Biological Activity of Recombinant Bovine Interferon τ Produced by a Silkworm-Baculovirus Gene Expression System
スポンサーリンク
概要
- 論文の詳細を見る
Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vitro development of bovine embryos. Bm-rbIFNτ and OT were shown to suppress PGF2α production in a dose-dependent manner. When in vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa medium supplemented with or without rbIFNτ, Bm-rbIFNτ (10 ng/ml) significantly promoted development to the expanded blastocyst stage. In conclusion, Bm-rbIFNτ was suggested to have the same bioactivity as native IFNτ.
- 公益社団法人 日本獣医学会の論文
著者
-
Okuda Kiyoshi
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
-
INUMARU Shigeki
National Institute of Animal Health
-
Takahashi Masashi
Livestock And Grassland Res. Div. Kyushu Okinawa Agricultural Res. Center National Agriculture And Food Res. ...
-
HIRAKO Makoto
Animal Breeding and Reproduction Research Division, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)
-
TAKAHASHI Hitomi
Animal Breeding and Reproduction Research Division, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)
-
TSUNAZAKI Makoto
Animal Breeding and Reproduction Research Division, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)
-
HAMANO Takashi
Animal Breeding and Reproduction Research Division, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)
-
OKANO Akira
Animal Breeding and Reproduction Research Division, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)
-
GESHI Masaya
Animal Breeding and Reproduction Research Division, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)
-
INUMARU Shigeki
National Institute of Animal Health, National Agriculture and Food Research Organization
-
TAKAHASHI Masashi
Livestock and Grassland Research Division, NARO Kyushu Okinawa Agricultural Research Center
関連論文
- Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia: The Role of Follicular Stimulating Hormone
- Changes in the Levels of Progesterone Receptor mRNA and Protein in the Bovine Corpus Luteum During the Estrous Cycle
- Expression of VEGF and Its Receptors in the Bovine Endometrium Throughout the Estrous Cycle : Effects of VEGF on Prostaglandin Production in Endometrial Cells
- 牛初期胚発育に及ぼすインターフェロンτの作用
- Role of Nitric Oxide in the Regulation of Superoxide Dismutase and Prostaglandin F_ Production in Bovine Luteal Endothelial Cells
- 日本繁殖生物学会賞・奨励賞 卵巣機能調節機構における腫瘍壊死因子の生理的役割
- ウシ顆粒層細胞および卵胞膜内膜細胞における腫瘍壊死因子(TNFα)受容体
- Expression and Localization of cFLIP, an Anti-apoptotic Factor, in the Bovine Corpus Luteum
- Species-Related Differences in the Mechanism of Apoptosis During Structural Luteolysis
- Luteotropic Mechanisms in the Bovine Corpus Luteum : Role of Oxytocin, Prostaglandin F_, Progesterone and Noradrenaline
- Acute Changes in Circulating Concentrations of Progesterone and Nitric Oxide and Partial Pressure of Oxygen During Prostaglandin F_-induced Luteolysis in Cattle
- Changes in the Vasculature of Bovine Corpus Luteum During the Estrous Cycle and Prostaglandin F2α-induced Luteolysis
- Possible Roles of Intracellular Cyclic AMP, Protein Kinase C and Calcium Ion in the Apoptotic Signaling Pathway in Bovine Luteal Cells
- Prostaglandin F_ Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells
- 正常発情周期中のブタ子宮内膜ではトロフィニンが発現している
- Expression of Equine Interleukin-18 by Baculovirus Expression System and Its Biologic Activity
- Nitric oxide in bovine corpus luteum : Possible mechanisms of action in luteolysis
- Phyto- and Endogenous Estrogens Differently Activate Intracellular Calcium Ion Mobilization in Bovine Endometrial Cells
- Hypoxia is Important for Establishing Vascularization During Corpus Luteum Formation in Cattle
- Regulation of uterine function by cytokines in cows : Possible actions of tumor necrosis factor-α, interleukin-1α and interferon-τ
- Hypoxia is Important for Establishing Vascularization During Corpus Luteum Formation in Cattle
- Gene Silencing of Cyclooxygenase-2 mRNA by RNA Interference in Bovine Cumulus-Granulosa Cells
- Effects of Prostaglandin F_ and Nitric Oxide on the Secretory Function of Bovine Luteal Cells
- Effects of Nitric Oxide and Tumor Necrosis Factor-α on Production of Prostaglandin F_ and E_2 in Bovine Endometrial Cells
- Progesterone Stimulation by LH Involves the Phospholipase-C Pathway in Bovine Luteal Cells
- Age-Related Changes in the Serum Levels of Inhibin, FSH, LH and Testosterone in Holstein Bulls
- Basic Fibroblast Growth Factor-Induced Prostaglandin Production and Its Intracellular Mechanisms in Cultured Bovine Luteal Cells
- MECHANISM IN THE REGULATION OF OVARIAN CYCLE : REGULATION OF ENDOMETRIAL PROSTAGLANDIN F_ SYNTHESIS IN LUTEOLYSIS IN CATTLE
- Expressions of Estrogen Receptors in the Bovine Corpus Luteum : Cyclic Changes and Effects of Prostaglandin F2α and Cytokines
- Role of Nitric Oxide in the Regulation of Superoxide Dismutase and Prostaglandin F2α Production in Bovine Luteal Endothelial Cells
- Expression and Characterization of Bluetongue Virus Serotype 21 VP7 Antigen: C-Terminal Truncated Protein Has Significantly Reduced Antigenicity
- ウシ子宮内膜の上皮細胞および間質細胞の単離・継代・凍結保存法の開発
- Effects of Tumor Necrosis Factor α and Interferon γ on the Viability and mRNA Expression of TNF Receptor Type I in Endothelial Cells from the Bovine Corpus Luteum
- Progesterone Stimulation by Prostaglandin F_ Involves the Protein Kinase C Pathway in Cultured Bovine Luteal Cells
- Secretion of Prostaglandins E_2 and F_ by the Bovine Endometrium in Response to Oxytocin during the Estrous Cycle
- Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone
- Effects of Storage and Passage of Luteal Endothelial Cells on Endothelin-1 and Prostaglandin F2α Production
- Oxidative Stress and Redox Regulation on In Vitro Development of Mammalian Embryos
- Effects of Cortisol on Pregnancy Rate and Corpus Luteum Function in Heifers : An In Vivo Study
- Seasonal Changes in Luteal Progesterone Concentration and mRNA Expressions of Progesterone Synthesis-related Proteins in the Corpus Luteum of Mares
- Seasonal Changes in Luteal Progesterone Concentration and mRNA Expressions of Progesterone Synthesis-related Proteins in the Corpus Luteum of Mares
- Possible Role of Insulin-Like Factor 3 in the Bovine Corpus Luteum
- Stability of recombinant bovine interferon-γ antiviral activity in the absence of stabilizing additives
- Luteoprotective Mechanisms of Prostaglandin F2α Stimulated by Luteinizing Hormone in the Bovine Corpus Luteum
- Induction of the Expressions of Antioxidant Enzymes by Luteinizing Hormone in the Bovine Corpus Luteum
- Acute Changes in Circulating Concentrations of Progesterone and Nitric Oxide and Partial Pressure of Oxygen During Prostaglandin F2.ALPHA.-induced Luteolysis in Cattle
- Biological Activity of Recombinant Bovine Interferon τ Produced by a Silkworm-Baculovirus Gene Expression System
- Expression of Equine Interleukin-18 by Baculovirus Expression System and Its Biologic Activity