Classification of Bovine Follicles Based on the Concentrations of Steroids, Glucose and Lactate in Follicular Fluid and the Status of Accompanying Follicles
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概要
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A simple and clear means to identify the physiological status of follicles is essential for study of follicular biology. In the present study, we verified a novel classification procedure based on analysis of the follicular population and glucose concentration in follicular fluid (FF) as an alternative method to classify bovine follicles. Paired ovaries were collected from heifers, and the number of follicles and stage of the CL were recorded. Follicles were initially divided into the following 3 groups according to diameter and the ratio of E2 and P4 (E/P): E2 active (E-A: E/P≥1), E2 inactive (E-I: E/P<1, ≥8.5 mm) and small follicles (E/P<1, <8.5 mm). E-A follicles were easily identified as E2-rich dominant follicles and were further classified according to diameter and stage of the CL as early dominant (EDF: <8.5 mm), dominant (DF: ≥8.5 mm, stages I-III) or preovulatory follicles (POF: ≥8.5 mm, stage IV). E-I follicles were classified as follows based on the status of the accompanying follicles: early atretic (EAF: without an E-A follicle), mid-atretic (MAF: with an EDF or DF) and late atretic follicles (LAF: with an EAF or POF). The follicular P4 concentrations of the MAF and LAF were significantly higher compared with that of the EAF, while follicular glucose concentration of the LAF was lower compared with those of EAF and MAF, indicating that this classification can be used to distinguish early atretic follicles from more advanced atretic follicles. Small follicles were classified as growing (GF: without E-A follicles) and suppressed small follicles (SSF: with E-A follicles). The SSF was easily identifiable by this procedure, but some GF populations likely contained SSF. To identify true GF, the ratio of E2 in the GF and accompanying EAF may be used. In conclusion, analysis of the follicular population in conjunction with biochemical indices such as steroid and glucose concentrations in FF provides a simple and accurate means of classifying bovine follicles.
- 2009-04-01
著者
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HAMANO Seizo
Animal Bio-Technology Center, Livestock Improvement Association of Japan, Inc.
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TETSUKA Masafumi
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medici
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Nishimoto Hiromi
Department of Agricultural and Life Science, Obihiro University of Agriculture & Veterinary Medicine
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Miyamoto Akio
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine
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Tetsuka Masafumi
Department Of Life Science And Agriculture Obihiro University Of Agriculture And Veterinary Medicine
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Tetsuka Masafumi
Department Of Agriculture And Life Science Obihiro University Of Agriculture And Veterinary Medicine
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Tetsuka Masafumi
Dep. Of Agricultural And Life Sci. Obihiro Univ. Of Agriculture And Veterinary Medicine
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Tetsuka Masafumi
Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin
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Nishimoto Hiromi
Department Of Life Science And Agriculture Obihiro University Of Agriculture And Veterinary Medicine
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HILL Glen
Department of Human Sciences, Obihiro University of Agriculture and Veterinary Medicine
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Hamano Seizo
Animal Biotechnology Center Livestock Improvement Association Of Japan
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Hamano Seizo
家畜改良事業団・家畜バイオテクノロジーセンター
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Hill Glen
Department Of Human Sciences Obihiro University Of Agriculture And Veterinary Medicine
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Miyamoto Akio
Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Amaya Montoya
帯広畜産大学 畜産学研究科畜産衛生学
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宮本 明夫
帯広畜産大学・畜産科学科
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Miyamoto A
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Hamano Seizo
Animal Bio-technology Center Livestock Improvement Association Of Japan Inc.
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Tetsuka Masafumi
Department Of Agricultural And Life Science Obihiro University Of Agriculture & Veterinary Medic
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Nishimoto Hiromi
Department Of Agricultural And Life Science Obihiro University Of Agriculture & Veterinary Medic
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Miyamoto Akio
Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Veterinary Medicine
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Miyamoto Akio
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Hamano Seizo
Animal Bio-technology Center Livestock Improvement Association
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