Evaluation of Developmental Competence of In Vitro-produced Porcine Embryos Based on the Timing, Pattern and Evenness of the First Cleavage and Onset of the Second Cleavage
スポンサーリンク
概要
- 論文の詳細を見る
The following selection markers for in vitro-produced porcine embryos were investigated: the timing, pattern and evenness of the first cleavage and the timing of the second cleavage. The embryos that cleaved by 30 h post-insemination (hpi) developed to blastocysts at a significantly higher rate (60.9%) and with a significantly higher cell number (33.6 cells) than those of embryos cleaved by 36 hpi (26.4% and 23.6 cells, respectively, P<0.05). Blastocyst proportions derived from 2- and 3-cell embryos cleaved by 30 hpi (68.2 and 65.3%, respectively) were significantly higher than those of 4- and >4-cell embryos (46.3 and 42.6%, respectively, P<0.05). The cell number per blastocyst generated from 2-cell embryos was significantly greater (37.3 cells) than those from 3-, 4- and >4-cell embryos (23.6-27.8 cells, P<0.05). Among embryos cleaved by 30 hpi, the blastocysts derived from evenly cleaved embryos (40.6 cells) were of significantly better quality than those derived from unevenly cleaved embryos (33.2 cells, P<0.05), although their blastocyst rates did not differ. The evenly cleaved embryos that underwent subsequent cleavage within 18 h had significantly higher blastocyst rates (72.7-81.0%) and quality (36.2-40.9 cells) than those without subsequent cleavage (48.3% and 22.5 cells, respectively, P<0.05) during the same period. In conclusion, the timing, pattern and evenness of the first cleavage and the timing of the second cleavage affected the developmental competence and quality of in vitro-produced porcine embryos.
- 日本繁殖生物学会の論文
著者
-
KANAI Yukio
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
Kikuchi Kazuhiro
Department Of Internal Medicine School Of Medicine Tokai University
-
Kikuchi Kazuhiro
東京農工大学 大学院農学府
-
Nagai Takashi
Department of Internal Medicine, Public Tomioka General Hospital
-
Dang-nguyen Thanh
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
-
金井 幸雄
筑波大学農林学系
-
Kikuchi Kazuhiro
Dep. Of Animal Sci. National Inst. Of Agrobiological Sciences
-
OZAWA Manabu
Department of Animal Science, National Institute of Agrobiological Sciences
-
Somfai Tamas
Division Of Animal Sciences National Institute Of Agrobiological Sciences
-
Kanai Yukio
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Ozawa Manabu
Department Of Animal Science National Institute Of Agrobiological Sciences
-
Kikuchi Kazuhiro
Department Of Animal Science National Institute Of Agrobiological Sciences
-
Somfai Tamas
Department of Animal Science, National Institute of Agrobiological Sciences
-
NAKAI Michiko
Department of Animal Science, National Institute of Agrobiological Sciences
-
MAEDOMARI Naoki
Department of Animal Science, National Institute of Agrobiological Sciences
-
VIET-LINH Nguyen
Department of Animal Science, National Institute of Agrobiological Sciences
-
NGUYEN Bui
Laboratory of Embryo Technology, Institute of Biotechnology, Vietnamese Academy of Science and Techn
-
Nagai Takashi
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
-
金井 幸雄
筑波大学大学院生命環境科学研究科
-
Viet-linh Nguyen
Department Of Animal Science National Institute Of Agrobiological Sciences
-
Somfai Tamas
Department Of Animal Science National Institute Of Agrobiological Sciences
-
Kanai Yukio
Laboratory Of Animal Science Institute Of Agriculture And Forestry University Of Tsukuba
-
DANG-NGUYEN Thanh
Department of Animal Science, National Institute of Agrobiological Sciences
-
SOMFAI Tamas
Department of Animal Breeding and Reproduction, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
-
NGUYEN Bui
Laboratory of Embryo Technology, Institute of Biotechnology, Vietnamese Academy of Science and Technology
関連論文
- Migratory Ability of Chick Primordial Germ Cells Transferred into Quail Embryos
- Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
- A Case of Spontaneous Esophageal Rupture Treated with Conservative Therapy
- Endoscopic Manometry of the Sphincter of Oddi in Dogs Pharmacological Effects of Anticholinergic and Selective Antimuscarinic Agents
- Gastric Emptying in Patients with Gastric Ulcers : Effects of Oral and Intramuscular Administration of Anticholinergic Drug
- Epidemio-hygieological Aspects of Gastric Neoplasms in Aged Patients
