顕微授精後のブタ体外培養胚におけるWell of the Well(WOW)システムの影響
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概要
- 論文の詳細を見る
For developmental competence of porcine embryos in vitro, it is important to improve the culture environment. The present study was performed to evaluate four different culture systems for in vitro matured porcine oocytes following intracytoplasmic sperm injection (ICSI); drop, well and two sizes of the well of the well (WOW) systems (500 and 1,000 μm in diameter). The cleavage rate on Day 2 and the mean cell number in blastocysts on Day 6 were not significantly different among the four treatments. However, the 1,000 μm WOW (24.6%) resulted in a significantly higher (P<0.05) blastocyst rate than those in the other culture systems (12.9, 14.8, and 7.1% for drop, well, and 500 μm WOW, respectively). The present study indicates that the microenvironment created by the 1,000 μm diameter WOW improves blastocyst production of in vitro matured porcine oocytes after ICSI, and that the effectiveness of the WOW system is dependent on the size (diameter) of the WOW.
- 日本繁殖生物学会の論文
著者
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FUKUI Yutaka
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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岩山 広
帯広畜産大学 家畜増殖学研究室
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福井 豊
帯広畜産大学家畜育種増殖学講座
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福井 豊
Laboratory of Meat Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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IWAYAMA Hiroshi
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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高 美貴子
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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岩山 広
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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TAKA Mikiko
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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