Effect of Donor Cell Types on Developmental Potential of Cattle (Bos taurus) and Swamp Buffalo (Bubalus bubalis) Cloned Embryos

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概要

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• This study investigated the effect of donor cell types on the developmental potential and quality of cloned swamp buffalo embryos in comparison with cloned cattle embryos. Fetal fibroblasts (FFs), ear fibroblasts (EFs), granulosa cells (GCs) and cumulus cells (CCs) were used as the donor cells in both buffalo and cattle. The cloned cattle or buffalo embryos were produced by fusion of the individual donor cells with enucleated cattle or buffalo oocytes, respectively. The reconstructed (cloned) embryos and in vitro matured oocytes without enucleation were parthenogenetically activated (PA) and cultured for 7 days. Their developmental ability to the blastocyst stage was evaluated. The total number of trophectoderm (TE) and inner cell mass (ICM) cells and the ICM ratio in each blastocyst was determined by differential staining as an indicator of embryo quality. The fusion rate of CCs with enucleated oocytes was significantly lower than for those of other donor cell types both in cattle and buffalo. The rates of cleavage and development to the 8-cell, morula and blastocyst stages of cloned embryos derived from all donor cell types did not significantly differ within the same species. However, the cleavage rate of cloned cattle embryos derived from FFs was significantly higher than those of cattle PA and cloned buffalo embryos. The blastocyst rates of cloned cattle embryos, except for the ones derived from CCs, were significantly higher than those of cloned buffalo embryos. In buffalo, only cloned embryos derived from CCs showed a significantly higher blastocyst rate than that of PA embryos. In contrast, all the cloned cattle embryos showed significantly higher blastocyst rates than that of PA embryos. There was no difference in ICM ratio among any of the blastocysts derived from any of the donor cell types and PA embryos in both species. FFs, EFs, GCs and CCs had similar potentials to support development of cloned cattle and buffalo embryos to the blastocyst stage with the same quality.

著者

• IMSOONTHORNRUKSA Sumeth

  Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te

• LORTHONGPANICH Chanchao

  Embryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Te

• Phermthai Tatsanee

  Department Of Obstetrics And Gynecology Faculty Of Medicine Siriraj Hospital

• Laowtammathron Chuti

  Embryo Technology And Stem Cell Research Center And School Of Biotechnology Suranaree University Of

• Ketudat-cairns Mariena

  Embryo Technol. And Stem Cell Res. Center School Of Biotechnology Suranaree Univ. Of Technol.

• Parnpai Rangsun

  Embryo Technol. And Stem Cell Res. Center School Of Biotechnology Suranaree Univ. Of Technol.

• Nagai Takashi

  National Inst. Of Livestock And Grassland Sci.

• Srirattana Kanokwan

  Embryo Technol. And Stem Cell Res. Center School Of Biotechnology Suranaree Univ. Of Technol.

• Laowtammathron Chuti

  Embryo Technol. And Stem Cell Res. Center School Of Biotechnology Suranaree Univ. Of Technol.

• Imsoonthornruksa Sumeth

  Embryo Technology And Stem Cell Research Center School Of Biotechnology Suranaree University Of Tech

• Lorthongpanich Chanchao

  Embryo Technology And Stem Cell Research Center School Of Biotechnology Suranaree University Of Tech

• SRIRATTANA Kanokwan

  Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology

• IMSOONTHORNRUKSA Sumeth

  Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology

• PARNPAI Rangsun

  Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology

• KETUDAT-CAIRNS Mariena

  Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology

• LAOWTAMMATHRON Chuti

  Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology

• LORTHONGPANICH Chanchao

  Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology

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