生体微透析システムを用いてウシ黄体内に灌流したPGF2α、TPAおよびカルシウムイオノフォアA23187が黄体のProgesterone放出に及ぼす直接的影響
スポンサーリンク
概要
- 論文の詳細を見る
There is general agreement that prostaglandin F2α (PGF2α) is a physiological luteolysin in the cow. Contrary to this fact, PGF2α has been shown to stimulate the progesterone (P) release from luteal cells in vitro, through the activation of intracellular protein kinase C (PKC) and free calcium ion (Ca2+). To examine the possibility that an essential factor or function(s) may be lost through the process of preparing cells from the corpus luteum (CL), we evaluated the direct effect of PGF2α, TPA and ionophore A23187 on local P release within the CL in the cow, by utilizing in vivo microdialysis system (MDS). Normal cyclic Holstein cows (n=4) were superovulated with FSH and the PGF2α analogue, and on Day 8 after estrus, they were surgically implanted with the MDS (cut-off Mr=1000 kDa) into multiple CL (1-3 line/CL). The system was continuously perfused with Ringers solution from immediately after the surgery. On Days 10-11, several CL were assigned to the control, PGF2α (10-5 M), TPA (10-5 M), A23187 (10-5 M), PGF2α+A23187 and TPA+A23187. The stimulants were infused into the CL using the MDS for 6 h, and each line of the MDS was further perfused with Ringers solution for 26 h. A 6-h infusion with PGF2α stimulated P release during infusion, but after infusion the P release was again stable around the baseline. A 6-h infusion with TPA or A23187 strongly stimulated the P release. Similarly, PGF2α+A23187 or TPA+A23187 stimulated the P release. During the last 8 h of the experiment, A23187 alone, PGF2α+A23187 or TPA+A23187 inhibited the P release, whereas TPA as well as PGF2α maintained the same level as in the control. The results indicate that the direct exposure of the microenvironment in the bovine CL to PGF2α in vivo does not result in the suppression of P release until 26 h after stimulation, and that the activation of cytosolic free calcium is more effective in inhibiting the P release than the stimulation of PKC during the following period. Thus, the results support the concept that PGF2α needs some local luteolytic intermediator(s) that may enhance the intracellular calcium concentration.
- 日本繁殖生物学会の論文
- 1999-06-01
著者
-
小林 修一
帯広畜産大学・畜産科学科・生殖科学研究室
-
宮本 明夫
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080
-
大谷 昌之
University Farm
-
OHTANI Masayuki
University Farm, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080, Japan
-
Amaya Montoya
帯広畜産大学 畜産学研究科畜産衛生学
-
Kobayashi Shu-ichi
Department Of Animal Science Obihiro University Of Agriculture And Veterinary Medicine
-
Ohtani M
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn
-
Miyamoto A
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
-
Miyamoto Akio
Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin
-
小林 修一
Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin
-
Miyamoto Akio
Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Veterinary Medicine
-
Miyamoto Akio
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
-
Kobayashi S
Obihiro University Of Agriculture And Veterinary Medicine
-
Kobayashi S
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn
関連論文
- Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia: The Role of Follicular Stimulating Hormone
- Classification of Bovine Follicles Based on the Concentrations of Steroids, Glucose and Lactate in Follicular Fluid and the Status of Accompanying Follicles
- Vascular Changes in the Corpus Luteum During Early Pregnancy in the Cow
