Expression and Function of Apoptosis Initiator FOXO3 in Granulosa Cells During Follicular Atresia in Pig Ovaries
スポンサーリンク
概要
- 論文の詳細を見る
In mammalian ovaries, most follicles are lost by atresia before ovulation. It has become apparent that the apoptosis of granulosa cells induces follicular atresia. Forkhead box O3 (FOXO3), also called FKHRL1 (forkhead in rhabdomyosarcoma-like 1), is a proapoptotic molecule that belongs to the FOXO subfamily of forkhead transcription factors. Foxo3-deficient female mice were reported to be infertile because of abnormal ovarian follicular development, but the precise influences of FOXO3 on follicular atresia of mature ovary have not been determined. Therefore, we examined the expression and function of FOXO3 in porcine ovarian follicles and granulosa-derived cells. FOXO3 mRNA levels in granulosa cells of porcine ovaries increased during atresia, while FOXO3 protein was abundant in granulosa cells of early atretic follicles. By immunohistochemistry, the inner surface area of the granulosa layer in early atretic follicles was strongly stained with anti-FOXO3 antibody. The granulosa cells expressing FOXO3 coincided with apoptotic cells, indicating a role of FOXO3 as a proapoptotic factor in granulosa cells of porcine ovaries. In porcine (JC-410) and human (KGN) granulosa-derived cells, cell death was induced by transfection of FOXO3 expression vectors. Expression of the proapoptotic factors Fas ligand (FASLG) and BCL2-like 11 (BCL2L11) was upregulated by FOXO3 in KGN cells. In conclusion, FOXO3 is expressed in porcine ovarian follicles and induces apoptosis in granulosa cells, suggesting that it is a candidate for the initiator of follicular atresia.
- 2011-02-01
著者
-
MANABE Noboru
Research Unit of Animal Life Sciences, Animal Resource Science Centre, The University of Tokyo
-
Inoue Naoko
Laboratory Of Animal Morphology And Function Graduate School Of Bioagricultural Sciences Nagoya Univ
-
MAEDA Akihisa
Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
-
SAKAMAKI Kazuhiro
Laboratory of Tumore Cell Biology, Department of Life Sciences, Kyoto University
-
Manabe Noboru
Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo
-
Manabe Noboru
Graduate School Of Agricultural And Life Sciences University Of Tokyo
-
Manabe Noboru
Animal Resource Science Center Graduated School Of Agriculture And Life Science University Of Tokyo
-
Manabe Noboru
Animal Resource Science Center The University Of Tokyo
-
MAEDA Akihisa
Animal Resource Science Center, The University of Tokyo
-
GOTO Yasufumi
Animal Resource Science Center, The University of Tokyo
-
MATSUDA Fuko
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
-
GOTO Yasufumi
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
-
CHENG Yuan
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
-
Sakamaki Kazuhiro
Laboratory Of Tumor Cell Biology Department Of Life Sciences Kyoto University
-
Sakamaki Kazuhiro
Graduate School Of Biostudies And Institute For Virus Research Kyoto University
-
Sakamaki Kazuhiro
Laboratory Of Developmental Cell Biology Division Of Systemic Life Science Graduate School Of Biostu
-
Cheng Yuan
Animal Resource Science Center The University Of Tokyo
-
Sai Takafumi
Animal Resource Science Center Graduate School Of Agricultural And Life Sciences University Of Tokyo
-
Matsuda Fuko
東京大学大学院農学生命科学研究科高等動物教育研究センター附属牧場
-
Gonda Hiroshi
Animal Resource Science Center The University Of Tokyo
-
SAI Takafumi
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
-
GONDA Hiroshi
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
-
Manabe Noboru
Animal Resource Sci. Center Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
-
Matsuda F
Univ. Tokyo Tokyo Jpn
-
Inoue Naoko
Unit Of Anatomy And Cell Biology Department Of Animal Sciences Kyoto University
-
Manabe Noboru
Research Center For Food Safety Graduate School Of Agricultural And Life Sciences The University Of
-
Maeda Akihisa
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
-
Goto Yasufumi
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
-
Matsuda-minehata Fuko
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
-
Cheng Yuan
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