- Relation between Esophagogastric Junction and Esophageal Function in Patients with Reflux Esophagitis
- Gastric Acid Secretion and Plasma Gastrin Response to Test Meal in Patients with Gastric Cancer
- Esophageal Ulceration in a Patient with Behcet's Syndrome
- A Comparative Study on Application of Nd-YAG Laser Radiation and Electrocautery in Canine Gastric Mucosa : Magnified Observations by Dissecting Microscopy and Scanning Electron Microscopy, and Histological Examinations
- Clinical Investigations in Patients with Juvenile Gastric Atrophy Compared with Senile Gastric Atrophy
- The PFD Test for Pancreatic Disease Patients
- The Coexistence of Gastric Cancer and Duodenal Ulcers
- Hematemesis and Melena : Gastritis
- The Effects of Test Meals on the PFD Test
- Reactive Hypoglycemia Associated with Transient Hyperthyroidism in the Background of the Prediabetic Stage
- Esophageal Ulceration in a Patient with Behcet's Syndrome
- Hematemesis and Melena : Gastric Ulcer
- Hematemesis and Melena : Mallory-Weiss Syndrome
- Hematemesis and Melena : Endoscopic Examination
- Esophageal Ulceration in a Patient with Behcet's Syndrome
- Conditioning of Karyoplasts for Producing Somatic Nuclear Transferred Gonadal Germ Cells in Domestic Chickens
- Production of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian Species
- Cryopreservation of Gonadal Germ Cells (GGCs) from the Domestic Chicken Using Vitrification
- The Migratory Ability of Gonadal Germ Cells in the Domestic Chicken
- Attempt to produce nuclear transferred primordial germ cells using electrofusion in domestic chicken
- Simplifield DNA Extraction Methods for Sexing Chick Embryos
- Effects of Ultraviolet Irradiation on the Migratory Ability of Primordial Germ Cells (PGCs) in the Domestic Chicken
- セレニウム・ビタミンE注射は暑熱負荷時の山羊の好中球ニトロブルーテトラゾリウム還元能に影響を及ぼさない
- 水牛における季節的環境変化に対する生理反応および血液成分の牛と比較した特徴
- 沖縄島漫湖饒波川のマングローブ域における大型底生生物の分布と出現量
- Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
- Comparison of carcass and meat characteristics of Brahman grade cattle (Bos indicus) and crossbred water buffalo (Bubalus bubalis) fed on high roughage diet
- Comparison of fattening performance in Brahman grade cattle (Bos indicus) and crossbred water buffalo (Bubalus bubalis) fed on high roughage diet
- Comparison of carcass and meat characteristics of Brahman grade cattle (Bos indicus) and crossbred water buffalo (Bubalus bubalis)
- Comparison of feed intake, digestibility and fattening performance of Brahman grade cattle (Bos indicus) and crossbred water buffalo (Bubalus bubalis)
- Effect of Cryoprotectant Composition on In Vitro Viability of In Vitro Fertilized and Cloned Bovine Embryos Following Vitrification and In-Straw Dilution
- 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
- Vitrification of Fully Grown and Growing Porcine Oocytes Using Germinal Vesicle Transfer
- Deterioration of Retinopathy After Starting Interferon Therapy for Chronic Hepatitis C with Diabetic Triopathy
- Secondary Hemochromatosis Accompanied by Diabetes Mellitus and Hypogonadism.
- Seroconversion of Glutamic Acid Decarboxylase Antibodies in a Patient Initially Diagnosed as Having Type 2 Diabetes Mellitus
- Metformin Use in an Obese Diabetic Patient from Weeks 1 to 21 of Pregnancy.
- Sarcoid Granulomatous Interstitial Nephritis Accompanied by Type 2 Diabetes Mellitus
- Graves' Disease Accompanied by Anti-myeloperoxidase Antibody-related Nephropathy and Autoimmune Hepatitis
- 17α-Hydroxylase Deficiency Accompanied by Adrenal Myelolipoma
- マレイシアにおける沼沢水牛(Bubalus bubalis)およびKedah-Kelantan牛(Bos indicus)から回収した卵胞卵子の体外受精
- ヤギにおける採食量に及ぼす第三脳室内 Cholecystokinin 投与の影響
- Developmental Competence of Cat Oocytes from Ovaries Stored at Various Temperature for 24 h
- シクロヘキシミドへの曝露が電気的に活性化したネコ卵母細胞の発育に及ぼす効果
- Effects of Heat Stress on Follicular Development in PMSG-treated Immature Rats
- Effects of Heat Stress on Follicular Development in PMSG-treated Immature Rats
- Taxonomy of the Terrestrial Crab Geothelphusa tenuimanus (Crustacea: Decapoda: Brachyura: Potamidae) of Okinawa Island, Central Ryukyus, Japan
- 培養卵丘細胞をドナー核とした体外成熟ブタ卵母細胞の核移植後の発生能力
- 暑熱ストレスがマウス卵管酸化還元状態および初期胚発生に及ぼす影響
- Evaluation of Developmental Competence of In Vitro-produced Porcine Embryos Based on the Timing, Pattern and Evenness of the First Cleavage and Onset of the Second Cleavage
- Intracytoplasmic Sperm Injection in the Pig : Where is the Problem?
