Effects of the Presence and the Numbers of Corpora Lutea in Non-Delivered and Delivered Pigs on In Vitro Oocyte Maturation and Embryonic Development
スポンサーリンク
概要
- 論文の詳細を見る
The aim of this study was to investigate the effects of the presence and the numbers of corpora lutea (CL) in porcine ovaries on in vitro oocyte maturation and embryonic development following intracytoplasmic sperm injection (ICSI). At oocyte collection, the ovaries of non-delivered and delivered pigs were classified into four groups by CL presence. The effect of the number of CL was also investigated following re-division of the non-delivered groups into four groups. In addition, the progesterone (P4) concentrations in follicular fluid (FF) of all the groups were measured to confirm the relationship between the presence and numbers of CL. Throughout the present study, the oocytes recovered from the CL-holding ovaries showed high (P<0.05) oocyte maturation rates, blastocyst rates and P4 concentrations in FF. Furthermore, in the non-delivered groups, the blastocyst rates and P4 concentrations in FF seemed to coincide with the CL numbers in each ovary. From these findings, we concluded that the presence and number of CL in the ovary can be used as an indicator for estimation of the developmental competence of porcine oocytes. Additionally, the present study suggests that P4 in FF influences in vitro oocyte maturation and embryonic development in porcine in vitro production.
- 日本繁殖生物学会の論文
- 2009-12-01
著者
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WATANABE Hiroyuki
Department of Internal Medicine, Division of Cardiovascular Medicine, Akita University Faculty of Me
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WATANABE Hiroyuki
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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FUKUI Yutaka
Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine
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Yoshizawa Masahiro
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Yoshizawa Masahiro
Department Of Biotechnology Tokyo University Of Agriculture & Technology
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Fukui Y
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fujise Yoshihiro
Inst. Cetacean Res. Tokyo Jpn
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Watanabe H
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Yutaka
Department Of Food Production Science Obihiro University Of Agriculture And Veterinary Medicine
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Watanabe Hiroyuki
Department Of Cardiology Akita University School Of Medicine
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Watanabe Hiroko
Laboratory Of Animal Genetics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Fukui Yutaka
Department Of Animal Science Obihiro University Of Agriculture And Veterinary Medicine
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Watanabe Hiroyuki
Department Of Agricultural Chemistry Ibaraki University
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