Effects of Cysteine During In Vitro Maturation of Porcine Oocytes Under Low Oxygen Tension on Their Subsequent In Vitro Fertilization and Development
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概要
- 論文の詳細を見る
In this study, we evaluated the effect of different concentrations of cysteine in in vitro maturation (IVM) medium during IVM under low oxygen tension (5% O2) of porcine oocytes on the intercellular content of glutathione (GSH) and subsequent in vitro fertilization (IVF) and development. Cumulus oocyte complexes (COCs) were collected from ovaries obtained at a local slaughterhouse, cultured in IVM medium supplemented with 0 (control), 0.05, 0.1, 0.2 or 0.6 mM cysteine for 44-46 h, fertilized in vitro and subsequently cultured for 6 days in total. The GSH content of the IVM oocytes exposed to 0, 0.05, 0.1, 0.2 or 0.6 mM cysteine increased significantly (P<0.05) as the concentration of cysteine increased (12.2, 14.0, 15.1, 16.4 and 16.4 pmol/oocyte, respectively). However, the rates of oocyte maturation, sperm penetration, male pronuclear formation, monospermy and even cleavage on Day 2 (the day of IVF was defined as Day 0) and blastocyst formation on Day 6 did not differ among the groups. Moreover, the cell numbers of blastomeres in blastocysts were uniform among the groups. These results indicate that supplementation with 0.05-0.6 mM cysteine during IVM under 5% O2 tension significantly increased the intracellular GSH contents of IVM oocytes; however, it had no promoting effects on nuclear maturation, fertilization, male pronucleus formation and subsequent embryonic development to the blastocyst stage.
- 日本繁殖生物学会の論文
- 2009-12-01
著者
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NAGAI Takashi
National Institute of Livestock and Grassland Science
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Dang-nguyen Thanh
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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NGUYEN Bui
Institute of Biotechnology, Vietnamese Academy of Science and Technology
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MANABE Noboru
Graduate School of Agricultural and Life Sciences, University of Tokyo
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永井 卓
(独)・農業・生物系特定産業技術研究機構・畜産草地研究所
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Nguyen Bui
Institute Of Biotechnology Vietnamese Academy Of Science And Technology
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Manabe Noboru
Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo
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Manabe Noboru
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Manabe Noboru
Animal Resource Science Center Graduated School Of Agriculture And Life Science University Of Tokyo
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VIET LINH
National Institute of Livestock and Grassland Science
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DANG-NGUYEN Thanh
National Institute of Livestock and Grassland Science
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Manabe Noboru
Animal Resource Science Center The University Of Tokyo
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Manabe Noboru
Animal Resource Sci. Center Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Nagai T
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Nagai Takuya
Nasu Institute Nisshin Flour Milling Co. Ltd.
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Viet-linh Nguyen
Department Of Animal Science National Institute Of Agrobiological Sciences
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Nagai Takashi
National Inst. Of Livestock And Grassland Sci.
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Manabe Noboru
Animal Resource Sci. Center Veterinary Medical Sci. The Univ. Of Tokyo
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