstetrics Gynecology And Reproduction Faculty Of Veterinary Science Chulalongkorn Uni
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Saito Norio
Embryo Transfer And In Vitro Fertilization Section National Livestock Breeding Center
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Nirasawa Keijiro
National Agricultural And Food Res. Organization (naro) Inst. Of Livestock And Grassland Sci. (nilgs) Ibaraki ...
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