Molecular Characteristics of Porcine Fas-associated Death Domain (FADD) and Procaspase-8
スポンサーリンク
概要
- 論文の詳細を見る
To reveal the intracellular signal transduction molecules involved in granulosa cell apoptosis in porcine ovarian follicles, we cloned the porcine Fas-associated death domain (FADD), an adaptor protein for the cell death receptor, and procaspase-8, an initiator caspase. Porcine FADD (pFADD) was 636 bp (211 amino acids: aa) long and showed 74.0 and 65.4% homology with human and murine FADD, respectively. Porcine procaspase-8 (pprocaspase-8) was 1,431 bp (476 aa) long and 70.6 and 63.4% homologous with human and murine procaspase-8, respectively. To confirm the apoptosis-inducing abilities, we constructed pFADD and pprocaspase-8 cDNA expression vectors with enhanced green fluorescence protein (EGFP) and then transfected them into human uterine cervix tumor (HeLa-K), human granulosa cell-derived (KGN), murine granulosa-derived tumor (KK1), and porcine granulosa-derived (JC410) cells. When pFADD and pprocaspase-8 were overexpressed, cell death was induced in these transfected cells. However when caspase-inhibitor p35 was cotransfected, cell death was inhibited. The pFADD and pprocaspase-8 genes are well conserved, as are the physiological functions of their products.
- 日本繁殖生物学会の論文
著者
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MANABE Noboru
Research Unit of Animal Life Sciences, Animal Resource Science Centre, The University of Tokyo
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Inoue Naoko
Laboratory Of Animal Morphology And Function Graduate School Of Bioagricultural Sciences Nagoya Univ
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SAKAMAKI Kazuhiro
Department of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University
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GOTO Yasufumi
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
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Matsuda-Minehata Fuko
Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
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Sakamaki Kazuhiro
Department Of Animal Development And Physiology Graduate School Of Biostudies Kyoto University
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Matsuda Fuko
東京大学大学院農学生命科学研究科高等動物教育研究センター附属牧場
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Inoue Naoko
Unit Of Anatomy And Cell Biology Department Of Animal Sciences Kyoto University
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Manabe Noboru
Research Center For Food Safety Graduate School Of Agricultural And Life Sciences The University Of
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Goto Yasufumi
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Matsuda-minehata Fuko
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Inoue Naoko
Laboratory Of Animal Morphology And Function Division Of Biofunctional Development Graduate School Of Bioagricultural Sciences Nagoya University
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