Development In Vivo and In Vitro to Blastocysts of Porcine Oocytes Matured and Fertilized In Vitro
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概要
- 論文の詳細を見る
The objective of the present study was to evaluate the development to the blastocyst stage in vivo and in vitro of porcine oocytes matured and fertilized in vitro (IVM/IVF). When IVM/IVF oocytes had been transferred to oviducts of synchronized recipients just after IVF, more than 70% of blastocysts recovered after 6 days of transfer had developed to the hatched blastocyst stage with a mean cell number of 181.5 per blastocyst. In contrast, when IVM/IVF oocytes were cultured in vitro for 2 days and then transferred to the oviducts of recipients, or cultured in vitro for 6 days without transfer to oviducts, most recovered blastocysts were still enclosed by zona pellucidae. Mean cell numbers of blastocysts obtained from IVM/IVF oocytes cultured for 2 days in vitro plus 4 days in the oviducts and for 6 days in vitro decreased significantly (P<0.01) to 58.2 and 38.4, respectively, compared with that of blastocysts obtained from IVM/IVF oocytes cultured in oviducts for 6 days. These results suggest that porcine IVM/IVF oocytes have a high potential for developing to the blastocyst stage equal to that of in vivo matured oocytes and that the in vitro culture conditions used for IVM/IVF oocytes in the present study were suboptimal.
- 日本繁殖生物学会の論文
著者
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KASHIWAZAKI Naomi
Laboratory of Animal Reproduction, Graduate School of Veterinary Science, Azabu University
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Kikuchi Kazuhiro
東京農工大学 大学院農学府
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NAGAI Takashi
National Institute of Livestock and Grassland Science
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Kikuchi Kazuhiro
Dep. Of Animal Sci. National Inst. Of Agrobiological Sciences
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Kikuchi Kazuhiro
National Institute For Basic Biology
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Suzuki Kaoru
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
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NOGUCHI Junko
National Institute of Agrobiological Sciences
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KANEKO Hiroyuki
National Institute of Agrobiological Sciences
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SHINO Masao
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
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Kashiwazaki Naomi
Laboratory Of Animal Reproduction Azabu University
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Shino Masao
Laboratory Of Animal Reproduction Department Of Animal Science And Biotechnology School Of Veterinar
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Nagai Takashi
National Inst. Of Livestock And Grassland Sci.
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