Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia: The Role of Follicular Stimulating Hormone

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概要

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• Glucocorticoids modulate ovarian function in cattle. However, their regulatory mechanisms have not been fully elucidated. In the present study, we examined gene expression of two glucocorticoid-metabolizing enzymes, a bidirectional 11β-HSD type 1 (11HSD1) and a dehydrogenase 11β-HSD type 2 (11HSD2), and glucocorticoid receptor (GR) in bovine follicles during follicular maturation and atresia. Granulosa cells (GCs) and theca interna layers (TIs) were harvested from follicles classified as small growing, dominant, preovulatory, early atretic and late atretic follicles. The expression levels of 11HSD1, 11HSD2 and GR mRNA were quantified by real-time PCR. In the healthy follicles, expression of 11HSD1 mRNA increased as follicles matured, both in GCs and TIs. A significant negative correlation was found between the concentration of cortisol in follicular fluid and the level of 11HSD1 mRNA in GCs. The expression of 11HSD2 and GR was either very low or largely unchanged during follicular maturation. In the atretic follicles, a drastic increase in the expression of 11HSD2 was observed both in GCs and TIs. To assess the effect of FSH on the expression of 11HSDs and GR, GCs were cultured with FSH (0-100 ng/ml) for up to 6 days. FSH increased 11HSD1 mRNA in a dose-dependent manner, but not 11HSD2, nor GR. Taken together, these results suggest that developmentally-regulated 11HSD1 plays a pivotal role in modulating the local glucocorticoid environment in maturing bovine follicles.

著者

• OKUDA Kiyoshi

  Laboratory of Reproductive Endocrinology, Okayama University

• HAMANO Seizo

  Animal Bio-Technology Center, Livestock Improvement Association of Japan, Inc.

• TETSUKA Masafumi

  Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medici

• Nishimoto Hiromi

  Department of Agricultural and Life Science, Obihiro University of Agriculture & Veterinary Medicine

• Miyamoto Akio

  Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine

• Tetsuka Masafumi

  Department Of Life Science And Agriculture Obihiro University Of Agriculture And Veterinary Medicine

• Tetsuka Masafumi

  Department Of Agriculture And Life Science Obihiro University Of Agriculture And Veterinary Medicine

• Tetsuka Masafumi

  Dep. Of Agricultural And Life Sci. Obihiro Univ. Of Agriculture And Veterinary Medicine

• Okuda Kiyoshi

  Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U

• Nishimoto Hiromi

  Department Of Life Science And Agriculture Obihiro University Of Agriculture And Veterinary Medicine

• Hamano Seizo

  Animal Biotechnology Center Livestock Improvement Association Of Japan

• Miyamoto Akio

  Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine

• Amaya Montoya

  帯広畜産大学 畜産学研究科畜産衛生学

• Miyamoto A

  Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi

• Hamano Seizo

  Animal Bio-technology Center Livestock Improvement Association Of Japan Inc.

• Tetsuka Masafumi

  Department Of Agricultural And Life Science Obihiro University Of Agriculture & Veterinary Medic

• Nishimoto Hiromi

  Department Of Agricultural And Life Science Obihiro University Of Agriculture & Veterinary Medic

• Miyamoto Akio

  Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Veterinary Medicine

• Miyamoto Akio

  Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi

• Hamano Seizo

  Animal Bio-technology Center Livestock Improvement Association

• Nishimoto Hiromi

  Department of Agricultural and Life Science, Obihiro University of Agriculture & Veterinary Medicine

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