ウシ成熟卵胞を微透析した灌流液中におけるGnRH-immunoreactivityの存在

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概要

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• The presence of gonadotropin-releasing hormone (GnRH)-like protein has been previously demonstrated in the bovine follicle. This GnRH-like protein was purified and concluded to be histone H2A. However, neither GnRH peptide nor specific GnRH-immunoreactivity (GnRH-IR) has been demonstrated in the bovine follicle so far. Thus, this study focused on the detection of specific GnRH-IR using the second-antibody enzyme immunoassay in isolated bovine mature follicles, and on an examination of the direct effect of luteinizing hormone (LH), endothelin-1 (ET-1), and cytokines on the GnRH-IR using an in vitro microdialysis system (MDS). We further examined a cross-reactivity of the GnRH antibody with bovine histone H2A. GnRH-IR was detected in microdialyzed perfusate from isolated bovine mature follicles at 4.40 ± 0.35 pg/ml (mean ± SEM). Bovine histone H2A showed no GnRH-IR at all. Heat treatment of the extract (100 C for 10 min) did not affect the GnRH-IR. Single infusion of LH, ET-1, or cytokines into the MDS did not affect the GnRH-IR. However, infusion of ET-1 after LH exposure increased the GnRH-IR. These results demonstrate the presence of specific GnRH-IR, that is different from histone H2A, in microdialyzed perfusate of isolated bovine mature follicles in vitro, suggesting that the GnRH-IR may reflect a role of GnRH-like peptide or some peptide structurally similar to GnRH in the local regulation of mature follicles.

• 日本繁殖生物学会の論文
• 2000-04-01

著者

• NAKAMURA Tadashi

  Department of Metabolic Medicine, Osaka University Graduate School of Medicine

• Acosta Tomas

  Lab. Of Reproductive Endocrinology Graduate School Of Natural Sci. And Technol. Okayama Univ.

• アコスタ トマスj

  帯広畜産大学・畜産管理学科

• MIYAMOTO Akio

  Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medici

• Acosta Tomas

  Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine

• OZAWA Takahiro

  Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine

• Acosta Tomas

  Graduate School Of Natural Science And Technology Okayama University

• Ozawa T

  Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn

• Nakamura Tadashi

  Department Of Metabolic Medicine Osaka University Graduate School Of Medicine

• Miyamoto A

  Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi

• Miyamoto Akio

  Department Of Animal Science Obihiro University Of Agriculture And Veterinary Medicine

• Acosta T

  Department Of Theriogenology Obihiro University Of Agriculture And Veterinary Medicine

• Miyamoto Akio

  Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin

• Nakamura Tadashi

  Department Of Anesthesia Junwakai Memorial Hospital

• Miyamoto Akio

  Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Veterinary Medicine

• Nakamura Tadashi

  Department Of Bioresourse Science Obihiro University Of Agriculture And Veterinary Medicine

• ACOSTA Tomas

  Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine

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