- Prostaglandin F_ Differentially Affects mRNA Expression Relating to Angiogenesis, Vasoactivation and Prostaglandins in the Early and Mid Corpus Luteum in the Cow
- ウシ成熟卵胞内におけるGnRH投与による排卵時期の Fibroblast Growth Factor 1 (FGF1) およびFGF7遺伝子発現の変化
- eCG/hCG処理した未成熟雌マウスの卵巣におけるPin1(ペプチジルプロピルイソメラーゼ)の発現
- ニホンジカ (Cervus nippon) 成熟雄における血清LHおよびテストステロン濃度の周年変化
- ウシ生体を用いた黄体形成期における黄体内のプロスタグランジン(PG)F2αおよびPGE2濃度の経時的変化
- MDSを用いた馬主席卵胞のLHとPGF_感作試験(臨床繁殖学)
- 雌仔ウシおよび成熟雌ウシの末梢血中プロジェステロンとエンドセリン濃度の変化 : PGF_投与の影響
- 磁気誘導薬剤配送システムを用いた磁性薬剤の血管内集積に関する研究
- ウシ生体を用いた黄体形成期における黄体内のプロスタグランジン(PG)F2αおよびPGE2濃度の経時的変化
- ウシ黄体化顆粒層細胞におけるアンギオテンシン2の生産および分泌機能への作用の欠如
- Angiotensin II Secretion by the Bovine Oviduct is Stimulated by Luteinizing Hormone and Ovarian Steroids
- Distribution of Arteriolovenous Vessels, Capillaries and eNOS Expression in the Bovine Corpus Luteum During the Estrous Cycle : a Possible Implication of Different Sensitivity by Luteal Phase to PGF_ in the Increase of Luteal Blood Flow
- A novel concept for the bovine luteolytic cascade with a focus on luteal blood flow and vasoactive factors
- Effect of Suckling on the Reproductive Performance and Metabolic Status of Obese Japanese Black Cattle during the Early Postpartum Period
- Local mechanisms for luteolysis in the cow : Novel roles of vasoactive substances in the luteolytic cascade within the corpus luteum
- Metabolic Profiles in Ovulatory and Anovulatory Primiparous Dairy Cows during the First Follicular Wave Postpartum
- 性成熟時および性成熟後のホルスタイン種雄牛における月齢,体重,精巣周囲長,精液性状および血中テストステロンとエストラジオール-17β濃度の関連性(臨床繁殖学)
- Genital Blood Flow and Endometrial Gene Expression During the Preovulatory Period after Prostaglandin F_-Induced Luteolysis in Different Luteal Phases in Cows
- Distribution of Arteriolovenous Vessels, Capillaries and eNOS Expression in the Bovine Corpus Luteum During the Estrous Cycle: a Possible Implication of Different Sensitivity by Luteal Phase to PGF2 α in the Increase of Luteal Blood Flow
- Changes in the Messenger RNA Expressions of the Endothelin-1 and Angiotensin Systems in Mature Follicles of the Superovulated Bovine Ovary
- Electroplated Reflection Masks for Soft X-Ray Projection Lithography
- Resist Performance in 5 nm Soft X-Ray Projection Lithography
- Reduction Imaging at 4.5 nm with Schwarzschild Optics
- Fabrication of 0.1μm Line-and-Space Patterns using Soft X-Ray Reduction Lithography
- Improvement of the Uniformity of Tungsten / Carborn Multilayers by Thermal Processing
- Sub-0.1 μm Resist Patterning in Soft X-Ray (13 nm) Projection Lithography
- Normal-Incidence X-Ray Microscope for Carbon Kα Radiation with 0.5 μm Resolution
- ウシ形成期黄体において、プロスタグランジンF2αはプロジェステロン生成のためのリポプロテイン利用性を増加させる
- 生体微透析システムを用いてウシ黄体内に灌流したPGF2α、TPAおよびカルシウムイオノフォアA23187が黄体のProgesterone放出に及ぼす直接的影響
- Colour Doppler Sonography of Cystic Ovarian Follicles in Cows
- Effect of Intraluteal Injection of Endothelin Type A Receptor Antagonist on PGF_-induced Luteolysis in the Cow
- Tumor Necrosis Factor α System in the Bovine Oviduct : A Possible Mechanism for Embryo Transport
- Comparative Activities of Growth Hormone and Luteinizing Hormone in the Direct Stimulation of Local Release of Progesterone from Microdialyzed Ovine Corpora Lutea In Vivo
- Effective Oxytocin Treatment on Placental Expulsion after Foaling in Heavy Draft Mares(Theriogenology)
- Relationship between Peripartal Plasma Oxytocin and Prostaglandin F_ Metabolite and Placental Expulsion Time in Heavy Draft Mares
