Subjecting Holstein Heifers to Stress During the Follicular Phase Following Superovulatory Treatment May Increase the Female Sex Ratio of Embryos
スポンサーリンク
概要
- 論文の詳細を見る
The sex ratio of mammals has previously been shown to be affected by maternal stress. In our previous study, the proportion of female embryos collected from superovulated and artificially inseminated Holstein heifers that were frequently placed in stanchions and subjected to transrectal examinations of the ovaries during the follicular phase tended to be higher than the expected 50%. The goal of the present study was to test the validity of this observation using a greater number of heifers. Superovulated heifers were artificially inseminated at 56 and 72 h after PGF2α treatment using a single batch of frozen semen. Frequent capture (FC), transrectal examination and/or blood sampling were performed at 4-h intervals from 36 to 76 h after PGF2α treatment (n=13). Nine heifers were used as the Control (non-treatment). Seven-day-embryos were recovered by uterine flushing. Male and female embryos were separated using the loop-mediated isothermal amplification procedure. The proportion of female transferable embryos in the FC group (67.8%, 78/115) was significantly higher than that in the Control group (51.2%, 43/84, P<0.05). The peak concentration of plasma cortisol during the follicular phase following superovulatory treatment was 20.6 ng/ml in the FC group. These results suggest that subjecting heifers to stress during the follicular phase following superovulatory treatment may increase the female sex ratio of embryos.
- 日本繁殖生物学会の論文
- 2009-10-01
著者
-
青柳 敬人
全農etセンター
-
青柳 敬人
北海道家畜改良事業団北見事業所試験研究室
-
青柳 敬人
Ja全農 飼料畜産中央研究所
-
出田 篤司
全農ETセンター
-
Miyamoto Akio
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine
-
Aoyagi Yoshito
Zen-noh Embryo Transfer Center
-
青柳 敬人
全農飼料畜産中央研究所
-
Miyamoto Akio
Graduate School Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
-
Ideta Atsushi
Zen-noh Embryo Transfer Center
-
Urakawa Manami
Zen-noh Embryo Transfer Center
-
KAWASHIMA Chiho
Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Med
-
Kawashima Chiho
Field Centre Of Animal Science And Agriculture Obihiro University Of Agriculture And Veterinary Medi
-
HAYAMA Koh
Zen-noh Embryo Transfer Center
-
Kawashima Chiho
Field Center Of Animal Science And Agriculture Obihiro University Of Agriculture And Veterinary Medi
-
Miyamoto Akio
Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Veterinary Medicine
-
Aoyagi Yoshito
Zen-noh Et Center Hokkaido 080-1407 Jpn
-
Aoyagi Yoshito
Zen-noh Et Center
-
Kawashima Chiho
Field Center Of Animal Sci. Obihiro Univ. Of Agriculture And Veterinary Medicine Hokkaido 080-8555 J
関連論文
- Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia: The Role of Follicular Stimulating Hormone
- Classification of Bovine Follicles Based on the Concentrations of Steroids, Glucose and Lactate in Follicular Fluid and the Status of Accompanying Follicles
- Vascular Changes in the Corpus Luteum During Early Pregnancy in the Cow
- 植氷までの時間と脱グリセリン温度がウシ凍結胚の生存および受胎に及ぼす影響
- ウシの過剰排卵処置におけるPMSGとFSHの比較,とくに血漿ステロイドホルモンの動態と胚回収成績について
