4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
スポンサーリンク
概要
- 論文の詳細を見る
The ability of frozen-thawed boar spermatozoa obtained from epididymides stored at 4 C for 1 day to produce piglets after Fallopian insemination or in vitro fertilization (IVF) of in vitro matured (IVM) oocytes was examined. To test their in vivo fertilization ability, frozen-thawed spermatozoa from 2 boars, which had already shown IVF ability, were introduced into the Fallopian tubes of estrus-synchronized gilts. Embryo collection on the 7th day post-insemination revealed that one of two batches of spermatozoa had an ability of fertilization in vivo and that fertilized oocytes could develop to blastocysts. Three of 6 gilts inseminated with one batch of spermatozoa became pregnant and one of them farrowed 2 normal piglets (1 male and 1 female). IVM oocytes fertilized in vitro by the same spermatozoa were transferred to 3 recipients (200 oocytes per recipient). One of them became pregnant and farrowed 5 normal piglets (3 males and 2 females). The results indicate that boar spermatozoa collected from epididymides stored for 1 day at 4 C and then frozen have the ability to produce piglets. The new cryopreservation protocol and reproductive technology described here can enhance conservation of boar genetic resources when the collection site of the epididymides is far from a laboratory.
- 日本繁殖生物学会の論文
- 1999-10-01
著者
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HIRABAYASHI Masumi
YS New Technology Institute, Inc.
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Ueda Masatsugu
YS New Technology Institute Inc., Shimotsuga-gun, Tochigi 329-0512, Japan
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Kikuchi Kazuhiro
Department Of Internal Medicine School Of Medicine Tokai University
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Kikuchi Kazuhiro
東京農工大学 大学院農学府
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Ueda M
The Ys Institute Inc.
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Ueda Masatsugu
The Ys Inst. Inc.
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Ueda Masatsugu
ワイエスニューテクノロジー研究所
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Nagai Takashi
Department of Internal Medicine, Public Tomioka General Hospital
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HIRABAYASHI Masumi
National Institute for Physiological Sciences
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Hirabayashi M
National Inst. For Physiological Sciences
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Hirabayashi Masumi
自然科学研究機構生理学研究所
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Hirabayashi Masumi
信州大学 繊維
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Kikuchi Kazuhiro
Dep. Of Animal Sci. National Inst. Of Agrobiological Sciences
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永井 卓
(独)・農業・生物系特定産業技術研究機構・畜産草地研究所
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菊地 和弘
Department of Genetic Resources II, National Institute of Agrobiological Resources, Tsukuba, Ibaraki
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柏崎 直巳
School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 229-8501, Japan
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永井 卓
Department of Animal Production, Tohoku National Agricultural Experiment Station, Morioka, Iwate 020
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Noguchi Junko
Department of Genetic Resources II, National Institute of Agrobiological Resources, Tsukuba, Ibaraki
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Shimada Arata
Department of Genetic Resources II, National Institute of Agrobiological Resources, Tsukuba, Ibaraki
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Takahashi Riichi
YS New Technology Institute, Inc., Ishibashi, Shimotsuga, Tochigi 329-0512, Japan
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Shino Masao
School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 229-8501, Japan
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Kaneko Hiroyuki
Department of Genetic Resources II, National Institute of Agrobiological Resources, Tsukuba, Ibaraki
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Hirao Masao
Ina Research Laboratory Kitayama Labes Co. Ltd.
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Takahashi R
The Ys Institute Inc.
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Ueda Masatsugu
Ys New Technology Institute
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Kikuchi Kazuhiro
Department Of Animal Science National Institute Of Agrobiological Sciences
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Nagai Takashi
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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KASHIWAZAKI Naomi
School of Veterinary Medicine, Azabu University
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KANEKO Hiroyuki
Division of Animal Sciences, National Institute of Agrobiological Sciences
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Kaneko Hiroyuki
Division Of Animal Sciences National Institute Of Agrobiological Sciences
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SHINO Masao
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
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TAKAHASHI Ri-ichi
The YS Institute Inc.
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Kaneko H
Division Of Animal Sciences National Institute Of Agrobiological Sciences
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Kashiwazaki Naomi
Laboratory Of Animal Reproduction Azabu University
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Kashiwazaki N
School Of Veterinary Medicine Azabu University
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Kashiwazaki Naomi
School Of Veterinary Medicine Azabu University
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Kashiwazaki Naomi
Laboratory Of Animal Reproduction Graduate School Of Veterinary Science Azabu University
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Noguchi J
Division Of Animal Sciences National Institute Of Agrobiological Sciences
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Noguchi Junko
Positron Medical Center Tokyo Metropolitan Institute Of Gerontology
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Noguchi Junko
Department Of Applied Chemistry Faculty Of Engineering Nagoya University
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Shino Masao
Laboratory Of Animal Reproduction School Of Veterinary Medicine Azabu University
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Shino Masao
School Of Veterinary Medicine Azabu University
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Nagai T
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Hirabayashi Masumi
Ys New Technology Institute Inc.
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Nagai Takuya
Nasu Institute Nisshin Flour Milling Co. Ltd.
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Kaneko Hiroyuki
Department Of Animal Production Kyushu National Agricultural Experiment Station
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Shimada Arata
Department Of Genetic Resources Ii National Institute Of Agrobiological Resources
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Ueda Masatsugu
The Ys Institute Inc.
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Ueda Masatsugu
Ys New Tchnology Inst. Inc.
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