Effects of Bovine Serum Albumin and Trehalose in Semen Diluents for Improvement of Frozen-Thawed Ram Spermatozoa
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概要
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In this study, two following experiments were performed to improve post-thaw motility and viability of frozen-thawed ram spermatozoa. We examined i) the effects of different concentrations of bovine serum albumin (0, 0.3, 1, 5, 10 and 15% BSA) in semen diluents lacking egg yolk and ii) the effects of four semen diluents, fructose (F: control) and trehalose (T) in semen diluents containing egg yolk, 15% BSA in semen diluents without egg yolk (BSA), and modified phosphate buffered saline (m-PBS). Frozen-thawed spermatozoa were examined for progressive sperm motility, viability, morphological abnormality, sperm tail swelling test, and sperm acrosome integrity. In Experiment 1, the rates of sperm motility immediately after thawing (0 h) were significantly (P<0.05) higher in the 10% and 15% BSA groups (55.0 ± 2.9% and 58.3 ± 6.7%, respectively) than in the positive control (F) group (41.7 ± 4.4%). The rate of sperm viability in the negative control (0% BSA) group (80.2 ± 3.3%) was significantly (P<0.05) lower than in the positive control (F) group (89.8 ± 1.5%), but when compared with the F group, no significant differences were found among the 0.3, 1, 5, 10, and 15% BSA groups at 0 h. The rates of sperm morphological abnormality of the 10 and 15% BSA groups (6.5 ± 1.3% and 6.3 ± 1.1%, respectively) were significantly (P<0.05) lower at 0 h than that in the 1% BSA group (16.3 ± 5.2%). In Experiment 2, T addition improved (P<0.05) the post-thaw motility compared with the F and BSA groups. Furthermore, at 3 and 6 h, the post-thaw motility of the T group (36.3 ± 2.4% and 25.0 ± 2.0%, respectively) was significantly (P<0.05) higher than in the BSA (26.3 ± 2.4% and 18.8 ± 1.3%, respectively) and F (28.8 ± 3.8% and 18.8 ± 2.4%, respectively) groups. The post-thaw sperm motility and viability in the m-PBS group were significantly (P<0.05) lower than those of the control (F), T, and BSA groups throughout all observation points. These results indicate that 10% and 15% BSA can be substituted for egg-yolk for ram semen diluent and that the addition of trehalose enhances motility and viability of ram spermatozoa after freezing and thawing.
- 日本繁殖生物学会の論文
- 2006-10-01
著者
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FUKUI Yutaka
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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KOHNO Hirohide
National Livestock Breeding Center, Tokachi Station
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Fukui Yutaka
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Kohno Hirohide
National Livestock Breeding Center Tokachi Station
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MATSUOKA Taiki
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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Fukui Y
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fujise Yoshihiro
Inst. Cetacean Res. Tokyo Jpn
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Matsuoka Taiki
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Imai Hiroko
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Yutaka
Department Of Food Production Science Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Yutaka
Laboratory Of Animal Genefics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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