- ウサギ卵管へのウシ配偶子移植後の卵分割について
- 牛の胚ならびに卵胞内卵子の非凍結低温下での短期保存について
- ウシ体細胞を用いた遺伝子ターゲティングとノックアウトウシ作製
- 異種抗原を完全に除去したウシの作製(第105回日本外科学会定期学術集会)
- ウシ体細胞を用いた遺伝子ターゲティングの試み
- ウシ胚性幹細胞様細胞を核体とした核移植に関する検討
- ウシ胎仔線維芽細胞を核体とした除核成熟卵子との電気融合率に関する検討
- ヒトリンパ球のウシリンパ球に対する免疫学的反応性に関する研究
- ウシα1-3 galactosyltransferaseのCOS-7細胞への導入
- ウシ胎児線維芽細胞におけるαGal抗原に関する検討
- O-305 和牛α1-3Galactosyltransferase (α1-3GT) c-DNAの異種細胞(COS7)に対する導入 : α1-3GTノックアウトウシの作製
- ウシ初期胚からクローンを作る (特集 クローン動物--その意義と展望)
- 第1または第2減数分裂中期ウシ卵子を用いた顕微授精後の発育能の比較検討
- 核移植によるクローン牛の作出について
- 経膣採卵技術の現場への普及にむけて
- 超音波診断装置を用いた経膣採卵による個体別ウシ受精胚の作出, 移植および受胎率
- グリセリンと蔗糖を用いたマウス胚の急速凍結法に関する研究
- ウシ卵胞内卵子の体外成熟と体外受精におよぼす卵胞培養時間の影響
- 牛の過剰排卵誘起処置に対する卵巣反応に影響を及ぼす要因
- 核移植由来黒毛和種牛におけるマイクロサテライトDNA多型領域の解析
- ウシ初期胚の核移植--ドナ-細胞の由来の違いがウシ核移植由来胚の発育率ならびに受胎率におよぼす影響について (1997年度日本繁殖生物学会公開シンポジウム講演論文 初期胚,胚細胞そして体細胞から個体を作る試み--クロ-ン動物作出研究の意義)
- Prostaglandin F_ Differentially Affects mRNA Expression Relating to Angiogenesis, Vasoactivation and Prostaglandins in the Early and Mid Corpus Luteum in the Cow
- eCG/hCG処理した未成熟雌マウスの卵巣におけるPin1(ペプチジルプロピルイソメラーゼ)の発現
- Basal Levels and GnRH-induced Responses of Peripheral Testosterone and Estrogen in Holstein Bulls with Poor Semen Quality
- 雌仔ウシおよび成熟雌ウシの末梢血中プロジェステロンとエンドセリン濃度の変化 : PGF_投与の影響
- Angiotensin II Secretion by the Bovine Oviduct is Stimulated by Luteinizing Hormone and Ovarian Steroids
- Distribution of Arteriolovenous Vessels, Capillaries and eNOS Expression in the Bovine Corpus Luteum During the Estrous Cycle : a Possible Implication of Different Sensitivity by Luteal Phase to PGF_ in the Increase of Luteal Blood Flow
- A novel concept for the bovine luteolytic cascade with a focus on luteal blood flow and vasoactive factors
- Effect of Suckling on the Reproductive Performance and Metabolic Status of Obese Japanese Black Cattle during the Early Postpartum Period
- Local mechanisms for luteolysis in the cow : Novel roles of vasoactive substances in the luteolytic cascade within the corpus luteum
- Metabolic Profiles in Ovulatory and Anovulatory Primiparous Dairy Cows during the First Follicular Wave Postpartum
- Genital Blood Flow and Endometrial Gene Expression During the Preovulatory Period after Prostaglandin F_-Induced Luteolysis in Different Luteal Phases in Cows
- Distribution of Arteriolovenous Vessels, Capillaries and eNOS Expression in the Bovine Corpus Luteum During the Estrous Cycle: a Possible Implication of Different Sensitivity by Luteal Phase to PGF2 α in the Increase of Luteal Blood Flow
- Changes in the Messenger RNA Expressions of the Endothelin-1 and Angiotensin Systems in Mature Follicles of the Superovulated Bovine Ovary
- Colour Doppler Sonography of Cystic Ovarian Follicles in Cows
- Effect of Intraluteal Injection of Endothelin Type A Receptor Antagonist on PGF_-induced Luteolysis in the Cow
- Comparative Activities of Growth Hormone and Luteinizing Hormone in the Direct Stimulation of Local Release of Progesterone from Microdialyzed Ovine Corpora Lutea In Vivo
- Effective Oxytocin Treatment on Placental Expulsion after Foaling in Heavy Draft Mares(Theriogenology)
