Upregulation of Interferon-stimulated Genes and Interleukin-10 in Peripheral Blood Immune Cells During Early Pregnancy in Dairy Cows
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概要
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In cows, interferon-tau (IFNT) regulates maternal recognition around days 15-19 after artificial insemination (AI). The present study hypothesized that if key target genes of IFNT are clearly upregulated in earlier stages of pregnancy, these genes could be use as indices of future pregnancy in cows. Therefore, we determined the expression of these genes in peripheral blood mononuclear leukocytes (PBMCs) and polymorphonuclear granulocytes (PMNs) during the maternal recognition period (MRP). Twenty multiparous Holstein cows were subjected to AI on day 0 and categorized into the following groups: pregnancy (Preg, n=9), embryonic death (ED, n=5) and non-pregnancy (NP, n=6). Progesterone levels in the Preg group were higher than those in the NP group on days 12-21. ISG15 and OAS-1 (IFN-stimulated genes: ISGs) mRNA in PBMCs on day 8 was higher in the Preg group than in the NP group, and these mRNAs in PMNs was higher in the Preg group on day 5 than in the NP and ED groups. Interleukin-10 (IL-10, Th2 cytokine) mRNA expression increased on day 8 in the PBMCs of pregnant cows. Tumor necrosis factor α (TNF α, Th1 cytokine) mRNA expression was stable in all groups. In an in vitro cell culture experiment, IFNT stimulated mRNA expression of ISGs in both PBMCs and PMNs. IFNT stimulated IL-10 mRNA expression in PBMCs, whereas IFNT increased TNFα mRNA levels in PBMCs in vitro. The results suggest that ISGs and IL-10 could be responsive to IFNT before the MRP in peripheral blood immune cells and may be useful target genes for reliable indices of pregnancy before the MRP.
著者
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Sasahara Kiemi
帯広畜産大学 大学院畜産衛生学
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Shimizu Takashi
Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Kida Katsuya
Field Center Of Animal Science And Agriculture Obihiro University Of Agriculture And Veterinary Medi
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Shirasuna Koumei
Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Veterinary Medicine
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Matsui Motozumi
Department Of Clinically Veterinary Sciences Obihiro University Of Agriculture And Veterinary Medici
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Miyamoto Akio
Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Veterinary Medicine
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Kawashima Chiho
Field Center Of Animal Sci. Obihiro Univ. Of Agriculture And Veterinary Medicine Hokkaido 080-8555 J
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MATSUMOTO Haruka
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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KOBAYASHI Eiji
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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NITTA Akane
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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HANEDA Shingo
Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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Kobayashi Eiji
Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Matsui Motozumi
Department Of Clinical Veterinary Science Obihiro University Of Agriculture And Veterinary Medicine
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Haneda Shingo
Department Of Clinical Veterinary Science Obihiro University Of Agriculture And Veterinary Medicine
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Nitta Akane
Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Matsumoto Haruka
Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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KIDA Katsuya
Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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SHIRASUNA Koumei
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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KAWASHIMA Chiho
Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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MIYAMOTO Akio
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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MATSUI Motozumi
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine
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SHIMIZU Takashi
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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