Fertility after Different Artificial Insemination Methods Using a Synthetic Semen Extender in Sheep
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概要
- 論文の詳細を見る
The present study aimed to investigate the fertility of ewes artificially inseminated with three different methods using a synthetic semen extender, AndroMed. The three methods of artificial insemination (AI) were cervical AI with fresh-diluted or frozen-diluted semen at observed estrus, and an intrauterine AI with frozen-thawed semen. A total of 80 ewes were treated with a controlled internal drug release (CIDR) containing 0.3 g progesterone per device for 12 days. In Experiment 1 (26 Suffolk ewes), superovulation was induced with 20 mg follicle-stimulating hormone and 250 IU equine chorionic gonadotropin (eCG) two days and one day before CIDR removal, respectively, during the non-breeding season. In Experiment 2 (54 Suffolk and Suffolk crossbred ewes), an intramuscular injection of 500 IU eCG was administered one day before CIDR removal to synchronize estrus and ovulation during the breeding season. In Experiment 1, fresh-diluted or frozen-thawed semen was deposited into the cervical orifice after estrus detection, and an intrauterine AI with frozen-thawed semen was performed by laparoscopy at a fixed-time basis without estrus detection. Embryos were recovered by uterine flushing 6 days after AI, and the rates of recovered, fertilized (cleaved) ova and embryos at the morula or blastocyst stage were compared among the three AI methods. In Experiment 2, the pregnancy rates after the three AI methods were compared. In Experiment 1, the rates of recovered ova were not significantly different among the three AI methods (52.5-56.7%). The rate of fertilized ova (81.0%) by laparoscopic AI with frozen-thawed semen was significantly higher compared with cervical AI of fresh-diluted (25.5%) or frozen-thawed (3.5%) semen, but the rate of embryos at the morula or blastocyst stage (17.6%) was significantly lower than that of the cervical AI with fresh-diluted semen (69.2%). The rates of ewes yielding fertilized ova were not significantly different among the three groups (44.4, 11.1 and 62.5% for cervical AI with fresh-diluted and frozen-thawed semen and intrauterine AI with frozen-thawed semen). In Experiment 2, the pregnancy rate of ewes intrauterinally inseminated with frozen-thawed semen (72.2%) was significantly higher than those of ewes inseminated cervically with fresh-diluted (5.5%) or frozen-thawed (0.0%) semen. The present results showed that acceptable fertilization and pregnancy rates could be obtained by an intrauterine AI with frozen-thawed semen using a synthetic semen extender (AndroMed), but not sufficient by the cervical AI with either fresh or frozen semen.
- 日本繁殖生物学会の論文
- 2009-02-01
著者
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FUKUI Yutaka
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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KOHNO Hirohide
National Livestock Breeding Center, Tokachi Station
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OKABE Kentaro
National Livestock Breeding Center, Tokachi Station
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TOGARI Tetsuro
Hokkaido Prefectural Station of Agriculture
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Fukui Yutaka
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Okabe Kentaro
National Livestock Breeding Center Tokachi Station
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Kohno Hirohide
National Livestock Breeding Center Tokachi Station
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Fukui Y
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fujise Yoshihiro
Inst. Cetacean Res. Tokyo Jpn
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HIWASA Mami
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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Hiwasa Mami
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Yutaka
Department Of Food Production Science Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Yutaka
Laboratory Of Animal Genefics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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