Effect of Cryoprotectant Composition on In Vitro Viability of In Vitro Fertilized and Cloned Bovine Embryos Following Vitrification and In-Straw Dilution

スポンサーリンク

概要

• 論文の詳細を見る

• In this study the efficacy of the combination of glycerol (GLY) and ethylene glycol (EG) as cryoprotectants in a vitrification method developed for direct embryo transfer was evaluated by in vitro development of in vitro fertilized (IVF) and somatic cell nuclear transfer (SCNT) embryos after vitrification. The IVF and SCNT blastocysts were vitrified in either 40% GLY, 30% GLY + 10% EG, or 20% GLY + 20% EG using French straws. After warming, the straws were held vertically for 1 min without shaking and were then placed horizontally for 5 min to dilute the cryoprotectants. After washing, the vitrified-warmed embryos were cultured in vitro for 72 h. There were no differences among the vitrification solutions with respect to the rates of vitrified-warmed IVF and SCNT embryos surviving and developing to the hatched blastocyst stage. However, the rates of development to the hatched blastocyst stage of the SCNT embryos vitrified with 40% GLY tended to be higher than those vitrified with 30% GLY + 10% EG or 20% GLY + 20% EG (26% vs. 7-8%, respectively). The development rates to the hatched blastocyst stage of the IVF and SCNT embryos vitrified with solution containing EG were significantly lower (P<0.05) than those of non-vitrified embryos. These results suggest that use of the combination of GLY and EG as cryoprotectants had no beneficial effect on the viability of embryos after in-straw dilution. However, this method is so simple that it can be used for practical direct transfer of vitrified embryos in the field.

• 日本繁殖生物学会の論文
• 2007-08-01

著者

• Nagai Takashi

  東京農工大学 大学院農学府

• 音井 威重

  山口大学大学院連合獣医学研究科

• Nagai Takashi

  Department of Internal Medicine, Public Tomioka General Hospital

• Nagai Takashi

  Dep. Of Animal Breeding And Reproduction National Inst. Of Livestock And Grassland Sci.

• 永井 卓

  (独)・農業・生物系特定産業技術研究機構・畜産草地研究所

• TANIGUCHI Masayasu

  Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi Unive

• IKEDA Akiko

  Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi Unive

• ARIKAWA Eri

  Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi Unive

• WONGSRIKEAO Pimprapar

  Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi Unive

• AGUNG Budiyanto

  Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi Unive

• NAOI Hideaki

  Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi Unive

• OTOI Takeshige

  Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi Unive

• 音井 威重

  山口大学農学部

• 永井 卓

  独立行政法人 農業・食品産業技術総合研究機構 畜産草地研究所

• 谷口 雅康

  山口大学農学部獣医学科

• Naoi Hideaki

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• Arikawa Eri

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• Otoi Takeshige

  Lab. Of Animal Reproduction The United Graduate School Of Veterinary Sci. Yamaguchi Univ.

• Otoi Takeshige

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• Nagai Takashi

  Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science

• Nagai T

  National Inst. Agrobiological Sci. Ibaraki Jpn

• Agung Budiyanto

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• Taniguchi Masayasu

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• Wongsrikeao Pimprapar

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• Ikeda Akiko

  Laboratory Of Animal Reproduction The United Graduate School Of Veterinary Science Yamaguchi Univers

• Nagai T

  Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science

• Nagai Takuya

  Nasu Institute Nisshin Flour Milling Co. Ltd.

• Nagai Takashi

  National Inst. Of Livestock And Grassland Sci.

