Effect of RNA Interference of BID and BAX mRNAs on Apoptosis in Granulosa Cell-derived KGN Cells
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概要
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In mitochondrion-dependent type II apoptosis, BH3-interacting domain death agonist (BID) and BCL-2-associated X protein (BAX) promote death ligand and receptor-mediated cell death. In porcine ovaries, the levels of BID and BAX increase in follicular granulosa cells during atresia. In the present study, to confirm the pro-apoptotic activity of BID and BAX in granulosa cells, we examined the effect of RNA interference of BID or BAX on apoptosis using a human ovarian granulosa tumor cell line, KGN. By reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, expression of BID and BAX was detected in KGN cells. Then, we suppressed BID and BAX mRNA expression in KGN cells using small interfering RNA (siRNA). When BID or BAX was suppressed, a significant decrease in the apoptotic cell rate was noted. In granulosa-derived cells, BID and BAX showed pro-apoptotic activity. These results suggest that BID and BAX act as signal-transducing factors in mitochondrion-dependent type II apoptosis.
著者
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Manabe Noboru
Animal Resource Center The University Of Tokyo:research Center. For Food Safety The University Of To
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MATSUDA Fuko
Animal Resource Science Center, The University of Tokyo
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Sugimoto Miki
Graduate School Of Agriculture Kyoto Univ.
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Sai Takafumi
Animal Resource Science Center Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Matsuda Fuko
東京大学大学院農学生命科学研究科高等動物教育研究センター附属牧場
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LI Jun-You
Animal Resource Science Center, The University of Tokyo
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Matsuda-minehata Fuko
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Kabir Abul
Animal Resource Science Center The University Of Tokyo
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Maeda Akihisa
Animal Resource Science Center The University Of Tokyo
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Gao Hong-mei
Animal Resource Science Center The University Of Tokyo
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Goto Yasufumi
Animal Resource Science Center The University Of Tokyo
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MANABE Noboru
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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MATSUDA Fuko
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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KABIR Abul
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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LI Jun-You
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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GAO Hong-Mei
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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SUGIMOTO Miki
Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
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SAI Takafumi
Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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