Experimental Evidence for the Influence of Cumulus-Oocyte-Complexes on Sperm Release from the Porcine Oviductal Sperm Reservoir
スポンサーリンク
概要
- 論文の詳細を見る
In the pig, a temporal relationship is suggested between sperm release from the sperm reservoir (SR) and ovulation, but the mechanism(s) is still under discussion. In two experiments, the influence of transferred ova on the release of SR-spermatozoa at ovulation and the effect of supplementation with non-sulfated glycosaminglycan hyaluronan (HA) on embryo development and the number of accessory spermatozoa, respectively, were examined. PMSG/hCG primed ovectomized gilts that had previously received endoscopic low-dose insemination into the cranial uterine horn were used as an experimental model. After salpingectomy, tubal segments (ampulla, cranial, and caudal isthmus) were flushed and sperm numbers or respective accessory spermatozoa were counted. In Experiment 1, the distribution of the sperm population was altered in the presence of cumulus-oocyte-complexes (COCs). A higher proportion of spermatozoa was found after transfer of COCs into one oviduct in the ampulla and cranial isthmus segments compared with the controls (17.5 vs. 4.9%, p<0.05). In Experiment 2, the quality of the transferred ova and treatment influenced the presence of accessory spermatozoa. Transfer of COCs together with HA increased (p<0.05) the number of accessory spermatozoa compared with the other treatment groups and was similar to those in the "undisturbed" controls. No modifications were obtained regarding mean blastomere numbers (2.6 ± 0.2 to 3.1 ± 0.2). In summary, this study was demonstrated that cumulus-oocyte-complexes may be involved in triggering sperm release from the pig oviductal SR and that HA might be related to sperm release.
- 日本繁殖生物学会の論文
著者
-
BRUSSOW Klaus-Peter
FBN Research Institute for the Biology of Farm Animals
-
TORNER Helmut
FBN Research Institute for the Biology of Farm Animals
-
RATKY Jozsef
Research Institute for Animal Breeding and Nutrition
-
TUCHSCHERER Armin
FBN Research Institute for the Biology of Farm Animals
-
Manabe Noboru
Research Center For Food Safety Graduate School Of Agricultural And Life Sciences The University Of
-
MANABE Noboru
Research Unit for Animal Life Sciences, Animal Resource Center, The University of Tokyo
関連論文
- Metabolic Conversion of Zearalenone to α-Zearalenol by Goat Tissues
- Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
- 異種移植したブタ卵巣組織における卵胞と卵母細胞の発育と成熟およびその体外成熟培養
- Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
- 経胎盤ビスフェノールA(BPA)暴露が器官形成期のマウス胎仔におけるarylhydrocarbon受容体(AhR)とレチノイン酸受容体mRNA発現におよぼす影響
- ブタ卵管の精子貯留部からの精子放出に卵子-卵丘細胞複合体が影響する
- ブタ卵胞顆粒層細胞膜上の抗原を認識するモノクローナル抗体
- ブタ卵胞閉鎖にともなうコネキシン43の発現と局在の推移
- Expression and Localization of cFLIP, an Anti-apoptotic Factor, in the Bovine Corpus Luteum
- Occurrence of Polyovular Follicles in Mouse Lines Selected for High Fecundity
- Sperm Migration in Pigs after Deep Intrauterine and Intraperitoneal Insemination
- ICGNマウスにおける組織トランスグルタミナーゼ(tTG)の局在
- cFLIP Regulates Death Receptor mediated Apoptosis in an Ovarian Granulosa Cell Line by Inhibiting Procaspase-8 Cleavage
- Changes in Expression of Interleukin-6 Receptors in Granulosa Cells During Follicular Atresia in Pig Ovaries
- The Regulation of Ovarian Granulosa Cell Death by Pro- and Anti-apoptotic Molecules
- Expression and Localization of Fas Ligand and Fas During Atresia in Porcine Ovarian Follicles
- Influence of the Dominant White/KIT Genotypes on the Reproductive Organs of Pigs
- The Role of Interleukin-6 in the Regulation of Granulosa Cell Apoptosis during Follicular Atresia in Pig Ovaries
- Molecular Characteristics of Porcine Fas-associated Death Domain (FADD) and Procaspase-8
- Comparison of Luteinizing Hormone, Leptin and Progensterone Levels in the Systemic Circulation (Vena jugularis) and near the Ovarian Circulation (Vena cava caudalis) during the Oestrous Cycle in Mangalica and Landrace Gilts
- ハンガリー在来ブタ(Mangalica)とLandrace雌ブタの排卵期における卵胞・卵母細胞の発育・成熟と生殖ホルモン分泌の比較
- Conflict of Estrogenic Activity by Various Phthalates between In Vitro and In Vivo Models Related to the Expression of Calbindin-D_
- Molecular Characteristics of Porcine Fas-associated Death Domain (FADD) and Procaspase-8
- Growth and Maturation of Follicles and Oocytes Following Xenotransplantation of Porcine Ovarian Tissues and In Vitro Maturation
- Changes in Expression and Localization of Connexin 43 mRNA and Protein in Porcine Ovary Granulosa Cells during Follicular Atresia
- Effects of Exposure In Utero to Bisphenol A on the Expression of Aryl Hydrocarbon Receptor, Related Factors, and Xenobiotic Metabolizing Enzymes in Murine Embryos
- Effects of In Utero Exposure to Bisphenol A on mRNA Expression of Arylhydrocarbon and Retinoid Receptors in Murine Embryos
- Porcine (Sus scrofa) Cellular FLICE-like Inhibitory Protein (cFLIP) : Molecular Cloning and Comparison with the Human and Murine cFLIP
- Regulation Mechanism of Selective Atresia in Porcine Follicles : Regulation of Granulosa Cell Apoptosis during Atresia
- Comparison of Follicular and Oocyte Development and Reproductive Hormone Secretion during the Ovulatory Period in Hungarian Native Breed, Mangalica, and Landrace Gilts
- Expression and Function of Apoptosis Initiator FOXO3 in Granulosa Cells During Follicular Atresia in Pig Ovaries
- Incorporation of β-amyloid protein through the bovine ileal epithelium before and after weaning : Model for orally transmitted amyloidoses
- Effect of Recombinant Bovine Somatotropin (rbST) on Cytoplasmic Maturation of Bovine Oocytes and Their Developmental Competence In Vitro
- 成雄ウシ由来線維芽細胞と成雌ウシ由来卵胞顆粒層細胞を用いた体細胞核移植におけるHandmade-Cloning法の有用性
- Sperm Migration in Pigs after Deep Intrauterine and Intraperitoneal Insemination
- Effect of Recombinant Bovine Somatotropin (rbST) on Cytoplasmic Maturation of Bovine Oocytes and Their Developmental Competence In Vitro
- Follicular Growth and Atresia in Mammalian Ovaries : Regulation by Survival and Death of Granulosa Cells
- Saving Genetic Resources of Native Pigs in Occidental and Oriental Countries — Practical Examples of the Characterization and Utilization of Native Pigs in Hungary and Laos
- Effects of Long-Term Grafting on Follicular Growth in Porcine Ovarian Cortical Grafts Xenoplanted to Severe Combined Immunodeficient (SCID) Mice
- Experimental Evidence for the Influence of Cumulus-Oocyte-Complexes on Sperm Release from the Porcine Oviductal Sperm Reservoir