Endocrine Status and Development of Porcine Testicular Tissues in Host Mice

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概要

• 論文の詳細を見る

• Clarification of the endocrine status of host mice provides us with basic knowledge with which we can manipulate the growth and function of xenografted testicular tissues. We investigated the hormonal profiles of castrated mice grafted with porcine immature testicular tissues from 30 to 210 or more days after grafting (day 0=castration and grafting). The serum FSH concentrations of the host mice declined (P<0.05) from day 60 compared with those of the castrated, ungrafted mice. The serum inhibin and testosterone levels were higher (P<0.05) than those in the castrated, ungrafted mice from days 30 and 90 days, respectively. The inhibin levels further increased (P<0.05) from day 90, during which time the levels were higher (P<0.05) than those in the intact male mice. In the grafts, formation of lumens occurred in the seminiferous cords on day 90 and spermatozoa appeared in the lumens from day 120. However, spermatogenesis in the grafts did not reach the qualitatively normal levels observed in adult boars. The intensity of the immune reaction to inhibin α subunits in the Sertoli cells of the grafts decreased with differentiation of the seminiferous tubules. The present findings indicate that a feedback loop was established between the mouse hypothalamo-pituitary axis and the grafted porcine tissues from 60 days post-grafting. The results also indicate that the serum inhibin levels in the host mice remained high even after the appearance of lumens in the seminiferous tubules of the grafted tissues; this is strikingly different to the situation in normal male animals, in which the serum inhibin levels decline at around the time of tubular differentiation. The lack of efferent ducts in the tubules of the grafted tissues probably caused the accumulation of inhibin to be released into the lumens, resulting in high concentrations of circulating inhibin. These high levels of inhibin may directly affect spermatogenic activity and suppress FSH secretion.

• 日本繁殖生物学会の論文
• 2008-12-01

著者

• Kikuchi Kazuhiro

  東京農工大学 大学院農学府

• Kikuchi Kazuhiro

  Dep. Of Animal Sci. National Inst. Of Agrobiological Sciences

• Kikuchi Kazuhiro

  Department Of Animal Science National Institute Of Agrobiological Sciences

• NAKAI Michiko

  Division of Animal Sciences, National Institute of Agrobiological Sciences

• KANEKO Hiroyuki

  Division of Animal Sciences, National Institute of Agrobiological Sciences

• Kaneko Hiroyuki

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• KANEKO Hiroyuki

  Reproductive Biology Research Unit, National Institute of Agrobiological Sciences

• KIKUCHI Kazuhiro

  Reproductive Biology Research Unit, National Institute of Agrobiological Sciences

• NAKAI Michiko

  Reproductive Biology Research Unit, National Institute of Agrobiological Sciences

• NOGUCHI Junko

  Reproductive Biology Research Unit, National Institute of Agrobiological Sciences

• Kaneko H

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• Noguchi J

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• Noguchi Junko

  Positron Medical Center Tokyo Metropolitan Institute Of Gerontology

• Noguchi Junko

  Reproductive Biology Research Unit Division Of Animal Sciences National Institute Of Agrobiological

• Nakai Michiko

  Division Of Animal Sciences National Institute Of Agrobiological Sciences

• NOGUCHI Junko

  Reproductive Biology Research Unit (Kannondai), Division of Animal Sciences, National Institute of Agrobiological Sciences

• KANEKO Hiroyuki

  Reproductive Biology Research Unit (Kannondai), National Institute of Agrobiological Science

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