- A Potential Use of Color Ultrasound as a Tool for Reproductive Management : New Observations Using Color Ultrasound Scanning that were not Possible with Imaging Only in Black and White
- Relationship between the First Ovulation within Three Weeks Postpartum and Subsequent Ovarian Cycles and Fertility in High Producing Dairy Cows
- Subjecting Holstein Heifers to Stress During the Follicular Phase Following Superovulatory Treatment May Increase the Female Sex Ratio of Embryos
- Prostaglandin F2α Differentially Affects mRNA Expression Relating to Angiogenesis, Vasoactivation and Prostaglandins in the Early and Mid Corpus Luteum in the Cow
- Expression of mRNA for Cell Adhesion Molecules in the Bovine Corpus Luteum During the Estrous Cycle and PGF_-Induced Luteolysis
- Induction of Ovulation with GnRH and PGF_ at Two Different Stages During the Early Postpartum Period in Dairy Cows : Ovarian Response and Changes in Hormone Concentrations
- 生後性成熟過程のウシ潜在精巣におけるα-SMAおよび vimentin の免疫組織化学的局在パターンの異常
- ウシにおいて排卵前の卵胞内でコルチゾールの局所産生が増加する生体内での証拠
- 乳牛の第1卵胞波における卵胞への血流の変化
- ウシ卵管上皮細胞が分泌する可溶型N-アセチルグルコサミニルトランスフェラーゼ1:LH,VEGFおよびTNFαによる分泌刺激効果
- 雌仔ウシおよび成熟雌ウシの末梢血中プロジェステロンとエンドセリン濃度の変化:PGF2α投与の影響
- The Glauber Approximation and Evaluation of Ionization Cross Section
- Multiple Scattering Amplitude of the Ionization by Charged Particles in the Intermediate Energy Region
- ウシ黄体の形成と退行 : 古くて新しい血管と黄体機能の関係
- 1999年度島村賞受賞講演論文 Paracrine/autocrine機構による反芻家畜の卵胞・黄体機能調節に関する研究
- Differential Effect of Follicle-Stimulating Hormone and Estradiol on Expressions of Vascular Endothelial Growth Factor (VEGF) 120, VEGF164 and Their Receptors in Bovine Granulosa Cells
- Testicular Blood Flow and Plasma Concentrations of Testosterone and Total Estrogen in the Stallion after the Administration of Human Chorionic Gonadotropin
- 非繁殖期における2種類の膣内挿入器具を用いた雌羊の発情誘起と受胎性の比較
- Comparison of Estrus Induction and Subsequent Fertility with Two Different Intravaginal Devices in Ewes during the Non-Breeding Season
- Changes in Plasma Metabolic Hormone Concentrations During the Ovarian Cycles of Japanese Black and Holstein Cattle
- In Vivo Evidence that Local Cortisol Production Increases in the Preovulatory Follicle of the Cow
- Relationship between Serum Sex Hormone Concentrations and Histology of Seminiferous Tubules of Captured Baleen Whales in the Western North Pacific during the Feeding Season
- Metabolic Profiles in Ovulatory and Anovulatory Primiparous Dairy Cows During the First Follicular Wave Postpartum
- Production of Trophoblastic Vesicles Derived From Day 7 and 8 Blastocysts of In Vitro Origin and the Effect of Intrauterine Transfer on the Interestrous Intervals in Japanese Black Heifers
- ウシ成熟卵胞を微透析した灌流液中におけるGnRH-immunoreactivityの存在
- Changes in Follicular Vascularity during the First Follicular Wave in Lactating Cows
- 発情周期中およびプロスタグランジンF2α投与後のウシ黄体内でのアンジオポエチンと受容体TieのmRNA発現の変化:黄体退行開始機構への関与の可能性
- ウシ卵胞発育中の顆粒層細胞におけるアンジオポエチン-Tie系のmRNA発現
- Comparative Activities of Growth Hormone and Luteinizing Hormone in the Direct Stimulation of Local Release of Progesterone from Microdialyzed Ovine Corpora Lutea In Vivo
- 不妊発情周期における周期回帰のメカニズム
- Local Release of Progesterone and Oxytocin from Microdialyzed Corpus Luteum in Superovulated Ewes : Characterization during the Non-breeding Season
- A Simple Superovulation Method of A Single Injection of Follicle-Stimulating Hormone Combined with Equine Chorionic Gonadotropin for Superovulation of Suffolk Ewes During the Breeding Season : II. The Ovarian Responses and the Embryo Qualities