- Relationship between Peripartal Plasma Oxytocin and Prostaglandin F_ Metabolite and Placental Expulsion Time in Heavy Draft Mares
- A Potential Use of Color Ultrasound as a Tool for Reproductive Management : New Observations Using Color Ultrasound Scanning that were not Possible with Imaging Only in Black and White
- Relationship between the First Ovulation within Three Weeks Postpartum and Subsequent Ovarian Cycles and Fertility in High Producing Dairy Cows
- Subjecting Holstein Heifers to Stress During the Follicular Phase Following Superovulatory Treatment May Increase the Female Sex Ratio of Embryos
- Relationships among Estrous Behavior, Superovulatory Response and Grade 1 Embryo Sex Ratio in Superovulated Holstein Heifers
- Prostaglandin F2α Differentially Affects mRNA Expression Relating to Angiogenesis, Vasoactivation and Prostaglandins in the Early and Mid Corpus Luteum in the Cow
- Expression of mRNA for Cell Adhesion Molecules in the Bovine Corpus Luteum During the Estrous Cycle and PGF_-Induced Luteolysis
- Induction of Ovulation with GnRH and PGF_ at Two Different Stages During the Early Postpartum Period in Dairy Cows : Ovarian Response and Changes in Hormone Concentrations
- 生後性成熟過程のウシ潜在精巣におけるα-SMAおよび vimentin の免疫組織化学的局在パターンの異常
- ウシ初期胚の核移植 : レシピエント細胞の除核操作後の核の有無が核移植由来胚の発育に及ぼす影響について
- ウシにおいて排卵前の卵胞内でコルチゾールの局所産生が増加する生体内での証拠
- ウシ卵管上皮細胞が分泌する可溶型N-アセチルグルコサミニルトランスフェラーゼ1:LH,VEGFおよびTNFαによる分泌刺激効果
- A Simple Superovulation Method of A Single Injection of Follicle- Stimulating Hormone Combined with Equine Chorionic Gonadotropin for Superovulation of Suffolk Ewes During the Breeding Season: I. Effects of Different Treatments on the Endocrine Profiles
- 1999年度島村賞受賞講演論文 Paracrine/autocrine機構による反芻家畜の卵胞・黄体機能調節に関する研究
- Intraluteal Release of Progesterone and Prostaglandins during PGF2α-Induced Luteolysis in Ewes: Local Effects of Tumor Necrosis Factor-α
- Active Immunization against Inhibin Improves Superovulatory Response to Exogenous FSH in Cattle
- Differential Effect of Follicle-Stimulating Hormone and Estradiol on Expressions of Vascular Endothelial Growth Factor (VEGF) 120, VEGF164 and Their Receptors in Bovine Granulosa Cells
- Testicular Blood Flow and Plasma Concentrations of Testosterone and Total Estrogen in the Stallion after the Administration of Human Chorionic Gonadotropin
- Comparison of Estrus Induction and Subsequent Fertility with Two Different Intravaginal Devices in Ewes during the Non-Breeding Season
- 供試するウシ胚の違いが胚性幹細胞様コロニー形成に及ぼす影響について
- Changes in Plasma Metabolic Hormone Concentrations During the Ovarian Cycles of Japanese Black and Holstein Cattle
- In Vivo Evidence that Local Cortisol Production Increases in the Preovulatory Follicle of the Cow
- Metabolic Profiles in Ovulatory and Anovulatory Primiparous Dairy Cows During the First Follicular Wave Postpartum
- Early Morphological Nuclear Events and Developmental Capacity of Embryos Reconstructed with Fetal Fibroblasts at the M or G1 Phase after Intracytoplasmic Nuclear Injection in Cattle
- Production of Trophoblastic Vesicles Derived From Day 7 and 8 Blastocysts of In Vitro Origin and the Effect of Intrauterine Transfer on the Interestrous Intervals in Japanese Black Heifers
