豚卵母細胞のDNA損傷と体外発育に及ぼす卵巣の保存時間と温度の影響
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概要
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The present study was conducted to investigate the effects of storage time and temperature of porcine ovaries on the quality and nuclear maturation in vitro of oocytes obtained from stored ovaries and their subsequent development after in vitro fertilization. The ovaries were stored in physiological saline for 0, 3, 6, 9 and 12 h at various temperatures (4, 15, 25 and 35 C). The pH of follicular fluid obtained from the ovaries, DNA fragmentation of the oocyte nucleus and meiotic competence of oocytes were examined. Some oocytes from ovaries stored at 15, 25 and 35 C for 6 h were fertilized in vitro, and then cultured for 7 days to examine the ability of embryos to develop to the blastocyst stage. When the ovaries were stored at 35 C, the pH of follicular fluid decreased and the proportions of oocytes with DNA fragmented nuclei increased as the storage time was prolonged, and the storage of ovaries for 6, 9 and 12 h resulted in lower maturation rates of oocytes. When the ovaries were stored at 4, 15, 25 and 35 C for 6 h, the storage at higher temperatures (≥15 C) decreased the pH of follicular fluid and induced nucleic DNA fragmentation in higher proportions of oocytes. None of the oocytes from ovaries stored at 4 C reached metaphase II. The storage of ovaries at 15 C reduced the rates of in vitro fertilized oocytes and subsequent embryo development, but there were no significant differences in the rates of fertilization and blastocyst formation between oocytes from ovaries stored at 25 C and 35 C. Our findings indicate that the storage of ovaries at 25-35 C for 6 h is effective for maintaining the developmental competence of porcine oocytes even though the development rates were lower than those of ovaries stored at 35 C for 3 h.
- 日本繁殖生物学会の論文
- 2005-02-01
著者
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音井 威重
山口大学大学院連合獣医学研究科
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Nagai Takashi
Dep. Of Animal Breeding And Reproduction National Inst. Of Livestock And Grassland Sci.
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永井 卓
(独)・農業・生物系特定産業技術研究機構・畜産草地研究所
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音井 威重
山口大学農学部
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永井 卓
独立行政法人 農業・食品産業技術総合研究機構 畜産草地研究所
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WONGSRIKEAO Pimprapar
山口大学大学院連合獣医学研究科
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KARJA Ni
山口大学大学院連合獣医学研究科
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AGUNG Budiyanto
山口大学大学院連合獣医学研究科
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新居 雅宏
徳島県立農林水産総合技術センター・肉畜分場
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Otoi Takeshige
Lab. Of Animal Reproduction The United Graduate School Of Veterinary Sci. Yamaguchi Univ.
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Agung Budiyanto
Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers
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Wongsrikeao Pimprapar
Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers
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Nagai T
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Karja Ni
Laboratory Of Animal Reproduction United Graduate School Of Veterinary Science Yamaguchi University
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Nagai Takuya
Nasu Institute Nisshin Flour Milling Co. Ltd.
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Nagai Takashi
National Inst. Of Livestock And Grassland Sci.
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