Regulation of Trophoblast-Specific Factors by GATA2 and GATA3 in Bovine Trophoblast CT-1 Cells
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概要
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Numerous transcription factors that regulate trophoblast developmental processes have been identified; however, the regulation of trophoblast-specific gene expression has not been definitively characterized. While a new role of Gata3 in trophoblast development was being demonstrated in mice, we examined effects of GATA transcription factors on conceptus interferon tau (IFNT), a major trophectoderm factor in ruminants. In this study, expression patterns of trophoblast ASCL2, CDX2, CSH1, ELF5, HAND1, IFNT, and TKDP1 mRNAs were initially examined, from which ASCL2, CDX2, IFNT, and TKDP1 mRNAs were found to be similar to those of GATA2 and GATA3 in days 17, 20, and 22 (day 0=day of estrus) bovine conceptuses. A chromatin immunoprecipitation (ChIP) assay revealed that endogenous GATA2 and GATA3 occupied GATA binding sites on the upstream regions of CSH1, IFNT, and TKDP1 genes and on the intron 1 region of CDX2 gene in bovine trophoblast CT-1 cells. In transient transfection analyses of the upstream region of bovine CSH1, and IFNT or the intron 1 region of CDX2 gene, over-expression of GATA2 induced transactivation of these trophoblast-specific genes in bovine non-trophoblast ear fibroblast EF cells, but over-expression of GATA3 did not substantially affect their transactivation. In CT-1 cells, endogenous CDX2 and IFNT mRNAs were down-regulated by GATA2 siRNA, while endogenous ASCL2 and CDX2 mRNAs were down-regulated by GATA3 siRNA. Our results indicate that in addition to trophectoderm lineage specification, GATA2 and/or GATA3 are involved in the regulation of trophoblast-specific gene transcription in bovine trophoblast CT-1 cells.
- 2011-08-01
著者
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青柳 敬人
全農etセンター
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青柳 敬人
北海道家畜改良事業団北見事業所試験研究室
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青柳 敬人
Ja全農 飼料畜産中央研究所
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出田 篤司
全農ETセンター
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Aoyagi Yoshito
Zen-noh Embryo Transfer Center
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青柳 敬人
全農飼料畜産中央研究所
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Ideta Atsushi
Zen-noh Embryo Transfer Center
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Imakawa Kazuhiko
Laboratory Of Animal Breeding And Reproduction Department Of Veterinary Medical Sciences Graduate Sc
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Imakawa K
Univ. Tokyo Tokyo Jpn
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Imakawa Kazuhiko
Laboratory Of Animal Breeding Graduate School Of Agricultural And Life Sciences The University Of To
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BAI Hanako
Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of
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SAKURAI Toshihiro
Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of
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SOMEYA Yohhei
Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of
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KONNO Toshihiro
Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of
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Someya Yohhei
Laboratory Of Animal Breeding Graduate School Of Agricultural And Life Sciences The University Of To
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Bai Hanako
Laboratory Of Animal Breeding Graduate School Of Agricultural And Life Sciences The University Of To
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Aoyagi Yoshito
Zen-noh Et Center Hokkaido 080-1407 Jpn
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Aoyagi Yoshito
Zen-noh Et Center
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Sakurai Toshihiro
Laboratory Of Animal Breeding Graduate School Of Agricultural And Life Sciences The University Of To
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Konno Toshihiro
Laboratory Of Animal Breeding Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Sakurai Toshihiro
Laboratory Of Animal Breeding And Reproduction Veterinary Medical Sciences Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Bai Hanako
Laboratory Of Animal Breeding And Reproduction Veterinary Medical Sciences Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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唄 花子
東京大学大学院農学生命科学研究科獣医学専攻
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