Changes in Expression of Interleukin-6 Receptors in Granulosa Cells During Follicular Atresia in Pig Ovaries
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概要
- 論文の詳細を見る
More than 99% of follicles undergo a degenerative process known as atresia in mammalian ovaries, and only a few follicles ovulate during follicular growth and development. Follicular selection predominantly depends on granulosa cell apoptosis. To reveal the molecular mechanisms of selective follicular atresia, we examined the changes in the levels of interleukin-6 (IL-6) receptors expressed in the granulosa cells of pig ovaries. The levels of IL-6 receptor (IL-6R) α mRNA and protein in granulosa cells were quantified by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. IL-6Rα mRNA and protein were highly expressed in the granulosa cells of progressed atretic follicles. Enzyme-linked immunosorbent assay (ELISA) showed that the expression of IL-6 soluble receptor (IL-6sR) protein in follicular fluid decreased during atresia. Moreover, we isolated porcine cDNA encoding an IL-6 signal transducer, gp130. Porcine gp130 (2754 bp and 917-aa) was identified from a cDNA library prepared using follicular granulosa cells of pig ovaries. Porcine gp130 was highly homologous with human and murine gp130. RT-PCR analysis revealed that the level of gp130 mRNA also decreased during atresia. We presume that IL-6sR and gp130, but not IL-6Rα, play important roles in regulation of granulosa cell survival.
- 日本繁殖生物学会の論文
- 2007-08-01
著者
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MANABE Noboru
Research Unit of Animal Life Sciences, Animal Resource Science Centre, The University of Tokyo
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Inoue Naoko
Laboratory Of Animal Morphology And Function Graduate School Of Bioagricultural Sciences Nagoya Univ
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MAEDA Akihisa
Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
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Manabe Noboru
Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo
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Manabe Noboru
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Manabe Noboru
Animal Resource Science Center Graduated School Of Agriculture And Life Science University Of Tokyo
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Manabe Noboru
Animal Resource Science Center The University Of Tokyo
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MAEDA Akihisa
Animal Resource Science Center, The University of Tokyo
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GOTO Yasufumi
Animal Resource Science Center, The University of Tokyo
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GOTO Yasufumi
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
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CHENG Yuan
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
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MATSUDA MINEHATA
Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
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Cheng Yuan
Animal Resource Science Center The University Of Tokyo
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Matsuda Fuko
東京大学大学院農学生命科学研究科高等動物教育研究センター附属牧場
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Manabe Noboru
Animal Resource Sci. Center Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Matsuda F
Univ. Tokyo Tokyo Jpn
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Inoue Naoko
Unit Of Anatomy And Cell Biology Department Of Animal Sciences Kyoto University
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Manabe Noboru
Research Center For Food Safety Graduate School Of Agricultural And Life Sciences The University Of
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Maeda Akihisa
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Goto Yasufumi
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Matsuda-minehata Fuko
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Cheng Yuan
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Inoue Naoko
Laboratory Of Animal Morphology And Function Division Of Biofunctional Development Graduate School Of Bioagricultural Sciences Nagoya University
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