Prostaglandin F_<2α> Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells
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概要
- 論文の詳細を見る
The objective of the present study was to elucidate whether luteolytic prostaglandin F2α (PGF) plays roles in regulating the nitric oxide (NO) generating system in luteal endothelial cells (LECs). Reverse transcriptase PCR, immunoblotting and immuno- staining revealed the presence of PGF receptor mRNA (521 bp) and protein (64 kDa) in cultured LECs obtained from the mid-stage corpus luteum. When cultured LECs were exposed to 0.1 μM-10 μM PGF, NO production was significantly stimulated by PGF at 24 h. When LECs were exposed to 1 μM PGF for 2, 6 and 24 h, PGF did not affect the expressions of endothelial NO synthase (eNOS) mRNA and protein. On the other hand, PGF stimulated the expression of inducible NOS (iNOS) mRNA (P<0.05) and protein (P<0.05) at 2 h, but not at 6 and 24 h. By observing the conversion of [3C] L-arginine to [3C]L-citrulline, we found that PGF stimulated NOS activity in cultured LECs at 2 h (P<0.05). The overall findings indicate that bovine LECs are a target for PGF and that PGF stimulates iNOS expression and NOS activity in bovine LECs. Stimulation of the NO generating system and NOS activity by PGF may result in increasing local NO production followed by luteolysis.
- 日本繁殖生物学会の論文
- 2009-08-01
著者
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OKUDA Kiyoshi
Laboratory of Reproductive Endocrinology, Okayama University
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ACOSTA Tomas
Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama
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Okuda Kiyoshi
Lab. Of Reproductive Endocrinology Graduate School Of Natural Sci. And Technol. Okayama Univ.
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Okuda Kiyoshi
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Acosta Tomas
Lab. Of Reproductive Endocrinology Graduate School Of Natural Sci. And Technol. Okayama Univ.
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Acosta Tomas
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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アコスタ トマスj
帯広畜産大学・畜産管理学科
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奥田 潔
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Acosta Tomas
Graduate School Of Natural Science And Technology Okayama University
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Lee Seung-Hyung
Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama
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YOSHIOKA Shin
Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama
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奥田 潔
岡山大学大学院自然科学研究科バイオサイエンス専攻生殖内分泌学
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Yoshioka Shin
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Okuda Kiyoshi
Okayama Univ. Okayama
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Acosta T
Department Of Theriogenology Obihiro University Of Agriculture And Veterinary Medicine
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Lee Seung-hyung
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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Yoshioka Shin
Laboratory Of Drug Metabolism & Toxicology Department Of Hygienic Chemistry Tokyo College Of Pha
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ACOSTA Tomas
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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