Local Release of Progesterone and Oxytocin from Microdialyzed Corpus Luteum in Superovulated Ewes : Characterization during the Non-breeding Season
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概要
- 論文の詳細を見る
To characterize the local release of progesterone (P) and oxytocin (OT) within the corpus luteum (CL) formed after superovulation during the non-breeding season in ewes, a microdialysis system (MDS) was implanted in corpora lutea (CcL). Superovulation was induced with a combination of PMSG, FSH and GnRH injections in ewes (n=5) that had been pretreated with P using an intravaginal sponge. On Day 5 after GnRH injection, the microdialysis capillary membrane (cut off Mr=1000 kDa) was surgically implanted into the individual CL of both ovaries, and continuously perfused with a Ringers solution up to Day 17. Serial fractions were collected every 30 min on Days 6-7, Days 9-10, Days 12-13 and Day 15, to observe in detail the release patterns of P and OT at different ages of the CL. Both P and OT were released into the MDS in a pulsatile manner throughout the experiment. The frequency of P pulses changed by the age of the CL (P<0.05); it decreased temporally on Days 12-13, but gradually increased on Day 15. The percentages of concomitant P release (coincident rate of pulses) decreased on Days 12-13, but extremely increased to 52.3% on Day 15 (P<0.01). On the other hand, the profiles of OT pulses were constant throughout the experiment. The absolute P concentrations gradually decreased as the CL age progressed, while the absolute OT concentrations remained constant. A high concomitant rate of OT pulses with a P pulse within individual lines of the MDS was observed on Days 6-7 when plasma P concentrations were still rapidly increasing, but such a close relationship between OT pulses and a P pulse disappeared thereafter (P<0.05). Furthermore, a 3 h-infusion with LH on Day 10 stimulated P release during the infusion, whereas a PGF2α infusion induced an inhibition of P release after the infusion (P<0.05). These observations characterized the profiles of a local release of P and OT within an individual CL formed after superovulation during the non-breeding season. The characteristics of P release, but not those of OT release, change with the CL age. Collectively, the data appear to be well comparable to those in the cyclic CL during the breeding season. Thus, a local secretory function of the CL formed after superovulation during the non-breeding season is likely to have a close similarity to those of the cyclic CL.
- 日本繁殖生物学会の論文
- 1995-11-01
著者
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FUKUI Yutaka
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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Fukui Yutaka
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Y
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn
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MIYAMOTO AKIO
Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Obihiro University of
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Fujise Yoshihiro
Inst. Cetacean Res. Tokyo Jpn
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Amaya Montoya
帯広畜産大学 畜産学研究科畜産衛生学
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Fukui Yutaka
The Institute Of Cetacean Research
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Miyamoto A
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Miyamoto Akio
Laboratory Of Animal Genefics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Fukui Y
The Institute Of Cetacean Research
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Taguchi Kiyoshi
Veterinary Surgery Obihiro University Of Agriculture And Veterinary Medicine
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TAKASE Shun-ichi
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Med
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TASHIRO Yumiko
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Med
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KONARI Atsuhiko
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Med
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TOMINO Mikiko
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Med
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ABE Noritsugu
Veterinary Surgery, Obihiro University of Agriculture and Veterinary Medicine
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Tomino Mikiko
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Abe Noritsugu
Veterinary Surgery Obihiro University Of Agriculture And Veterinary Medicine
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Tashiro Y
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Konari Atsuhiko
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Takase Shun-ichi
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Miyamoto Akio
Graduate School Of Animal And Food Hygiene Obihiro Univ. Of Agriculture And Veterinary Medicine
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Fukui Yutaka
Laboratory Of Animal Genefics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Miyamoto Akio
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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