A Comparison of Cryotop and Solid Surface Vitrification Methods for the Cryopreservation of In Vitro Matured Bovine Oocytes
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概要
- 論文の詳細を見る
The aim of the present study was to compare the efficacies of the cooling systems of the solid surface (SSV) and Cryotop vitrification methods for cryopreservation of bovine oocytes at the metaphase II stage. The effects of vitrification on oocyte viability, in vitro fertilization (IVF), pronucleus formation and subsequent in vitro development were assessed. In vitro matured (IVM) bovine oocytes were subjected to equilibration and vitrification solutions according to the SSV method, and then the oocytes were vitrified either by dropping onto a cold dry metal surface (SSV group) or by plunging into liquid nitrogen on Cryotop sheets (Cryotop group). Warming was conducted according to the SSV method. Some oocytes were subjected to the cryoprotectants and warming regimen without cooling (Solution control group). The live/dead status of oocytes was evaluated by fluorescein diacetate staining. Live oocytes were subjected to IVF, and the resultant embryos were cultured in vitro. The rates of live oocytes were similar among the Fresh control, Solution control, SSV and Cryotop groups. There was no difference in the rates of fertilization, pronuclear formation and monospermy among these groups. The cleavage rates in the SSV and Cryotop groups (41.6 and 53.2%, respectively) were significantly lower than those in the Fresh control and Solution control groups (65.9 and 61.3%, respectively). The blastocyst rates in SSV and Cryotop groups did not differ (10.3 and 12.8%, respectively); however, they were significantly lower than those in the Fresh control and Solution control groups (36.4 and 24.8%, respectively). The inner cell mass, trophectoderm and total cell numbers in blastocysts did not differ significantly among the Fresh control, Solution control, SSV and Cryotop groups. Our results indicate that IVM bovine oocytes could be cryopreserved successfully using the cooling systems of the Cryotop and Solid Surface Vitrification methods with similar efficacy.
- 日本繁殖生物学会の論文
著者
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Somfai Tamas
National Livestock Breeding Center
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Inaba Yasushi
National Livestock Breeding Center
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IMAI Kei
National Livestock Breeding Center
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NAGAI Takashi
National Institute of Livestock and Grassland Science
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Imai Kei
National Inst. Agrobiological Sci. Ibaraki Jpn
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PARNPAI Rangsun
Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te
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SRIPUNYA Nucharin
Embryo Technology and Stem Cell Research Center, Suranaree University of Technology
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Parnpai Rangsun
Embryo Technol. And Stem Cell Res. Center School Of Biotechnology Suranaree Univ. Of Technol.
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Nagai Takashi
National Inst. Of Livestock And Grassland Sci.
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Sripunya Nucharin
Embryo Technology And Stem Cell Research Center Suranaree University Of Technology
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Somfai Tamas
National Institute Of Livestock And Grassland Science
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INABA Yasushi
National Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Ibaraki 305-0901, Japan
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