関連論文

• Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
• ヒストン脱アセチル化酵素の阻害剤トリコスタチンAは卵丘細胞に包まれたGV期ブタ卵母細胞のクロマチンを脱凝縮状態に逆戻りさせる
• 受胚牛の品種および人工初乳の給与が胚移植により生産された黒毛和種雄子牛の発育および血液成分に及ぼす影響
• ウマヘルペスウイルス分離・増殖のためのウマ由来細胞株の樹立(短報)(ウイルス学)
• Reactive Hypoglycemia Associated with Transient Hyperthyroidism in the Background of the Prediabetic Stage
• 沖縄島漫湖饒波川のマングローブ域における大型底生生物の分布と出現量
• Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
• Effect of Cryoprotectant Composition on In Vitro Viability of In Vitro Fertilized and Cloned Bovine Embryos Following Vitrification and In-Straw Dilution
• ビーグル犬の心エコー法における形態・機能学的な標準値
• 成熟培養時間がウシ胚盤胞期胚の性比におよぼす影響
• 豚卵母細胞のDNA損傷と体外発育に及ぼす卵巣の保存時間と温度の影響
• ウシ卵巣の前胞状卵胞および胞状卵胞における卵胞と卵母細胞の成長に関する対比成長測定学的研究
• 電気刺激およびブチロラクトンIを用いた活性化刺激による体外成熟豚卵子の活性化およびその後の胚発生
• ウシ卵巣の前胞状卵胞および胞状卵砲における卵胞と卵母細胞の成長に関する対比成長測定学的研究
• ウシ卵子における卵核胞崩壊に対するブチロラクトン-Iの影響とその後の卵子成熟、受精および発生能
• 越壁IVF法による精子選別は豚卵子への多精子受精頻度を減少させる
• 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
• 豚における体外受精とくに体外成熟・受精卵子の雄性前核形成に関する研究(1993年度佐藤賞受賞講演論文)
• わが国における体細胞クローン牛を対象とした健全性調査の実施状況
• ウシ雄性発生胚の作出と体外発生
• 序
• 黄体ホルモン製剤を膣内に留置した黒毛和種牛への反復過剰排卵処置における GnRH 投与の効果
• Deterioration of Retinopathy After Starting Interferon Therapy for Chronic Hepatitis C with Diabetic Triopathy
• Secondary Hemochromatosis Accompanied by Diabetes Mellitus and Hypogonadism.
• Seroconversion of Glutamic Acid Decarboxylase Antibodies in a Patient Initially Diagnosed as Having Type 2 Diabetes Mellitus
• Metformin Use in an Obese Diabetic Patient from Weeks 1 to 21 of Pregnancy.
• Sarcoid Granulomatous Interstitial Nephritis Accompanied by Type 2 Diabetes Mellitus
• Graves' Disease Accompanied by Anti-myeloperoxidase Antibody-related Nephropathy and Autoimmune Hepatitis
• 17α-Hydroxylase Deficiency Accompanied by Adrenal Myelolipoma
• Effect of Donor Cell Types on Developmental Potential of Cattle (Bos taurus) and Swamp Buffalo (Bubalus bubalis) Cloned Embryos
• Effects of Season and Reproductive Phase on the Quality, Quantity and Developmental Competence of Oocytes Aspirated from Japanese Black Cows
• 黒毛和種牛における分娩後の発情誘起が子宮修復と胚回収およびその後の受胎成績に及ぼす影響
• Developmental Competence of Cat Oocytes from Ovaries Stored at Various Temperature for 24 h
• 異なる光学異性体から構成されるPGF_製剤が過剰排卵処置後の採胚成績および発情回帰に及ぼす影響
• ネコ卵母細胞のガラス化保存と体外発育に及ぼすグルコースの影響
• 凍結融解組織由来体細胞によって構築された牛核移植胚の発生比較(短報)
• 牛卵母細胞の体外成熟,受精と発生に係るアラニン,グリシンと成長ホルモンとの関係
• 薄切法により卵巣から採取した犬卵母細胞の品質と成熟能(短報)
• 牛単為生殖卵由来核の発育能の評価
• 簡易炭酸ガス培養器を用いた牛体外受精胚の作出 : 陰圧気相条件が胚の発生におよぼす影響
• 黒毛和種牛における最大卵胞除去もしくはhCG前処置後の過剰排卵反応(短報)
• 正常に発育し妊娠した凍結融解牛体外成熟卵子由来子牛の1例
• K99保有大腸菌の牛胚への曝露と洗浄, 抗生物質処理およびトリプシン処理による除去効果(短報)
• トリプシン処理が牛体外受精胚の生存および発育に与える影響
• 牛の体外受精胚に付着した細菌に対する抗生物質処理効果
• シクロヘキシミドへの曝露が電気的に活性化したネコ卵母細胞の発育に及ぼす効果
• Birth of Cloned Calves Derived from Cultured Oviductal Epithelial Cells of a Dairy Cow
• Birth of Cloned Calves Derived from Cultured Oviductal Epithelial Cells of a Dairy Cow
• Taxonomy of the Terrestrial Crab Geothelphusa tenuimanus (Crustacea: Decapoda: Brachyura: Potamidae) of Okinawa Island, Central Ryukyus, Japan
• 培養卵丘細胞をドナー核とした体外成熟ブタ卵母細胞の核移植後の発生能力
• Effect of Validamycin on β-d-Glucan-degrading Enzymes from Rhizoctonia solani
• 修正合成卵管液(m-SOF)に添加した乳酸とグルコースが牛体外受精胚の発生に及ぼす効果
• Evaluation of Developmental Competence of In Vitro-produced Porcine Embryos Based on the Timing, Pattern and Evenness of the First Cleavage and Onset of the Second Cleavage
• Intracytoplasmic Sperm Injection in the Pig : Where is the Problem?
• Nuclear and Cytoplasmic Maturation In Vitro of Porcine Oocytes
• Effects of Cysteine During In Vitro Maturation of Porcine Oocytes Under Low Oxygen Tension on Their Subsequent In Vitro Fertilization and Development
• Effect of Bezafibrate or Pravastain on Serum Lipid Levels and Albuminuria in MIDDM Patients
• Effect of Nephropathy on the Composition of Apolipoprotein-Containing Particles in NIDDM
• Prader-Willi Syndrome with Elevated Follicle Stimulating Hormone Levels and Diabetes Mellitus