-
Sai Takafumi
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
-
Gonda Hiroshi
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
-
Inoue Naoko
Laboratory Of Animal Morphology And Function Division Of Biofunctional Development Graduate School Of Bioagricultural Sciences Nagoya University
関連論文
- Metabolic Conversion of Zearalenone to α-Zearalenol by Goat Tissues
- 異種移植したブタ卵巣組織における卵胞と卵母細胞の発育と成熟およびその体外成熟培養
- Profiles of Circulating Steroid Hormones, Gonadotropins, Immunoreactive Inhibin and Prolactin During Pregnancy in Goats and Immunolocalization of Inhibin Subunits, Steroidogenic Enzymes and Prolactin in the Corpus Luteum and Placenta
- Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
- 経胎盤ビスフェノールA(BPA)暴露が器官形成期のマウス胎仔におけるarylhydrocarbon受容体(AhR)とレチノイン酸受容体mRNA発現におよぼす影響
- 経胎盤的ビスフェノールA暴露がマウス胎仔におけるアリルヒドロカーボン受容体とその関連因子および生体異物代謝酵素の発現におよぼす影響
- ブタ卵胞における選択的閉鎖の制御機構:顆粒層細胞アポトーシスの制御
- ブタ卵胞顆粒層細胞アポトーシスにおけるTRAILシグナル経路の役割
- ブタ卵巣の卵胞閉鎖における顆粒層細胞アポトーシス誘導にTRADDが必要である
- ブタ卵巣の閉鎖卵胞顆粒層細胞ではTRAIL囮受容体-1が消失する
- ブタ卵胞顆粒層細胞膜上の抗原を認識するモノクローナル抗体
- 造血細胞移植後のホーミングにおけるMAdCAM-1およびそのリガンドの役割
- ブタ卵胞閉鎖にともなうコネキシン43の発現と局在の推移
- ブタcellular FLICE-like inhibitory protein遺伝子のクローニングと発現解析
- Expression and Localization of cFLIP, an Anti-apoptotic Factor, in the Bovine Corpus Luteum
- Molecular Cloning of a Porcine (Sus scrofa) Apoptosis Inhibitory Ligand, Netrin-1, and Its Receptor, p53RDL1
- ICGNマウスにおける組織トランスグルタミナーゼ(tTG)の局在
- Regulation of Interferon-τ Gene Expression and the Maternal Recognition of Pregnancy
- Changes in the Expression of Decoy Receptor 3 in Granulosa Cells During Follicular Atresia in Porcine Ovaries
- Expression and Function of Apoptosis Initiator FOXO3 in Granulosa Cells During Follicular Atresia in Pig Ovaries
- Apoptosis Occurs in Granulosa Cells but not Cumulus Cells in the Atretic Graafian Follicles in Multiparous Pig Ovaries
- 様々なフタレート類におけるin vitroとin vivoの評価モデル間でみられるエストロゲン様活性の矛盾はCalbindin-D9kの発現と関連した現象として理解できる
- Effects of Cysteine During In Vitro Maturation of Porcine Oocytes Under Low Oxygen Tension on Their Subsequent In Vitro Fertilization and Development
- Changes in Expression and Localization of GPRC5B and RARα in the Placenta and Yolk Sac During Middle to Late Gestation in Mice
- ヤギ下垂体における塩基性線維芽細胞成長因子の局在に関する免疫組織化学的研究
- Changes in Expression and Localization of X-linked Inhibitor of Apoptosis Protein (XIAP) in Follicular Granulosa Cells During Atresia in Porcine Ovaries
- cFLIP Regulates Death Receptor mediated Apoptosis in an Ovarian Granulosa Cell Line by Inhibiting Procaspase-8 Cleavage
- Regulation of granulosa cell apoptosis by death ligand-receptor signaling
- Molecular Cloning of Porcine (Sus scrofa) Tumor Necrosis Factor Receptor 2
- Changes in Expression of Interleukin-6 Receptors in Granulosa Cells During Follicular Atresia in Pig Ovaries
- The Regulation of Ovarian Granulosa Cell Death by Pro- and Anti-apoptotic Molecules
- Expression and Localization of Fas Ligand and Fas During Atresia in Porcine Ovarian Follicles
- Granulosa cell is type II apoptosis-cell and regulates follicular atresia in mammalian ovaries(Development, Reproduction, Proceedings of the 32nd Annual Meeting)
- Physiological Roles of Inhibin on Follicular Development during Early Pregnancy in Shiba Goats
- Expression and function of apoptosis initiator FOXO3 in granulosa cells during follicular atresia in pig ovaries
- The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis during Follicular Atresia in Pig Ovaries
- Molecular Characteristics of Porcine Fas-associated Death Domain (FADD) and Procaspase-8
- ハンガリー在来ブタ(Mangalica)とLandrace雌ブタの排卵期における卵胞・卵母細胞の発育・成熟と生殖ホルモン分泌の比較
- Bid and Bax Are Involved in Granulosa Cell Apoptosis During Follicular Atresia in Porcine Ovaries
- ブタ退行卵胞顆粒層細胞における細胞外マトリックスとアポトーシスの相互作用
- Effect of the Mycelial Extract of Cultured Cordyceps Sinensis on In Vivo Hepatic Energy Metabolism in the Mouse
- Role of Cell-Death Ligand-Receptor System of Granulosa Cells in Selective Follicular Atresia in Porcine Ovary
- Activity Enhancement by Introduction of Two Different Halogen Atoms into Phe-2-phenyl Group of Thrombin Receptor Tethered-Ligand Analogs
- The Characteristics of Anemia in Hereditary Nephrotic (ICGN) Mice
