成熟培養時間がウシ胚盤胞期胚の性比におよぼす影響

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概要

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• It has been suggested that the maturational stage of oocytes at time of insemination influences the sex ratio of resulting embryos. However, there are very few reports concerning the relationship between the maturation culture period of oocytes and the sex ratio of resulting embryos. The objective of this study was to investigate the effects of in vitro maturation culture period for bovine oocytes on the sex ratio of in vitro produced blastocysts using a novel technique of loop-mediated isothermal amplification (LAMP). Cumulus-oocyte complexes were collected from the ovaries of slaughtered cows, and then matured in vitro for various periods (16, 22, 28, and 34 h). After maturation culture for each period, the oocytes were inseminated with frozen-thawed spermatozoa, and then cultured in vitro. Blastocysts were harvested on Day 7 after insemination, and the sex of the embryos was examined using the LAMP method. The rates of oocytes matured to the metaphase II stage were significantly lower (P<0.05) in the 16-h maturation group than in the other groups. The proportion of blastocyst formation after insemination was significantly higher (P<0.05) in the 22-h maturation group than in the other groups. The proportion of male blastocysts increased with the increase in maturation culture period. The proportion of male blastocysts derived from oocytes matured for 34 h was significantly higher (P<0.05) than from oocytes matured for 16 and 22 h. These results indicate that the sex ratio of in vitro fertilized embryos is apparently influenced by the maturation culture period of the oocytes

• 日本繁殖生物学会の論文
• 2006-02-01

著者

• Nagai Takashi

  東京農工大学 大学院農学府

• 音井 威重

  山口大学大学院連合獣医学研究科

• Shimizu Ryohei

  Lab. Of Animal Reproduction United Graduate School Of Veterinary Sci. Yamaguchi Univ.

• Nagai Takashi

  Dep. Of Animal Breeding And Reproduction National Inst. Of Livestock And Grassland Sci.

• 永井 卓

  (独)・農業・生物系特定産業技術研究機構・畜産草地研究所

• 音井 威重

  山口大学農学部

• 永井 卓

  独立行政法人 農業・食品産業技術総合研究機構 畜産草地研究所

• AGUNG Budiyanto

  山口大学農学部獣医学科

• WONGSRIKEAO Pimprapar

  山口大学農学部獣医学科

• 谷口 雅康

  山口大学農学部獣医学科

• 清水 良平

  山口大学農学部獣医学科

• 渡 一

  (株)栄研化学

• Otoi Takeshige

  Lab. Of Animal Reproduction The United Graduate School Of Veterinary Sci. Yamaguchi Univ.

• Nagai T

  National Inst. Agrobiological Sci. Ibaraki Jpn

• Agung Budiyanto

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• Taniguchi Masayasu

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• 谷口 雅康

  山口大学農学部

• Wongsrikeao Pimprapar

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• Nagai T

  Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science

• Shimizu Ryohei

  Laboratory Of Animal Reproduction United Graduate School Of Veterinary Science Yamaguchi University

• Nagai Takuya

  Nasu Institute Nisshin Flour Milling Co. Ltd.

• Nagai Takashi

  National Inst. Of Livestock And Grassland Sci.

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