Attempt at Intracytoplasmic Sperm Injection of In Vitro Matured Oocytes in Common Minke Whales (Balaenoptera acutorostrata) Captured During the Kushiro Coast Survey
スポンサーリンク
概要
- 論文の詳細を見る
The present study was conducted during the Kushiro Coast Survey in an attempt to produce common minke whale embryos. In Experiment1, we attempted to determine the appropriate culture duration (30 or 40 h) for in vitro maturation (IVM) of immature oocytes using the Well of the Well method. In Experiment 2, and intracytoplasmic sperm injection (ICSI) was applied to matured oocytes from prepubertal and adult common minke whales after IVM culture (40 or 48 h), and then their embryonic development was assessed. In Experiment 1, the maturation rate of oocytes cultured for 40 h (30.4%) was significantly higher than that of oocytes cultured for 30 h (6.8%; P<0.01). In Experiment 2, a total of 35 and 46 immature oocytes derived from adult (n=2) and prepubertal (n=6) minke whales, respectively, were cultured for 40 or 48 h. The maturation rate in the oocytes from the adult whales (34.2%) tended to be higher than that of the oocytes from the prepubertal whales (19.6%), but there was no significant difference. Following ICSI, 3 out of the 10 inseminated and cultured oocytes from the adult whales cleaved (2-, 8-, and 16-cell stages); all of these oocytes had been matured for 40 in culture. However, these oocytes did not develop to further stages. Only one of the 6 oocytes derived from the prebuertal whales, IVM cultured for 40 h and inseminated, developed to the 4-cell stage. The present results indicate that a 40 h IVM culture produces significantly higher rates of in vitro maturation than a 30 h IVM culture for common minke whale oocytes. Following ICSI, some oocytes cleaved to the 16-cell stage, but no further development was observed.
- 日本繁殖生物学会の論文
- 2007-08-01
著者
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加藤 秀弘
東京海洋大学大学院
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Fujise Yoshihiro
The Institute of Cetacean Research
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HOCHI Shinichi
Faculty of Textile Science and Technology, Shinshu University
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HIRABAYASHI Masumi
National Institute for Physiological Sciences
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Hirabayashi Masumi
自然科学研究機構生理学研究所
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Hirabayashi Masumi
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Hirabayashi Masumi
信州大学 繊維
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大隅 清治
日本鯨類研究所
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FUKUI Yutaka
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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HOCHI Shinichi
Graduate School of Science and Technology, Shinshu University
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ISHIKAWA Hajime
The Institute of Cetacean Research
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OHSUMI Seiji
The Institute of Cetacean Research
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Ohsumi S
The Institute Of Cetacean Research
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Ohsumi Seiji
Institute Of Cetacean Research
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Ohsumi Seiji
Seas Fisheries Research Laboratory
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Fukui Yutaka
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Y
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn
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加藤 秀弘
東京海洋大学海洋科学部
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Kato Hidehiro
Tokyo Univ. Marine Sci. And Technol. Tokyo Jpn
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Kato Hidehiro
Tokyo University Of Marine Science And Technology
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Kato Hidehiro
Whales Research Institute
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Hochi Shinichi
Fac. Of Textile Sci. And Technol. Shinshu Univ.
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Hochi Shinichi
Faculty Of Textile Science And Technology Shinshu University
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NAGAI Hiroki
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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MOGOE Toshihiro
The Institute of Cetacean Research
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IWAYAMA Hiroshi
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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MATSUOKA Taiki
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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KOMA Noriko
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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KATO Hidehiro
Laboratory of Marine Biology, Tokyo University of Marine Science and Technology
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Hirao Masao
Ina Research Laboratory Kitayama Labes Co. Ltd.
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Fukui Y
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Nagai Hiroki
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Iwayama Hiroshi
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fujise Yoshihiro
Inst. Cetacean Res. Tokyo Jpn
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Ishikawa H
The Institute Of Cetacean Research
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Koma Noriko
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Kato Hidehiro
Laboratory Of Marine Biology Tokyo University Of Marine Science And Technology
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Matsuoka Taiki
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Iwayama Hiroshi
The Institute Of Cetacean Research
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Fukui Yutaka
The Institute Of Cetacean Research
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Fukui Yutaka
Department Of Food Production Science Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Y
The Institute Of Cetacean Research
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Ohsumi S
Inst. Cetacean Res. Tokyo Jpn
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大隅 清治
東海区水産研究所
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Fukui Yutaka
Laboratory Of Animal Genefics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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加藤 秀弘
鯨類研究所
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