Expression and Localization of cFLIP, an Anti-apoptotic Factor, in the Bovine Corpus Luteum
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概要
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The objective of the present study was to investigate the potential mechanisms regulating cellular FLICE-like inhibitory protein (cFLIP), an anti-apoptotic factor, in the bovine corpus luteum (CL). Expression of cFLIP mRNA was highest at the developing stage and then decreased significantly during the mid, late and regressed stages (P<0.05). Western blot analysis revealed that expression of the long isoform of cFLIP (cFLIPL) protein was high during the early and developing luteal stages, remained steady during the mid and late luteal stages and then decreased significantly (P<0.05) by the regressed stage. However, the expression levels of the short isoform of cFLIP (cFLIPS) remained low during the early, developing and mid luteal stages. Immunostaining of cFLIP was strongest in the cytoplasm of luteal and non-luteal cells, including endothelial and immune cells, remained high during the early, developing and mid luteal stages and then decreased significantly (P<0.05) in the late and regressed luteal stages. Immunostaining of cFLIP was observed only in macrophage-like cells in the regressing CL. However, cultured mid luteal cells had a higher percentage of cFLIP-positive cells and a lower percentage of TUNEL-positive cells than luteal cells treated with tumor necrosis factor α (TNF)/interferon γ (IFNG; P<0.01). These results indicate downregulation of cFLIP during structural luteal regression, suggesting that cFLIP plays a survival role in the bovine CL.
- 2010-04-01
著者
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MANABE Noboru
Research Unit of Animal Life Sciences, Animal Resource Science Centre, The University of Tokyo
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OKUDA Kiyoshi
Laboratory of Reproductive Endocrinology, Okayama University
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ACOSTA Tomas
Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama
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Okuda Kiyoshi
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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HOJO Takuo
Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama
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KOMIYAMA Junichi
Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama
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Acosta Tomas
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Manabe Noboru
Research Unit For Animal Life Sciences Animal Resource Science Center The University Of Tokyo
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Komiyama Junichi
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Hojo Takuo
Lab. Of Reproductive Endocrinology Graduate School Of Natural Sci. And Technol. Okayama Univ.
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Hojo Takuo
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Manabe Noboru
Research Center For Food Safety Graduate School Of Agricultural And Life Sciences The University Of
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Okuda Kiyoshi
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Acosta Tomas
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Al-zl'abi Mohamad
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Hojo Takuo
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Al-Ziabi Mohamad
Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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ACOSTA Tomas
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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