- Nuclear and Cytoplasmic Maturation In Vitro of Porcine Oocytes
- Comparison of voluntary feed intake, rumen passage and degradation kinetics between crossbred Brahmam cattle (Bos indicus) and swamp buffaloes (Bubalus bubalis) fed a fattening diet based on corn silage
- Effect of Bezafibrate or Pravastain on Serum Lipid Levels and Albuminuria in MIDDM Patients
- Effect of Nephropathy on the Composition of Apolipoprotein-Containing Particles in NIDDM
- Prader-Willi Syndrome with Elevated Follicle Stimulating Hormone Levels and Diabetes Mellitus
- Diabetes Mellitus Accompanied by Nonocclusive Colonic Ischemia
- Candida Glabrata Fungemia After Amputation of an Infected Diabetic Foot
- Cervical Spinal Epidural Abscess and Perirenal Abscess Associated with Diabetes Mellitus
- An approach for conservation of pig resources by xenograft of gonadal tissue to mice
- Effect of Centrifugation Treatment before Vitrification on the Viability of Porcine Mature Oocytes and Zygotes Produced In Vitro
- Fine Mapping of a Region of Rat Chromosome 12 Close to the Aspermia (as) Locus and Comparison with the Human Orthologous Regions
- Endocrine Status and Development of Porcine Testicular Tissues in Host Mice
- Affected Homologous Chromosome Pairing and Phosphorylation of Testis Specific Histone, H2AX, in Male Meiosis Under FKBP6 Deficiency
- Changes in Circulating and Testicular Levels of Inhibin A and B During Postnatal Development in Bulls
- Development In Vivo and In Vitro to Blastocysts of Porcine Oocytes Matured and Fertilized In Vitro
- 暑熱環境下における沼沢水牛の血液量変動による直腸-皮膚温度差の制御 : スイギュウとウシの体温調節に関する比較研究
- 暑熱環境下における沼沢水牛の血液量変動による直腸-皮膚温度差の制御 : スイギュウとウシの体温調節に関する比較研究
- A Mixed - Valence Mn(II,III) Complex with a 2D Structure:Crystal Structure and Magnetic Property of K[Mn_2(u - CrO_4)_2(u - OH)_2]
- Isolation and Identification of F-Spondin in the Boar Testis and Its Production During Testis Growth
- 形質転換Cos細胞およびニワトリ脳に発現したニワトリKitチロシンリン酸化酵素の生化学的性状
- Viability of maternally heat-stressed mouse zygotes in vivo and in vitro
- 肉用種ドナー群の卵胞嚢腫発生に対する膣内留置型プロジェステロン製剤の有効性
- 体外受精で得られた水牛初期胚における染色体異常の出現率
- Evaluation of Developmental Competence of In Vitro-produced Porcine Embryos Based on the Timing, Pattern and Evenness of the First Cleavage and Onset of the Second Cleavage
- 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
- Eating and rumination behaviour in Brahman grade cattle and crossbred water buffalo fed on high roughage diet
- Effects of Heat Stress on the Redox Status in the Oviduct and Early Embryonic Development in Mice
- Developmental competence and glutathione content of maternally heat-stressed mouse oocytes and zygotes
- Electrophysiological Recording of Hypothalamic GnRH Pulse Generator Activity in Castrated Male Goats
- Identification of tamaraw (Bubalus mindorensis) from natural habitat-derived fecal samples by PCR-RFLP analysis of cytochrome b gene
- Expression Analysis of MIF4GD in the Rat Testis
- Factors Affecting Fertilization and Embryonic Development During Intracytoplasmic Sperm Injection in Pigs
- スイギュウの繁殖 (特集 動物・家畜の繁殖)
- Vitrification of Fully Grown and Growing Porcine Oocytes Using Germinal Vesicle Transfer
- Hypogonadism in Heat Stressed Goats : Poor Responsiveness of the Ovary to the Pulsatile LH Stimulation Induced by Hourly Injections of a Small Dose of GnRH
- Increased mass of slow-type skeletal muscles and depressed myostatin gene expression in cold-tolerant chicks
- Improvement of Porcine Oocytes with Low Developmental Ability after Fusion of Cytoplasmic Fragments Prepared by Serial Centrifugation
- In vitro production of porcine embryos : current status, future perspectives and alternative applications
- Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm
- Xenografting of gonadal tissues into mice as a possible method for conservation and utilization of porcine genetic resources
- Factors Affecting Cryopreservation of Porcine Oocytes
- Role of prolactin-like protein (PRL-L) in cold-induced increase of muscle mass in chicks
- I はじめに