- A Simple Superovulation Method of A Single Injection of Follicle-Stimulating Hormone Combined with Equine Chorionic Gonadotropin for Superovulation of Suffolk Ewes During the Breeding Season : I. Effects of Different Treatments on the Endocrine Profiles
- Relationships Among Follicular Fluid Estradiol-17β Concentration, Morphology of Cumulus-Oocyte Complex and Developmental Capacity of Individually Matured, Fertilized and Cultured Bovine Oocytes In Vitro
- Exposure of C57BL/6J Male Mice to an Electric Field Improves Copulation Rates with Superovulated Females
- 非繁殖季節に発情を誘起したヒツジにおいて人工受精後11日目に投与したウシ組換え成長ホルモンの影響
- ウシ形成期黄体において、プロスタグランジンF2αはプロジェステロン生成のためのリポプロテイン利用性を増加させる
- Relationships Between the First Ovulation Postpartum and Polymorphism in Genes Relating to Function of Immunity, Metabolism and Reproduction in High-producing Dairy Cows
- 高泌乳牛の泌乳ピークと持続性が繁殖機能に及ぼす影響
- Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone
- Intraluteal Release of Progesterone and Prostaglandins during PGF_-Induced Luteolysis in Ewes : Local Effects of Tumor Necrosis Factor-α
- N-acetylglucosaminyltransferase I Activity of Bovine Oviduct Epithelial Cells : Stimulation by Luteinizing Hormone, Vascular Endothelial Growth Factor and Tumor Necrosis Factor α
- Detection of Zearalenone and Its Metabolites in Naturally Contaminated Porcine Follicular Fluid by Using Liquid Chromatography-Tandem Mass Spectrometry
- Changes in Plasma Metabolic Hormone Concentrations during the Ovarian Cycles of Japanese Black and Holstein Cattle
- 生体微透析システムを用いてウシ黄体内に灌流したPGF2α、TPAおよびカルシウムイオノフォアA23187が黄体のProgesterone放出に及ぼす直接的影響
- ウシ黄体化顆粒層細胞におけるアンギオテンシン2の生産および分泌機能への作用の欠如
- Basal Levels and GnRH-induced Responses of Peripheral Testosterone and Estrogen in Holstein Bulls with Poor Semen Quality
- Slow Recovery of Blood Glucose in Insulin Tolerance Test during the Prepartum Transition Period Negatively Impacts the Nutritional Status and Reproductive Performance Postpartum of Dairy Cows
- Upregulation of Interferon-stimulated Genes and Interleukin-10 in Peripheral Blood Immune Cells During Early Pregnancy in Dairy Cows
- Upregulation of Interferon-stimulated Genes and Interleukin-10 in Peripheral Blood Immune Cells During Early Pregnancy in Dairy Cows
- Nutritional Factors That Regulate Ovulation of the Dominant Follicle During the First Follicular Wave Postpartum in High-producing Dairy Cows
- Differential Effect of Follicle-Stimulating Hormone and Estradiol on Expressions of Vascular Endothelial Growth Factor (VEGF) 120, VEGF164 and Their Receptors in Bovine Granulosa Cells
- Gene Expressions in the Persistent Corpus Luteum of Postpartum Dairy Cows : Distinct Profiles from the Corpora Lutea of the Estrous Cycle and Pregnancy
- Effect of the Dominant Follicle Aspiration before or after Luteinizing Hormone Surge on the Corpus Luteum Formation in the Cow
- Regulation of Innate Immune Function in Bovine Oviduct Epithelial Cells in Culture: The Homeostatic Role of Epithelial Cells in Balancing Th1/Th2 Response
- Downregulation of Lymphatic Vessel Formation Factors in PGF2α-induced Luteolysis in the Cow
- Areolae of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis)
- Structure and Steroidogenesis of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis)
- Lipopolysaccharide (LPS) Inhibits Steroid Production in Theca Cells of Bovine Follicles In Vitro: Distinct Effect of LPS on Theca Cell Function in Pre- and Post-selection Follicles
- Structure and Steroidogenesis of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis)