体外受精由来ウシ胚盤胞のガラス化保存においてバイオプシー後の培養時間が融解後の生存性に与える影響
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概要
- 論文の詳細を見る
The aim of this study was to improve the cryosurvival of bovine blastocysts following biopsy. In vitro-derived bovine blastocysts were biopsied with a metal blade, then cultured for 0 to 20 h in m-SOF and then vitrified in the presence of ethylene glycol and dimethyl sulfoxide. The post-warm survival rates after 24 h of in vitro culturing of the biopsied embryos were significantly higher at 1.25, 2.5, 5 and 10 h than that of 0 and 20 h (69, 73, 78 and 64% vs 34 and 27%, p<0.05, respectively). The survival rate of embryos vitrified at 2.5 and 5 h after biopsy was not different from that of the non-vitrified control group (73 and 78% vs 96%, p=0.08 vs 0.07, respectively). These results indicate that 1.25 to 10 h culture between biopsy and vitrification improves the viability of bovine blastocysts produced in vitro.
- 日本繁殖生物学会の論文
著者
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平林 真澄
生理学研究所 行動・代謝分子解析センター
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伊藤 和美
信州大学 繊維
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伊藤 和美
ワイエスニューテクノロジー研究所
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伊藤 和美
YS New Technology Institute Inc.
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HIRABAYASHI Masumi
YS New Technology Institute, Inc.
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堰本 晃代
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321-4415, Japan
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平林 真澄
YS New Technology Institute Inc., Shimotsuga-gun, Tochigi 329-0512, Japan
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Hochi Shinichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda,
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Kimura Ken
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda,
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Ueda Masatsugu
YS New Technology Institute Inc., Shimotsuga-gun, Tochigi 329-0512, Japan
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Nagao Yoshikazu
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321-4415, Japan
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ITO Kazumi
YS New Technology Institute, Inc.
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Sekimoto Akiyo
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321-4415, Japan
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