ウシ胎仔線維芽細胞を核体とした除核成熟卵子との電気融合率に関する検討

概要

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We studied electrofusion of bovine karyoplast-cytoplast complexes in order to define the parameters which would result in a high yield of fused embryos in nuclear transfer, using bovine fibroblast cells. Experiment 1. The effects of the number of electrical pulses (1～20 times) to the cytoplasmic membrane of matured enucleated oocytes in vitro were examined. The number of damaged oocytes increased with higher pulse frequency of 10～20 times (100 V/mm, 60 μsec). Experiment 2. The effects of the number of electrical pulses (1,3 or 5 times) to the fusion rates of cytoplasts (recipient enucleated oocytes) and karyoplasts (donor cells) derived from fetal fibroblast cells were examined. The difference in fusion rate was not significant among the three electrical pulse frequencies. These results demonstrate that the increase of the pulse frequency cause more damage to cytoplasmic membrane while not affecting the fusion rate.
東京女子医科大学の論文
2001-12-25