Developmental Capacity of F1 Hybrid Embryos Produced by In Vitro Fertilization between Different Breeds of Japanese Beef Cattle

概要

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The objective of this study was to compare in vitro development to the blastocyst stage of bovine embryos obtained by in vitro fertilization within a breed and between different breeds of cattle. The timing of maturaion division did not differ among oocytes of Japanese Black (JB), Japanese Brown (JBr) and Japanese Shorthorn (JS); 91.5 to 97.5% of the oocytes were at the second metaphase after 21 h of culture. The rate of development to the blastocyst stage was also similar (34.6 to 37.5%) among three kinds of embryos (JB, JBr and JS) that were fertilized in vitro and cultured for 6 to 8 days after insemination. However, significantly higher rates of development to the blastocyst stage were observed (55.0 to 67.2%) when spermatozoa from a JB bull were used for in vitro fertilization of JBr, JS and JP (Japanese Polled) oocytes as compared to the rate of development in control JB embryos (36.7%). Development to the 2- to 4-cell stage did not differ significantly. These results suggest that the effect of heterosis becomes apparent at the blastocyst stage, which might be under the control of zygotic gene activity.
日本繁殖生物学会の論文
1995-05-01