- ウシ成熟卵胞を微透析した灌流液中におけるGnRH-immunoreactivityの存在
- ウシ体外受精卵の胚盤胞への発生に及ぼす卵丘細胞との共培養における合成培地へのグルコース添加の影響
- ウシ体外成熟卵子の人為的活性化処理による胚盤胞への発育に関する研究 : 直流パルス,Caイオノホアおよびサイクロヘキシマイドを用いた複合活性処理について
- ウシ体外受精由来胚の胚盤胞への発育に関する合成培地の検討
- Changes in Follicular Vascularity during the First Follicular Wave in Lactating Cows
- ウシ卵胞発育中の顆粒層細胞におけるアンジオポエチン-Tie系のmRNA発現
- Comparative Activities of Growth Hormone and Luteinizing Hormone in the Direct Stimulation of Local Release of Progesterone from Microdialyzed Ovine Corpora Lutea In Vivo
- Local Release of Progesterone and Oxytocin from Microdialyzed Corpus Luteum in Superovulated Ewes : Characterization during the Non-breeding Season
- A Simple Superovulation Method of A Single Injection of Follicle-Stimulating Hormone Combined with Equine Chorionic Gonadotropin for Superovulation of Suffolk Ewes During the Breeding Season : II. The Ovarian Responses and the Embryo Qualities
- A Simple Superovulation Method of A Single Injection of Follicle-Stimulating Hormone Combined with Equine Chorionic Gonadotropin for Superovulation of Suffolk Ewes During the Breeding Season : I. Effects of Different Treatments on the Endocrine Profiles
- ウシ胚由来培養細胞を核体とした核移植に関する検討
- Exposure of C57BL/6J Male Mice to an Electric Field Improves Copulation Rates with Superovulated Females
- ホルスタイン種乳牛に対するProgesterone releasing intravaginal deviceの応用
- 日本短角種牛の繁殖における過剰排卵 : 胚移植(MOET)の有効性
- 乳牛の過剰排卵処置成績と卵巣内小卵胞数との関係
- と畜場で採取した牛卵巣の肉眼的所見--Midcycle Follicleの検討
- 乳牛の過剰排卵処置における抗PMSG血清の応用
- Relationships Between the First Ovulation Postpartum and Polymorphism in Genes Relating to Function of Immunity, Metabolism and Reproduction in High-producing Dairy Cows
- Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone
- Intraluteal Release of Progesterone and Prostaglandins during PGF_-Induced Luteolysis in Ewes : Local Effects of Tumor Necrosis Factor-α
- N-acetylglucosaminyltransferase I Activity of Bovine Oviduct Epithelial Cells : Stimulation by Luteinizing Hormone, Vascular Endothelial Growth Factor and Tumor Necrosis Factor α
- CaイオノホアA23187によるウシ体外受精由来胚からの内部細胞塊の分離
- Detection of Zearalenone and Its Metabolites in Naturally Contaminated Porcine Follicular Fluid by Using Liquid Chromatography-Tandem Mass Spectrometry
- Changes in Plasma Metabolic Hormone Concentrations during the Ovarian Cycles of Japanese Black and Holstein Cattle
- 生体微透析システムを用いてウシ黄体内に灌流したPGF2α、TPAおよびカルシウムイオノフォアA23187が黄体のProgesterone放出に及ぼす直接的影響
- Basal Levels and GnRH-induced Responses of Peripheral Testosterone and Estrogen in Holstein Bulls with Poor Semen Quality
- ウシ初期胚の核移植 : ドナー細胞の由来の違いがウシ核移植由来胚の発育率ならびに受胎率におよぼす影響について
- Regulation of Trophoblast-Specific Factors by GATA2 and GATA3 in Bovine Trophoblast CT-1 Cells
- Expression and Potential Role of GATA6 in Ruminant Trophoblasts during Peri-implantation Periods
- Functions of interferon tau as an immunological regulator for establishment of pregnancy
- 反芻動物の着床期胚・栄養膜細胞における転写因子GATA6の発現と機能
- Early Morphological Nuclear Events and Developmental Capacity of Embryos Reconstructed with Fetal Fibroblasts at the M or G1 Phase after Intracytoplasmic Nuclear Injection in Cattle