Expression of Porcine FSHβ Subunit Promoter-driven Herpes Simplex Virus Thymidine Kinase Gene in Transgenic Rats
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概要
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A transgenic rat was established using the construct of porcine FSHβ subunit promoter, the -852/+10 bp region, fused to a Herpes simplex virus thymidine kinase (HSV-TK) gene. Integration of the transgene was confirmed by PCR of tail DNA. RT-PCR of total RNAs of the pituitary, gonad, cerebellum, liver, kidney, adrenal gland, prostate, and uterus revealed that FSHβ was only expressed in the pituitary. Analysis of the expression of reporter gene, HSV-TK, using two specific primer sets revealed that different transcripts were present in the pituitary and testis. The transcript initiated at the transcription initiation site of the porcine FSHβ gene was detected in the pituitary, and another within the TK gene was found in the testis, indicating ectopic testis-specific expression. Immunohistochemistry of the pituitary glands of the transgenic rats for FSH and HSV-TK demonstrated that the FSH-producing cells also produced HSV-TK. The results indicated that the -852/+10 bp region of the FSHβ promoter contains an element(s) that determines the tissue-specific expression. We succeeded in producing FSHβ promoter-driven HSV-TK transgenic rats and were the first time to do so using an animal other than the mouse. The transgenic rats show male infertility that involves abnormal spermatogenesis. We also observed a decrease in the weight of the testis and epididymis, and both motile and living spermatozoa were absent in the epididymis. Consequently, the FSHβ-HSV-TK transgenic rat will provide a useful model for studies on FSH function and male infertility.
- 日本繁殖生物学会の論文
著者
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Kato T
Institute Of Reproduction And Endocrinology Meiji University
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KATO Yukio
Division of Pharmaceutical Sciences, Graduate School of Natural Science & Technology
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TSUKAMURA Hiroko
Laboratory of Animal Reproduction, Nagoya University School of Agricultural Sciences
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MAEDA Kei-ichiro
Laboratory of Animal Reproduction, Nagoya University School of Agricultural Sciences
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ITO Kazumi
YS New Technology Institute, Inc.
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SUSA Takao
Division of Life Science, Graduate School of Agriculture, Meiji University
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KATO Takako
Institute of Reproduction and Endocrinology, Meiji University
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NAKAYAMA Michie
Division of Life Science, Graduate School of Agriculture, Meiji University
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CAI Li-yi
Division of Life Science, Graduate School of Agriculture, Meiji University
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IZUMI Shun-ichiro
Department of Obstetrics and Gynecology, Tokai University, School of Medicine
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AIKAWA Satoko
Division of Life Science, Graduate School of Agriculture, Meiji University
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OHTA Akihiko
Division of Life Science, Graduate School of Agriculture, Meiji University
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Susa Takao
明治大学 農学部生命科学科
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Aikawa Satoko
Division Of Life Science Graduate School Of Agriculture Meiji University
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Maeda Kei-ichiro
Laboratory Of Animal Reproduction Nagoya University School Of Agricultural Sciences
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Tsukamura Hiroko
Laboratory Of Animal Reproduction Graduate School Of Bioagricultural Sciences Nagoya University
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Tsukamura Hiroko
Laboratory Of Animal Reproduction Graduate School Of Agricultural Sciences Nagoya University
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Susa Takao
Division Of Life Science Graduate School Of Agriculture Meiji University
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Kato Yukio
Division Of Life Science Graduate School Of Agriculture Meiji University
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Ito Kazumi
Division Of Pharmaceutical Sciences Graduate School Of Natural Science And Technology Kanazawa Unive
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Kato T
Waseda Univ. Tokyo Jpn
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Cai Li-yi
Division Of Life Science Graduate School Of Agriculture Meiji University
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Maeda Kei-ichiro
Laboratory Of Animal Reproduction Graduate School Of Agricultural Sciences Nagoya University
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Sato T
Inst. Of Reproduction And Endocrinology Meiji Univ.
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Kato Takako
Institute Of Reproduction And Endocrinology Meiji University
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Kato Takako
Institute Of Plasma Physics Nagoya University
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Izumi Shun-ichiro
Department Of Obstetrics And Gynecology School Of Medicine Keio University
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Ohta Akihiko
Division Of Gastroenterology Department Of Internal Medicine Toho University Ohashi Hospital
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Ito Kazumi
Ys New Technology Institute
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Nakayama Michie
Division Of Life Science Graduate School Of Agriculture Meiji University
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Maeda Kei-ichiro
Laboratoey Of Animal Reproduction Graduate School Of Bioagricultural Sciences Nagoya University
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Izumi Shun-ichiro
Department Of Obstetrics And Gynecology
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Ito Kazumi
YS New Technology Inst., Inc.
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MAEDA Kei-Ichiro
Laboratory for Neural Architecture, Graduate School of Bioagricultural Sciences, Nagoya University
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TSUKAMURA Hiroko
Laboratory for Neural Architecture, Graduate School of Bioagricultural Sciences, Nagoya University
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KATO Takako
Institute of Reproduction and Endocrinology, Department of Life Science, Faculty of Agriculture, Meiji University
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