二次性上皮小体機能亢進症患者より得られた上皮小体細胞の同種免疫原性に関する検討

概要

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Parathyroid hypofunction is the complication which is occasionally encountered after the surgical treatments of secondary hyperparathyroidism. Allogeneic parathyroid cell transplantation is thought to be a promising way to treat the hypofunction syndrome of parathyroid gland. In the present study, the evaluation of the allogeneicity of parathyroid gland cells was investigated. To evaluate the allogeneicity of human parathyroid cells, flow cytometry and lymphocyte proliferation test (LPT) were done. Allogeneic human peripheral blood mononuclear cells (PBMC) were cultured with PBMC, or parathyroid cells (PTC) isolated from the patients with secondary hyperparathyroidism. After 5 days of in vitro culture, allogeneic human PBMC were analyzed by flow cytometry for the phenotypic changes in CD3/CD25 double positive cells, or ^3H-thymi.dine uptake tests were done for LPT. T cells of allogeneic PBMC expressed higher level of CD25 when stimulated by PBMC rather than by PTC. ^3H-thymidine uptake was greater when stimulated by PBMC rather than by PTC. In conclusion, the allogeneicity of human parathyroid cells was less both in CD25 expression and LPT than that of PBMC.
東京女子医科大学の論文
2000-03-25