- ブタ卵胞顆粒層細胞の新規なアポトーシス受容体と囮受容体を認識するモノクローナル抗体
- Immunohistochemical Quantification of Fast-Myosin in Upper Hind Limb Muscles of the Pig
- Conflict of Estrogenic Activity by Various Phthalates between In Vitro and In Vivo Models Related to the Expression of Calbindin-D_
- Differential Transcriptional and Translational Regulations of Calbindin-D_ by Steroid Hormones and Their Receptors in the Uterus of Immature Mice
- Ca^/Mg^-Dependent Endonuclease but not Ca^-Dependent, Mg^-Dependent or Cation-Independent Endonuclease is Involved in Granulosa Cell Apoptosis of Pig Atretic Follicles
- Molecular Characteristics of Porcine Fas-associated Death Domain (FADD) and Procaspase-8
- Growth and Maturation of Follicles and Oocytes Following Xenotransplantation of Porcine Ovarian Tissues and In Vitro Maturation
- Changes in Expression and Localization of Connexin 43 mRNA and Protein in Porcine Ovary Granulosa Cells during Follicular Atresia
- Effects of Exposure In Utero to Bisphenol A on the Expression of Aryl Hydrocarbon Receptor, Related Factors, and Xenobiotic Metabolizing Enzymes in Murine Embryos
- Effects of In Utero Exposure to Bisphenol A on mRNA Expression of Arylhydrocarbon and Retinoid Receptors in Murine Embryos
- Porcine (Sus scrofa) Cellular FLICE-like Inhibitory Protein (cFLIP) : Molecular Cloning and Comparison with the Human and Murine cFLIP
- Regulation Mechanism of Selective Atresia in Porcine Follicles : Regulation of Granulosa Cell Apoptosis during Atresia
- Comparison of Follicular and Oocyte Development and Reproductive Hormone Secretion during the Ovulatory Period in Hungarian Native Breed, Mangalica, and Landrace Gilts
- Changes in Cell Adhesion Molecules during Follicular Atresia in Porcine Ovaries
- ウシの卵胞におけるinhibin α-subunitのステージ特異的な分布と卵胞液中の各種ホルモンレベルについて
- Ovarian Follicular Dynamics and Hormonal Changes in Goats during Early Pregnancy
- Age-related thymic involution is mediated by Fas on thymic epithelial cells
- Reduction of Thymocyte Numbers in Transgenic Mice Expressing Viral FLICE-Inhibitory Protein in a Fas-Independent Manner
- Ultrastructural Changes in Granulosa Cells in Porcine Antral Follicles Undergoing Atresia Indicate Apoptotic Cell Death
- Alterations in Cell Proliferation and Morphology of Ampullar Epithelium of the Mouse Oviduct during the Estrous Cycle
- Expression and Function of Apoptosis Initiator FOXO3 in Granulosa Cells During Follicular Atresia in Pig Ovaries
- Embryo Implantation is Blocked by Intraperitoneal Injection with Anti-LIF Antibody in Mice
- ブタおよびウシ卵胞退行中の顆粒層および内卵胞膜細胞におけるアポトーシス細胞局在の種差について
- Incorporation of β-amyloid protein through the bovine ileal epithelium before and after weaning : Model for orally transmitted amyloidoses
- Examination of Granulosa Cell Glycoconjugates Which Change During Follicular Atresia in the Pig Ovary
- Role of Cell-Death Ligand-Receptor System of Granulosa Cells in Selective Follicular Atresia in Porcine Ovary
- Vitrification of Fully Grown and Growing Porcine Oocytes Using Germinal Vesicle Transfer
- Bid and Bax Are Involved in Granulosa Cell Apoptosis During Follicular Atresia in Porcine Ovaries
- Effect of RNA Interference of BID and BAX mRNAs on Apoptosis in Granulosa Cell-derived KGN Cells
- Physiological and Pathological Cell Deaths in the Reproductive Organs
- Improvement of Porcine Oocytes with Low Developmental Ability after Fusion of Cytoplasmic Fragments Prepared by Serial Centrifugation
- Follicular Growth and Atresia in Mammalian Ovaries: Regulation by Survival and Death of Granulosa Cells
- Effect of RNA Interference of BID and BAX mRNAs on Apoptosis in Granulosa Cell-derived KGN Cells
- Embryo Implantation is Blocked by Intraperitoneal Injection with Anti-LIF Antibody in Mice
- Follicular Growth and Atresia in Mammalian Ovaries : Regulation by Survival and Death of Granulosa Cells
- Follicular Turnover and Hormonal Association in Postpartum Goats During Early and Late Lactation
- Effects of Long-Term Grafting on Follicular Growth in Porcine Ovarian Cortical Grafts Xenoplanted to Severe Combined Immunodeficient (SCID) Mice
- Experimental Evidence for the Influence of Cumulus-Oocyte-Complexes on Sperm Release from the Porcine Oviductal Sperm Reservoir