• Diabetes Mellitus Accompanied by Nonocclusive Colonic Ischemia
• Candida Glabrata Fungemia After Amputation of an Infected Diabetic Foot
• Cervical Spinal Epidural Abscess and Perirenal Abscess Associated with Diabetes Mellitus
• An Overwhelming Postsplenectomy Infection Syndrome Associated with Diabetes M ellitus
• M ultiple Organ Failure Followed by Intrauterine Klebsiella Pneumoniae Infection Associated with Diabetes M ellitus
• An approach for conservation of pig resources by xenograft of gonadal tissue to mice
• Effect of Centrifugation Treatment before Vitrification on the Viability of Porcine Mature Oocytes and Zygotes Produced In Vitro
• 初期胞状卵胞から採取した牛卵母細胞の生存と体外成長に及ぼす酸素濃度の影響
• A Comparison of Cryotop and Solid Surface Vitrification Methods for the Cryopreservation of In Vitro Matured Bovine Oocytes
• Germinal Vesicle Stages in Pig Follicular Oocytes Collected by Different Methods
• 体外生産ブタ胚の体外培養における培地中のピルビン酸塩/乳酸塩のグルコースへの置換時期が胚発生におよぼす影響
• Interspecies Nuclear Transfer Embryos Reconstructed from Cat Somatic Cells and Bovine Ooplasm
• Larval Development of a Pontoniine Shrimp, Periclimenes brevicarpalis (Crustacea : Decapoda : Palaemonidae) Reared in the Laboratory
• 無血清成熟培地へのシステアミンの添加がウシ未成熟卵子の体外成熟率,体外受精率およびその後の発生率に及ぼす影響
• Pregnancy complicated with Alport syndrome : A good obstetric outcome and failure to diagnose an infant born to a mother with Alport syndrome by umbilical cord immunofluorescence staining
• Developmental Competence of Cat Oocytes from Ovaries Stored at Various Temperature for 24h
• Effect of Cycloheximide on In Vitro Development of Electrically Activated Feline Oocytes
• Effect of Replacement of Pyruvate/Lactate in Culture Medium with Glucose on Preimplantation Development of Porcine Embryos In Vitro
• Development of Porcine Embryos Reconstructed by Nuclear Transfer of Cultured Cumulus Cells into In Vitro Matured and Enucleated Oocytes
• Ejaculated Boar Spermatozoa Can Penetrate Oocytes Maturing In Vitro without Preincubation
• Solids Circulation Rate in Screen-Bottomed Spouted Bed with Draft-Tube
• Nitrogen Uptake by Paddy Rice (Oryza sativa L.) from ^N Labelled Coated Urea and Ammonium Sulfate
• Growth, Prey Consumption and Food Assimilation Efficiency in a Mantid, Paratenodera angustipennis (S.)
• Germinal Vesicle Stages in Pig Follicular Oocytes Collected by Different Methods
• Production of Transgenic Pig Carrying MMTV/v-Ha-ras
• In Vitro Development of in Vivo and in Vitro Fertilized Porcine Zygotes
• 由来の確かなウシ卵子の超低温保存技術による子牛の生産, 国内で初めて成功
• Evaluation of Developmental Competence of In Vitro-produced Porcine Embryos Based on the Timing, Pattern and Evenness of the First Cleavage and Onset of the Second Cleavage
• 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
• Vitrification of Fully Grown and Growing Porcine Oocytes Using Germinal Vesicle Transfer
• The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos
• Cloned Cows with Short Telomeres Deliver Healthy Offspring with Normal-length Telomeres
• Relationships of Survival Time, Productivity and Cause of Death with Telomere Lengths of Cows Produced by Somatic Cell Nuclear Transfer
• Improvement of Porcine Oocytes with Low Developmental Ability after Fusion of Cytoplasmic Fragments Prepared by Serial Centrifugation
• Subclinical Cushing's Disease Accompanied by Malignant Hypertension and Diabetes Mellitus
• The Effect of Ovary Storage and In Vitro Maturation on mRNA Levels in Bovine Oocytes : A Possible Impact of Maternal ATP1A1 on Blastocyst Development in Slaughterhouse-derived Oocytes
• In vitro production of porcine embryos : current status, future perspectives and alternative applications
• Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm
• Factors Affecting Cryopreservation of Porcine Oocytes
• Influence of Intergeneric/Interspecies Mitochondrial Injection : Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells
• 序

もっと見る 閉じる

スポンサーリンク