The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis during Follicular Atresia in Pig Ovaries
スポンサーリンク
概要
- 論文の詳細を見る
More than 99% of follicles in mammalian ovaries undergo a degenerative process known as atresia, and only a few follicles actually ovulate during follicular growth and development. Follicular selection mostly depends on granulosa cell apoptosis, but the molecular mechanism behind the regulation of this selective atresia is still largely unknown. In the present study, to examine whether or not interleukin-6 (IL-6), a multifunctional cytokine, is involved in apoptosis during atresia in pig ovaries, the expression of IL-6 mRNA in granulosa cells was quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR). The level of mRNA decreased during atresia. Enzyme-linked immunosorbent assay (ELISA) showed that the level of IL-6 protein in follicular fluid also decreased during atresia. Moreover, recombinant human IL-6 upregulated the expression of an intracellular apoptosis inhibitor, cellular FLICE-like inhibitory protein long form (cFLIPL), in cultured cells derived from human granulosa cells. It is possible that IL-6 is produced in the granulosa cells of healthy follicles, that it increases the cFLIPL level, and cFLIPL then prevents apoptotic cell death.
- 日本繁殖生物学会の論文
著者
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MANABE Noboru
Research Unit of Animal Life Sciences, Animal Resource Science Centre, The University of Tokyo
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Inoue Naoko
Laboratory Of Animal Morphology And Function Graduate School Of Bioagricultural Sciences Nagoya Univ
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MAEDA Akihisa
Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
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GOTO Yasufumi
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
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CHENG Yuan
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultu
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Matsuda-Minehata Fuko
Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo
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Matsuda Fuko
東京大学大学院農学生命科学研究科高等動物教育研究センター附属牧場
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Inoue Naoko
Unit Of Anatomy And Cell Biology Department Of Animal Sciences Kyoto University
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Manabe Noboru
Research Center For Food Safety Graduate School Of Agricultural And Life Sciences The University Of
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Maeda Akihisa
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Goto Yasufumi
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Matsuda-minehata Fuko
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Cheng Yuan
Research Unit For Animal Life Sciences Animal Resource Science Center Graduate School Of Agricultura
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Inoue Naoko
Laboratory Of Animal Morphology And Function Division Of Biofunctional Development Graduate School Of Bioagricultural Sciences Nagoya University
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INOUE Naoko
Laboratory of Animal Morphology and Function, Graduate School of Bioagricultural Sciences, Nagoya University
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