<I>In Vitro</I> Development of <I>in Vivo</I> and <I>in Vitro</I> Fertilized Porcine Zygotes
スポンサーリンク
概要
- 論文の詳細を見る
To identify an <I>in vitro</I> culture procedure which can be used to assess porcine <I>in vitro</I> fertilized embryos, three culture systems were examined. A total of 103 <I>in vivo</I> fertilized zygotes were co-cultured in modified BMOC-3 medium with granulosa cells, or in modified BMOC-2 with oviduct cells, and 35 were cultured in Whittens medium supplemented with 1.5% BSA. After 8 days in culture, 66.0 and 73.6% of the co-cultured zygotes, and 51.4% of the zygotes cultured in the modified Whittens medium developed to blastocyst stage. In contrast, only 20.5% (18/88) and 1.4% (9/627) of <I>in vitro</I> fertilized zygotes derived from <I>in vivo</I> and <I>in vitro</I> matured oocytes developed to blastocysts in the co-culture with modified BMOC-3 and granulosa cells. It is concluded that the culture systems examined are competent to support development of porcine zygotes to blastocysts and evaluate <I>in vitro</I> fertilized porcine embryos.
- 日本繁殖生物学会の論文
著者
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永井 卓
(独)・農業・生物系特定産業技術研究機構・畜産草地研究所
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Nagashima Hiroshi
Laboratory Of Reproduction Engineering Meiji University
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Nagashima H
Meiji Univ. Graduate School Kanagawa
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Nagai T
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Yamakawa Hirohito
Nasu Institute, Nisshin Flour Milling Co., Ltd., 1242 Iguchi, Nishinasuno, Nasu, Tochigi 329-2763, J
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Nagai Takuya
Nasu Institute, Nisshin Flour Milling Co., Ltd., 1242 Iguchi, Nishinasuno, Nasu, Tochigi 329-2763, J
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Nagashima Hiroshi
Nasu Institute, Nisshin Flour Milling Co., Ltd., 1242 Iguchi, Nishinasuno, Nasu, Tochigi 329-2763, J
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Nagai Takuya
Nasu Institute Nisshin Flour Milling Co. Ltd.
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Yamakawa Hirohito
Nasu Institute Nisshin Flour Milling Co. Ltd.
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NAGASHIMA Hiroshi
Nasu Institute, Nisshin Flour Milling Co., Ltd.
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NAGAI Takuya
Nasu Institute, Nisshin Flour Milling Co., Ltd.
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